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1

Duplex PCR assay for detection and differentiation of pathogenic and vaccine strains of MDV serotype 1

100%
Krol K., Samorek-Salamonowicz E., Kozdrun W., Wozniakowski G.
Bulletin of the Veterinary Institute in Puławy
|
2007
|
tom 51
|
nr 3
To increase the diagnostic capacity of PCR, duplex PCR for the detection of the Meq gene and 132 bp sequence of Marek's disease virus (MDV), serotype 1 has been developed. The reaction enabled the differentiation of vaccine and field strains of MDV among serotype 1 of the virus. Additionally, it gave preliminary information about eventual vaccination with the Rispens strain.
2

Comparison of standard polymerase chain reaction [PCR] with real-time PCR for meq gene of Marek's disease virus

100%
Krol K., Baigent S., Nair V., Kozdrun W., Samorek-Salamonowicz E.
Bulletin of the Veterinary Institute in Puławy
|
2007
|
tom 51
|
nr 1
The aim of this study was to compare a standard polymerase chain reaction (PCR) with real-time PCR for the detection of meq gene for the Marek's disease virus. Forty-one samples were examined in the experiment. In the standard PCR, the product characteristic for field strains (1 062 bp) was obtained in 19 samples (positive samples), in 22 samples no bands on the gel were observed (negative samples). The real-time PCR detected the viral DNA in 36 samples, so in additionally 13 more samples comparing to the standard reaction. The standard PCR revealed the viral DNA in samples at a total concentration of 10-100 ng/µL. The real-time PCR detected the viral DNA in samples at a total DNA concentration of 0.01-100 ng/µL. The compatibility of reactions was 68.2%. The sensitivity of the standard PCR with reference to the real-time PCR was found to be 86%, and the specificity of both methods 100%. Compared with conventional PCR, real-time PCR is definitely more sensitive, reproducible, quick, and has a wide dynamic range. Additionally, the application of a closed system highly reduces a risk of cross-over contamination.
3

Fold recognition insights into function of herpes ICP4 protein

100%
Wyrwicz L. S., Rychlewski L.
Acta Biochimica Polonica
|
2007
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tom 54
|
nr 3
ICP4 is an important factor regulating the life cycle of HSV1. This conserved protein has several molecular functions, including activation of expression of viral late gene transcripts and inhibition of immediate early genes. Although ICP4 and its Alphaherpesvirinae homologs (eg.: IE62 of VZV) have been subjects of various molecular studies, a complete view of their molecular function is lacking. Here we present the results of fold recognition and molecular modelling of ICP4 functional domains. The performed state-of-the-art bioinformatic fold recognition analysis identified a dual helix-turn-helix motif as a binding module of repressor activities (so called region 2 domain). The mapping of distant homology identified that a segment responsible for activation of late gene promoters (region 4) exhibits folding of uracil DNA glycosylase (UDG), but seems to be a non-functional homolog of UDG. Potential implications of the results are discussed.
4

Sequence analysis of meq oncogene among Polish strains of Marek's disease

80%
Wozniakowski G., Samorek-Salamonowicz E., Kozdrun W.
Polish Journal of Veterinary Sciences
|
2010
|
tom 13
|
nr 2
5

Occurrence of main Marek's disease genes during infection of chickens

80%
Wozniakowski G., Samorek-Salamonowicz E., Kozdrun W.
Bulletin of the Veterinary Institute in Puławy
|
2010
|
tom 54
|
nr 2
123-127
The aim of this study was the determination of the occurence of ICP4, pp38, meq, and LAT genes of Marek's disease virus (MDV) and its rate of replication in internal organs of infected chickens. The genes were determined using PCR, while the virus replication was measured by real-time PCR method. The results have shown the presence of ICP4 and pp38 genes starting from the 3rd d p.i. while meq oncogene was detected from the 7 d p.i. During the whole period of the experiment, no presence of latency associated transcript gene (LAT) was found. The virus replicated most intensively in the spleen and bursa of Fabricius then in the thymus, liver, and lungs. The results imply a role of the main genes ICP4 and pp38 in early cytolytic infection of chickens, and the further occurrence of meq oncogene associated with tumourgenesis. The highest dynamics of MDV replication in the lymphoid organs - bursa of Fabricius, thymus, and spleen indicates its stringent association with lymphoid cells and tissues.
6

The clinical course of the infectious laryngotracheitis (ILT) field case in hens and estimation of immunoprophylaxis efficiency

80%
Wieliczko A., Mazurkiewicz M., Piasecki T., Kuszczynski T.
Polish Journal of Veterinary Sciences
|
2004
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tom 07
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nr 2
7

Five year serological study of bovine herpesvirus type-1 in cattle in Lithuania

80%
Jacevicius E., Salomskas A., Milius J., Petkevicius S., Jaceviciene I., Pridotkas G., Mockeliunas R., Malakauskas A., Morkunas M.
Bulletin of the Veterinary Institute in Puławy
|
2010
|
tom 54
|
nr 3
A serological study of BoHV-1 distribution was conducted in Lithuania from 2005 to 2009. Antibody level was measured using a commercial ELISA. For serological examination, 15,368 random blood samples from cattle of different age, gender, and size of herd, which was unvaccinated against IBR, were collected in 37 districts. It was registered that 11.97% of BoHV-1 were seropositive samples. It was also shown that BOHV-1 is most widespread in cattle herds with population >200 individuals (14.79%). Comparison of different sex groups of cattle revealed that the highest number of infected animals was identified in cows (34.64%) and the lowest in bulls (2.01%). In heifers the number of infected animals was 10.01% and in calves - 4.41%. It was shown that seroprevalence of BoHV-1 infection in Lithuania increased with age of animals. The highest prevalence of BoHV-1 (53.98%) was registered in cattle aged more than 7 years.
8

Specific immunoprophylaxis of some viral diseases in cattle

80%
Rola J., Polak M. P., Zmudzinski J. F.
Polish Journal of Veterinary Sciences
|
2005
|
tom 08
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nr 4
9
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Amplification of DNA from bovine herpesvirus type 1

80%
Rola J.
Bulletin of the Veterinary Institute in Puławy
|
1997
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tom 41
|
nr 1
The aim of the study was to adapt PCR for detection of BHV 1 after in vitro multiplication. Primers were synthesised for a fragment of the gIV glycoprotein gene of the reference Cooper strain of BHV 1. Polish BHV 1 isolates obtained in the 1970s from bull semen were examined. A positive amplification occurred for the strains belonging to subtype BHV 1.2 and subtype BHV1.1. No amplification has occurred with the DNA of EHV 1 and Aujeszky’s disease virus, which confirmed the specificity of the primers used. The specificity of amplification was proved by digestion of the PCR products with Alu I, Ava I, Bgl I, and Hind III restriction enzymes. The electrophoretic pattern of the PCR products digested with these enzymes was in conformity with the restriction map of the amplified fragment.
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