Wyniki wyszukiwania

1

Molecular characterization and intracellular regulation of the human serotonin transporter in Caco-2 cells

100%

Iceta R., Mesonero J. E., Aramayona J. J., Alcalde A. I.

Journal of Physiology and Pharmacology

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2006

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tom 57

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nr 1

The serotonin transporter (SERT) has shown itself to be an effective pharmacological target in the treatment of mood disorders and some kinds of gastrointestinal syndromes. Most of the molecular studies of SERT in humans have been carried out using heterologous models. In this work, we have investigated the human enterocyte-like Caco-2 cell line as a potential "in vitro" model to study the human SERT. The results show that these cells express a SERT mRNA identical to the human brain SERT, and a 70 kDa protein immunodetected using a specific antibody. The SERT activity levels in Caco-2 cells increased in correlation with the onset and maintenance of the morphological and functional differentiation of the cells. Caco-2 SERT was also shown to be a high affinity (Kt=0.216 µM) saturable, Na+-dependent transporter that was inhibited by fluoxetine (IC50=17.6 nM). In addition, SERT activity was inhibited by the intracellular modulators protein kinase C and cAMP, either after short or long-term treatment. In short, the expression and molecular characteristics of the human SERT in Caco-2 cells indicate that this cell line may be an ideal tool to study in vitro the physiology and pharmacology of human SERT.

2

Effect of prolactin on the andrenogonadal activity of the male pigeon [Columba livia]

84%

Mukherjee M., Mondal A., Ghosh S., Pakrashi A., Ghosh A.

Folia Biologica

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1993

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tom 41

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nr 1-2

3

Exogenous melatonin delays gastric emptying rate in rats: role of CCK2 and 5-HT3 receptors

84%

Kasimay O., Cakir B., Devseren E., Yegen B. C.

Journal of Physiology and Pharmacology

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2005

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tom 56

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nr 4

Pineal hormone melatonin is proposed as a potential treatment for severe sleep disturbances, and various gastrointestinal disorders. It was shown that melatonin increases intestinal motility and influences the activity of myoelectric complexes of the gut. The aim of the study was to evaluate the mechanisms of the effect of exogenous melatonin on gastric emptying rate. Male Sprague-Dawley rats were fitted with gastric cannulas under anesthesia. The rate of gastric emptying of saline was determined after instillation into the gastric fistula, from the volume and phenol red concentrations recovered after 5 min. Melatonin injected intraperitoneally (ip; 0.001-100 mg/kg) delayed gastric emptying rate of saline at 3 and 10 mg/kg doses. When administered ip 15 min before melatonin (10 mg/kg) injections, CCK2 (L-365,260, 1 mg/kg) or 5-HT3 receptor (ramosetrone, 50 µg/kg) blockers abolished melatonin-induced delay in gastric emptying rate, while the blockade of sympathetic ganglia (bretylium tosylate, 15 mg/kg) significantly reduced the delay in gastric emptying rate. CCK1 receptor blocker (L-364,718, 1 mg/kg) had no significant effect on the delaying action of melatonin. Our results indicate that pharmacological doses of melatonin delay gastric emptying via mechanisms that involve CCK2 and 5-HT3 receptors. Moreover, it appears that exogenous melatonin inhibits gastric motility in part by activating sympathetic neurons.

4

Responses in the hypothalamic monoaminergic system actvity in ewes to beta-endorphin, CRF and their antagonists

59%

Przekop F., Tomaszewska D.

Acta Neurobiologiae Experimentalis

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1996

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tom 56

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nr 3

Ograniczanie wyników

2 Journal of Physiology and Pharmacology

1 Acta Neurobiologiae Experimentalis

1 Folia Biologica

1 Alcalde A. I.

1 Aramayona J. J.

1 Cakir B.

1 Devseren E.

1 Ghosh A.

1 Ghosh S.

1 Iceta R.

1 Kasimay O.

1 Mesonero J. E.

1 Mondal A.

1 Mukherjee M.

1 Pakrashi A.

1 Przekop F.

1 Tomaszewska D.

1 Yegen B. C.

1 2006

1 2005

1 1996

1 1993

Molecular characterization and intracellular regulation of the human serotonin transporter in Caco-2 cells

Effect of prolactin on the andrenogonadal activity of the male pigeon [Columba livia]

Exogenous melatonin delays gastric emptying rate in rats: role of CCK2 and 5-HT3 receptors

Responses in the hypothalamic monoaminergic system actvity in ewes to beta-endorphin, CRF and their antagonists