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Similary to other fungal species of the genus Alternaria, Alternaria radicina is a major pathogen that infects both the aboveground and underground parts of carrot plants. Changes in the concentrations of phenolic compounds are observed in infected carrot plants. Carrot seedlings were inoculated with the most pathogenic isolates of Alternaria radicina selected in a laboratory test. A phytopathological analysis was performed to determine the health status of carrot plants. The concentrations of phenolic acids in petioles were determined four weeks after inoculation with A. radicina and at harvest. The results of a greenhouse experiment revealed more severe disease symptoms on carrot plants cv. Koral inoculated with A. radicina isolates, compared with cv. Bolero. The content of the predominant phenolic acid (chlorogenic acid) was found to decrease in the leaf stalks of carrots cv. Koral inoculated with A. radicina. A statistically non-significant increase in chlorogenic acid levels was noted in the leaf stalks of carrots cv. Bolero in the experimental and control groups.
The objective of the work was to study the efficacy of carrot seed treatment with antagonistic microorganisms Clonostachys rosea, C. solani, and grapefruit extract (Biosept 33 SL™). Seeds were artificially inoculated with Alternaria radicina spores prior to treatment. The presence of fungi, seed germination and vigour were determined in laboratory tests, whereas seedling emergence and health were determined in sand and soil assays. Clonostachys spp. and grapefruit extract significantly reduced seed infestation with A. radicina and improved seed germination capacity. At a lower concentration of ioculum, treatment with Clonostachys spp. increased percentage of healthy plants in sand assay. At a higher concentration of inoculum, treatment with Biosept 33 SL™ at 0.5% was more effective. Inoculation with pathogen and, in most cases, the following treatments, did not affect total plant emergence in a soil assay. However, pathogen affected significantly a percentage of healthy plants. Treatment with Biosept 33 SL™ at 0.5% as well as with C. rosea increased their numbers significantly at both concentrations of inoculum.
The purpose of the study was to determine the effect of treating carrot seeds with aqueous extract from hemp on their health and germination. Carrot seeds of cv. Berlikumer 2 and Nantejska were soaked for 30 minutes in solutions of aqueous extract from hemp at concentrations of 3, 5, 10 and 20%. Untreated seeds, seeds soaked in distilled water and treated with Super Homai 70 DS constituted control treatments. The aqueous extract from hemp at none of applied concentrations controlled the incidence of Alternaria alternata and A. dauci compared with untreated seeds and seeds soaked in water. Treating seeds of cv. Berlikumer 2 with solution at concentration of 10% and seeds of cv. Nantejska with solutions at concentrations of 3, 5 and 10% decreased their infestation with A. radicina compared with the control. Super Homai 70 DS was the most effective. Application of the extract at concentrations of 3 and 5% improved seed germination capacity and decreased the number of diseased seedlings in both cultivars compared with untreated seeds and seeds soaked in water. Treating seeds with Super Homai 70 DS improved their germination to the highest degree.
Acta Agrobotanica
|
2005
|
tom 58
|
nr 2
163-169
The experiments were carried out in the years 2002 and 2003 on parsley seeds of 6 cultivars: Alba, Berlińska, Cukrowa, Kinga, Lenka, and Vistula. Mycological analysis of parsley seeds showed that the most common inhabitans were fungi from genus Alternaria (mainly A. alternata and A. radicina) and Fusarium, especially F. avenaceum and F. oxysporum. During the glasshouse investigations fungi Alternaria radicina, A. alternata and Fusarium avenaceum were the main reason for parsley damping-off. The highest number of infected seedlings was observed for Berlińska and Kinga, because in both years of experiments these cultivars had the lowest number of healthy seedlings. The highest number of healthy seedlings had cultivars Alba and Lenka, especially in the second year of experiments. In the field experiments not only fungi from genus Alternaria and Fusarium were the most often isolated from diseased parsley seedlings. Fusarium oxysporum was more often isolated from diseased field seedlings than from glasshouse parsley seedlings. Other fungies isolated often from parsley seedlings cultivated in the field were: Pythium spp., Rhizoctonia solani, Cylindrocarpon destructans and Stemphylium botryosum.
The production of toxic metabolites by four isolates of Alternaria radicina and two isolates of A. alternata in rice grains and carrot discs at 1, 10 and 20ºC was investigated. Incubation lasted 21 and 35 days or 14 and 28 days for rice grains and carrot discs, respectively. Accumulation of toxins in inoculated carrot roots stored for 24 weeks and in inoculated dried carrots stored for 48 weeks was also determined. It was found that A. radicina produced radicinin (RAD) and epi-radicinol (epi-ROH), whereas tenuazonic acid (TeA), altertoxin I (ATX I), alternariol (AOH) and alternariol methyl ether (AME) were produced by A. alternata. Although the isolates tested were capable of producing toxins in rice grains at 1ºC, none of them was detected in carrot discs. Accumulation of epi-ROH was observed in carrot roots stored for 24 weeks, whereas decreased amounts of RAD and epi-ROH were observed in dried carrots stored for 48 weeks. No A. alternata toxins were detected in stored carrot roots, whereas trace amounts of AOH were recorded in dried carrots after 32 and 48 weeks of storage.
Vascular storage parenchyma cells of carrot roots were treated with methanol solutions of radicinin and epiradicinol produced by Alternaria radicina and with alternariol and alternariol methyl ether produced by A. alternata at concentrations of 25 µg/ml and 250 µg/ml, as well as culture filtrates of both fungi. Cell ultrastructure was observed by TEM. No visible changes were noted after treatment with 25 µg/ml toxin solutions. The most extensive plication of cell membranes, and sometimes also cell walls, and the formation of numerous vesicles in the cytoplasm, was observed in cells treated with the higher concentration of toxins. Plasma membrane withdrawal and vésiculation, microvacuole formation, and accumulation of plastoglobuli in chromoplasts also occurred. No changes in the structure of endoplasmic reticulum and dictyosomes were noted. The responses of cell structures to particular toxins were nonspecific. Treatment with culture filtrates from A. radicina resulted in the occurrence of osmiophilic, electron-dense substance in the cytoplasm and plastoglobuli. All alterations induced by filtrates were more extensive than those resulting from toxin solutions, but membrane integrity was not disturbed after any of the treatments.
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