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The yellowish-tangerine tomato (cv. Bursztyn) in the green, light yellow and yellow stages of ripening were treated with 0.1% and 1.0% of methyl jasmonate (JA-Me) in lanolin paste and kept for several days and then they were evaluated for production of ethylene, ACC oxidase activity and C02 evolution. Production of endogenous ethylene in mature green fruits was low and increased during ripening. JA-Me stimulated ethylene production and ACC oxidase activity in all investigated stages of fruit ripening. Slices excised from mature green fruits produced highest amount of carbon dioxide as compared to more advanced stages of ripening. JA-Me in 0.1% and 1.0% concentrations increased significantly C02 evolution in green fruits, while in light yellow and yellow fruits only higher concentration of JA-Me stimulated carbon dioxide production. Wpływ estru metylowego kwasu jasmonowego na produkcję etylenu, aktywność oksydazy ACC i wydzielanie dwutlenku węgla w owocach żółto-pomarańczowej odmiany pomidorów
Apples cv. Jonagold were harvested at the beginning of October and stored at 0°C until treatment between the beginning of December and the end of January. Methyl jasmonate (JA-Me) at the concentration of 1.0, 0.5, 0.1, 0.05, and 0.01 % in lanolin paste were applied to the surface of intact apples. During five days from treatment, samples of cortex with skin (area about 2.0 cm2) were cut off at a depth of about 2 mm and used for determination of ethylene production, ACC oxidase activity and respiration determined as CO, evolution. The production of endogenous ethylene was highest at mid-January (100, 280, and 250 nl/g*h at December, mid-January, and the end of January, respectively). During December and at the beginning of January, JA-Me initially (1-2 days after treatment) stimulated ethylene production and then the production was inhibited. The lower concentration of JA-Me caused initially the greater stimulation and then lower inhibition of ethylene production. However, at the time of maximum production of endogenous ethylene (mid-January) and later, stimulatory effect of JA-Me disappeared. The effect of various concentrations and time of application of JA-Me on ACC oxidase activity had similar trend as endogenous ethylene production. Methyl jasmonate stimulated respiration and this effect was dependent on JA-Me concentration and independent on time of application. The metabolic significance of these findings is discussed.
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