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Karyological and molecular studies were done in this paper on three species of genus Arum; Arum italicum Mill. and Arum maculatum L., with two varieties, and Arum alpinum Schott and Kotschy, also with two varieties. The main goal of this paper was to establish whether they were regularly determined exclusively on the principle of morphological parameters. Karyological studies showed that the number of chromosomes for Arum italicum Mill. amounted to 2n=84, for Arum maculatum L. 2n=56 and for Arum alpinum Schott and Kotschy 2n=28. This confirmed that these species are not only clean and separated, but also support the regularity of the morphological determination. Molecular studies, e.g. RAPD method showed that two genetically separated species groups correspond to the three mentioned species. Arum italicum Mill. is the least homogenous species closely related and the variability between populations is high. Arum maculatum L. is more homogenous within the species, two varieties could be differentiated whereas they are closely genetically related and the variability between the populations is too high. Arum alpinum is strongly homogenous and within these species two varieties could also be differentiated. That means that they are closely related and the variability between the populations is very high. These observations mostly coincided with previous morphological investigations.
Leaves of Italian arums were conditioned and kept in vase solution. The conditioning in water solutions of 8-hydroxyquinoline sulphate (8HQS) at concentration of 200 mg‧dm⁻³ and gibberellic acid (GA₃) at concentration of 100 mg‧dm⁻³ lasted for 4 h. Next the leaves were placed in water or in water solutions of benzyladenine (BA) at concentrations of 50 and 100 mg‧dm⁻³. The control consisted of leaves placed in water. The longevity of Italian arums was approx. two weeks. A 4-hour conditioning in gibberellic acid at concentration of 100 mg‧dm⁻³ as well as kept in benzyladenine at concentration of 50 mg‧dm⁻³ extended leaf longevity by 1-2 weeks. The effectiveness of benzyladenine at concentration of 100 mg‧dm⁻³ was only notable after the leaves had been conditioned in a solution of gibberellic acid. Leaf conditioning in an 8HQS solution did not have any effect on their post-harvest longevity except for those which after being conditioned in this preparation were kept in a BA solution at concentration of 50 mg‧dm⁻³. Conditioning of leaves in a solution of gibberellic acid and their kept, without conditioning, in a 50 mg‧dm⁻³ benzyladenine solution inhibited chlorophyll decomposition in them. A drastic loss of weight was recorded in leaves conditioned in 8HQS and kept in water or a BA solution at concentration of 100 mg‧dm⁻³. A considerable weight loss was found to occur in leaves conditioned in GA₃ and kept in water, although it also needs to be emphasized that they retained longevity for over 4 weeks. The smallest weight losses were recorded in leaves conditioned in 8-hydroxyquinoline sulphate and kept in benzyladenine at concentration of 50 mg‧dm⁻³.
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