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We studied phenotypic relationships among 13 samples of two rock mice species:Apodemus mystacinus (Danford and Alston, 1877) from Anatolia (n = 38) andA. epimelas (Nehring, 1902) from the Balkans (n = 71). Cartesian coordinates of landmarks were collected on the skull and on the occlusal projection of the upper molars (18 landmarks). Centroid size (a measure of overall size) suggested that molars vary independently of overall skull size in both species. Discriminant function analysis on relative warp scores classified >80% of specimens into the correct species, with the best results obtained for the ventral aspect of the skull and for molars. Projection of the 1st discriminant function scores against centroid size provided good separation between the two species. Analysis of vector displacements associated with extremes of variation suggested considerable phenetic differences on the ventral side of the skull and in the molar shape of the two species. The great majority of shifts in landmarks were in a longitudinal direction and the rearrangements of molar cusps were more complex than was the case with the cranium. A bivariate plot of the posterior hard palate length against the incisive foramen length separatedA. mystacinus andA. epimelas well.
During this study, 94 specimens (51 males, 43 females) of the wood mouseApodemus sylvaticus (Linnaeus, 1758), the yellow-necked mouseA. flavicollis (Melchior, 1834) and the western broad-toothed mouseA. epimelas (Nehring, 1902) from 20 localities of Greece were karyologically examined. The first two species were found to be widely distributed and their otherwise very similar karyotype (2n=48, FN=48) could be clearly distinguished, based on C-banding pattern. The third species appeared to have a more limited geographical distribution and its karyotype was distinguished from that of the previous two species, since it contained two pairs of small metacentric autosomes (2n=48, FN=52). The chromosomal study further revealed that nine individuals ofA. flavicollis possessed supernumerary B chromosomes (2n=49–50, FN=49–50). Meiotic chromosome preparations revealed that in contrast to sex chromosomes and autosomes, B chromosomes do not participate in bivalent formation. On the other hand, no supernumerary chromosomes were found in the studiedA. sylvaticus andA. epimelas material.
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