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The genetic diversity of Apulian apricot genotypes (Prunus armeniaca L.) assessed using AFLP markers

100%

Ricciardi L., Giorgio V., De Giovanni C., Lotti C., Gallotta A., Fanizza G.

Cellular and Molecular Biology Letters

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2002

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tom 07

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nr 2A

Apricot is an important crop in Italy and, especially in Southern regions, in the last five years numerous plantings using new cultivars and appropriate cultural management have been established. The cultivars available were created in different environments (USA, France, New Zealand, etc), they then often show low adaptability to Italian conditions. However, in the South of Italy, it is still possible to safeguard and to exploit a considerable amount of the apricot genetic variation available in ecotypes often characterised both by useful bio-agronomic traits and by good environmental adaptation. These genetic materials could be used in breeding programs aimed at broadening the harvest period and obtaining high fruit quality and resistance to the main biotic and abiotic stresses.

2

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Application of new biotechnologies in Brassica napus L. resynthesis

84%

Sosnowska K., Szala L., Liersch A., Mikolajczyk K., Ksiazczyk T., Bocianowski J., Wos H., Cegielska-Taras T.

BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology

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2015

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tom 96

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nr 1

3

Random variability of map distances based on Kosambi's and Haldane's mapping functions

84%

Huehn M.

Journal of Applied Genetics

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2010

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tom 51

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nr 1

27-31

The majority of published genetic maps are based on Kosambi distances or on Haldane distances. For a comparison of both map distance measures, their random variability is of particular interest. For the statistic ‘variance’, this paper presents a relationship between Kosambi distances and Haldane distances. The results suggest that Kosambi distances exhibit a smaller random variability. The theoretical results are applied to an experimental data set for molecular AFLP markers linked to the bolting gene of sugar beet (Beta vulgaris L.).

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Low genetic diversity of declining Viola uliginosa (Violaceae) at its southern range limits in Poland

84%

Paul W., Cieslak E., Ronikier M., Migdalek G., Slomka A., Zabicka J.

Acta Biologica Cracoviensia. Series Botanica

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2016

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tom 58

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nr 2

Viola uliginosa (bog violet) is a declining species throughout its range due to – mostly anthropogenic – drying out of the wet habitats it occupies. Using AFLP markers, we aimed to estimate the genetic diversity in Polish populations, that may give an insight into the situation of plant populations facing rapid loss of natural habitats. Bog violet from several dispersed Polish populations is generally characterized by very low genetic diversity (HT = 0.048), even lower than several other endangered violets; therefore, we suggest that it should preserve at least EN rank in the red lists/red data books. The mean gene diversity within all populations (HS) was much lower than gene diversity (GST) between populations (0.020 versus 0.583, respectively) which supports the prevalence of clonal propagation of the species (mainly by stolons) but may also point to some significance of autogamy in cleisto- and chasmogamous flowers. A high FST value and the Mantel test for all populations revealed significant isolation by distance. Geographically neighboring pairs of populations formed genetic clusters supported by all (in the case of two closest populations) or most statistical analyses applied. Special attention should be paid to the locus classicus of the species in Rząska, consisting of a small number of individuals, forming a genetically distinct group, revealing very low gene diversity (Hj = 0.009) and the longest genetic distance to the remaining populations. Our results can contribute to planning future protection measures for the species at this and other locations. Genetic structure of the studied populations suggests local affinities of populations but does not generally support hypothesized recent continuity of V. uliginosa range along the river valleys of southern Poland; this view may, however, be altered with widening of the scope of studied populations and chosen molecular markers.

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An evaluation of a new approach to the regeneration of Helichrysum italicum [Roth] G. Don, and the molecular characterization of the variation among sets of differently derived regenerants

67%

Perrini R., Alba V., Ruta C., Morone-Fortunato I., Blanco A., Montemurro C.

Cellular and Molecular Biology Letters

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2009

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tom 14

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nr 3

377-394

A protocol for the induction of regeneration from leaves of Helichrysum italicum was established. Calli were found to form on the basal medium only when it was supplemented with thidiazuron (TDZ) alone or in combination with naphthalene acetic acid (NAA), with a percentage ranking of at least 80%. The hormone-free medium showed the highest percentage of shoot regeneration (62%) even though no callus formed. AFLP markers were employed to verify tissue culture-induced variation in the regenerated plantlets obtained by direct shoot regeneration or the indirect shoot regeneration process (callus formation). Seven out of the eleven AFLP primer pairs yielded polymorphic patterns. The average number of fragments per primer pair was 64.1. Singletons were represented by 12 (2.7%) fragments. Student’s T-test was performed both on the average number of shared fragments and on the nucleotide diversity, and no significant statistical difference was observed between the two regeneration treatments.

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Low genetic diversity in the endangered population of Viola uliginosa in its locus classicus at Rzaska near Cracow [southern Poland] as revealed by AFLP markers

67%

Cieslak E., Paul W., Ronikier M.

Acta Societatis Botanicorum Poloniae

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2006

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tom 75

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nr 3

245-251

An extremely endangered population of Viola uliginosa Besser at the classical locality of this taxon has been studied. The AFLP analysis was based on 18 specimens of V. uliginosa (about 10% of preserved individuals); additionally, two individuals of V. riviniana were included in the data set as the out group. A high genetical uniformity of the whole population (similarity indexes close to 1) was detected. It was not correlated significantly with the spatial distribution of the plants. The study serves as a basis for practical conservation measures and at the same time as a starting point for a more extensive research on the genetical variability of the species throughout its range.

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Badanie podobienstwa genetycznego pomiedzy formami rodzicielskimi mieszancow liniowych kukurydzy przy uzyciu markerow molekularnych AFLP i RAPD

67%

Broda Z., Tomkowiak A., Molinski K., Adamczyk J.

Biuletyn Instytutu Hodowli i Aklimatyzacji Roślin

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2007

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nr 244

191-201

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Genetic and morphological differentiation between Melica ciliata L. and M. transsilvanica Schur (Poaceae) in Europe reveals the non-presence of M. ciliata in the Polish flora

67%

Szczepaniak M., Cieslak E.

Acta Societatis Botanicorum Poloniae

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2011

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tom 80

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nr 4

A good knowledge of species delimitation is crucial for the biodiversity protection and the conservation of wild species. We studied the efficiency of AFLP markers and morphological characters to assist species determination for Melica ciliata L. and M. transsilvanica Schur within European range of distribution, including isolated and range-limit populations of "M. ciliata" (i.e. M. cf. ciliata) from the Polish Sudetes, where it is regarded as critically endangered. AFLP markers were found to be more effective then morphological characters (more or less continuous) in distinguishing the both studied species. AMOVA revealed very low genetic diversity within populations and high differentiation among populations of M. ciliata and M. transsilvanica (FST = 0.89 and 0.95, respectively). The species-diagnostic AFLP markers of M. transsilvanica shared with "M. ciliata" from the Sudetes were detected. On the other hand, no species-diagnostic genetic markers of M. ciliata or hybrid-diagnostic markers of M. × thuringiaca were found within "M. ciliata". PCoA and NJ showed an overlapping genetic diversity of "M. ciliata" and M. transsilvanica. Hierarchical AMOVA supported the absence of a significant genotypic distinction between "M. ciliata" and M. transsilvanica. ANOVA showed that the length ratio of lower to upper glumes was the best morphological character to discriminate between M. ciliata and M. transsilvanica. Combined morphological and genetic data show that M. ciliata is not currently present in Poland as its putative Polish populations represent M. transsilvanica. A significant decrease in genetic variability that could influence viability was not observed the in Sudetian populations of M. transsilvanica. However, the population size changes significantly as a result of plant succession. Correction of the northern limit of the continuous distribution of M. ciliata L. in Central Europe is presented.

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Rozwazania nad mapowaniem genetycznym QTL odpowiedzialnym za ceche zoltonasiennosci rzepaku ozimego [Brassica napus L.]

59%

Hernacki B., Bartkowiak-Broda I., Piotrowska A., Cegielska-Taras T.

Biuletyn Instytutu Hodowli i Aklimatyzacji Roślin

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2009

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nr 253

221-229

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Ocena zróżnicowania genetycznego linii kukurydzy przydatnych do hodowli mieszańców heterozyjnych przy użyciu markerów molekularnych AFLP i RAPD

51%

Tomkowiak A., Broda Z., Adamczyk J.

Acta Scientiarum Polonorum. Agricultura

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2009

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tom 08

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nr 1

W ostatnich latach w nowoczesnych programach hodowlanych wykorzystuje się tradycyjne metody hodowli w połączeniu z technikami molekularnymi. Daje to możliwość wprowadzenia bardziej obiektywnych kryteriów selekcji i doboru materiału rodzicielskiego, pozwala również w sposób znaczący skrócić czas niezbędny do wyhodowania nowej odmiany. Doświadczenie przeprowadzono w 2006 roku w Rolniczym Gospodarstwie Doświadczalnym w Dłoni (51° N; 17° E), należącym do Uniwersytetu Przyrodniczego w Poznaniu. Celem badań była próba wykazania zależności efektu heterozji mieszańców pokolenia F1 kukurydzy od dystansu genetycznego na poziomie molekularnym pomiędzy komponentami rodzicielskimi, z uwzględnieniem pochodzenia tych komponentów. Wykazanie powyższych zależności pozwoliłoby na wybór form rodzicielskich biorących udział w tworzeniu nowej odmiany, zmniejszając znacznie zakres – liczbę linii testowanych w warunkach polowych, a tym samym znacznie skróciłoby cykl hodowlany i co ważniejsze pomniejszyło koszty hodowli. Markery molekularne AFLP i RAPD mogą być użyteczne w wyborze komponentów rodzicielskich do krzyżowań dla kukurydzy przy jednoczesnym uwzględnieniu pochodzenia tych komponentów.

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Markery molekularne w badaniach rzepaku (Brassica napus L.). I. Przegląd stosowanych technik

43%

Matuszczak M.

Rośliny Oleiste - Oilseed Crops

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2013

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tom 34

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nr 2

Współczesna biologia molekularna dostarcza wciąż nowych metod pozwalających na opracowywanie markerów genetycznych, w tym przede wszystkim markerów molekularnych (markerów DNA). Znajdują one zastosowanie w różnych badaniach dotyczących rzepaku. Ich wykorzystanie w hodowli znacznie przyspiesza uzyskiwanie nowych odmian. Markery molekularne umożliwiają postęp w badaniach genomu. Istotne jest także znaczenie markerów molekularnych w badaniach pokrewieństwa oraz identyfikacji odmian. W pracy dokonano przeglądu technik poszukiwania markerów, które już znalazły lub mogą znaleźć zastosowanie w badaniach nad rzepakiem. Omówiono zarówno metody, które były stosowane na wczesnym etapie rozwoju tej dziedziny, jak i te, które stosowane są obecnie. Zasygnalizowano także prawdopodobne kierunki rozwoju nowych technologii dla pozyskiwania markerów w przyszłości.

Ograniczanie wyników

The genetic diversity of Apulian apricot genotypes (Prunus armeniaca L.) assessed using AFLP markers

Application of new biotechnologies in Brassica napus L. resynthesis

Random variability of map distances based on Kosambi's and Haldane's mapping functions

Low genetic diversity of declining Viola uliginosa (Violaceae) at its southern range limits in Poland

An evaluation of a new approach to the regeneration of Helichrysum italicum [Roth] G. Don, and the molecular characterization of the variation among sets of differently derived regenerants

Low genetic diversity in the endangered population of Viola uliginosa in its locus classicus at Rzaska near Cracow [southern Poland] as revealed by AFLP markers

Badanie podobienstwa genetycznego pomiedzy formami rodzicielskimi mieszancow liniowych kukurydzy przy uzyciu markerow molekularnych AFLP i RAPD

Genetic and morphological differentiation between Melica ciliata L. and M. transsilvanica Schur (Poaceae) in Europe reveals the non-presence of M. ciliata in the Polish flora

Rozwazania nad mapowaniem genetycznym QTL odpowiedzialnym za ceche zoltonasiennosci rzepaku ozimego [Brassica napus L.]

Ocena zróżnicowania genetycznego linii kukurydzy przydatnych do hodowli mieszańców heterozyjnych przy użyciu markerów molekularnych AFLP i RAPD

Markery molekularne w badaniach rzepaku (Brassica napus L.). I. Przegląd stosowanych technik