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Extended-spectrum beta-lactamase-producing Klebsiella pneumoniae in a neonatal unit: control of an outbreak using a new ADSRRS technique

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Krawczyk B., Samet A., Czarniak E., Szczapa J., Kur J.

Polish Journal of Microbiology

2005

tom 54

nr 2

Extended-spectrum β-lactamase (ESBL)-producing Klebsiella pneumoniae (EPKP) strains are frequently implicated in outbreaks in neonatal units. From April 2002 to January 2003, 149 neonates were colonized/infected with EPKP In the Neonatal Clinic of the Teaching Hospital at the Medical University of Gdansk, Poland. A novel assay based on suppression of PCR, ADSRRS-fingerprinting, was successfully evaluated for typing EPKP isolates. The results showed that the genotypes of all outbreak-related strains were identical, which suggested that the outbreak originated from a single clone. This conclusion was confirmed by using different methods - RAPD and PFGE. The outbreak was stopped by adopting improved hygiene and instituting outbreak control measures.

Ograniczanie wyników

1 Polish Journal of Microbiology

1 Czarniak E.

1 Krawczyk B.

1 Kur J.

1 Samet A.

1 Szczapa J.

1 2005

Wyniki wyszukiwania

Extended-spectrum beta-lactamase-producing Klebsiella pneumoniae in a neonatal unit: control of an outbreak using a new ADSRRS technique