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Direct in vivo transfer of plasmid DNA into murine tumors: Effects of endotoxin presence and transgene localization

100%

Budryk M., Cichon T., Szala S.

Acta Biochimica Polonica

2001

tom 48

nr 3

The purpose of this study was to investigate the effect of endotoxin presence in plasmid DNA preparations on the efficiency of transfection achieved in vivo with B16(F10) and Renca tumors and to determine transgene localization. Our data show that endotoxin markedly decreases the efficiency of transfection. Furthermore, the transgene transferred in vivo can be found in both neoplastic and normal (most likely myofibroblast) cells lying in proximity of the administration site.

Ograniczanie wyników

1 Acta Biochimica Polonica

1 Budryk M.

1 Cichon T.

1 Szala S.

1 2001

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Direct in vivo transfer of plasmid DNA into murine tumors: Effects of endotoxin presence and transgene localization