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Allium tuncelianum (Tunceli garlic) is a single cloved important threatened endemic geophyte of high commercial importance that grows scattered on hot temperate mountains under oak trees in the eastern Turkish province of Tunceli. Tunceli garlic is used diversely in salads, foods and in pharmaceutical industry. They also offer a rich choice for their use in rock gardens and landscaping. There is need to develop appropriate agronomic practices for its propagation outside its habitat. In line with this objective, the study optimized planting time (1st September, 15th September and 1st October) of A. tuncelianum on mild alkaline soils under semi-arid climatic conditions of Diyarbakir, Turkey. The results of the study showed positive gain in morphological characteristics such that bulb diameter of the plants changed between 3.43 to 4.60 cm, bulb circumference ranged 14.4 to 16.1 cm and bulb weight had a range of 35.9 and 46.3 g. These results underscore positive effects of early and mid September plantings of Tunceli garlic outside its habitat under semi-arid conditions for economic production with early harvest.
Hyacinthus genus is an important group of ornamental plants that bear white, yellow, pink, red or purple coloured flowers. It has about 2000 species spread around the world that are grown commercially. Although, plant occurs naturally in Turkey yet efforts have not been made to adapt it for open field cultivation. There is need to transfer and establish these plants from wild to fields for commercial use through in vitro and ex vitro approaches, that will help local economy profitably. This study reports in vitro culture of Hyacinthus orientalis L. subsp. orientalis; using fruits containing immature zygotic embryos cultured on MS medium containing varying concentrations of Thidiazuron (TDZ) with and without 0.2 mg l-1 naphthaleneacetic acid (NAA) supplemented with 20 or 40 g l-1 sucrose. The study also reports induction of bulblets on tender leaf sheaths on MS medium containing different concentrations of benzylaminopurine (BAP) + 0.1 mg l-1 NAA supplemented with 30 g l-1 sucrose. The maximum bulblet regeneration (40%) with 31.33 bulblets/explant was noted on MS medium containing 0.15 mg l-1 TDZ supplemented with 40 g l-1 sucrose. Whereas, the best bulblet regeneration on tender leaf sheath explants was noted on 1.5 mg l-1 BAP + 0.1 mg l-1 NAA with 2.97 bulblets per explant of 0.55 cm bulb diameter and 1.20 leaves per bulblet. These bulblets were cultured singly on MS medium containing 20 mg l-1 GA3 (Gibberellic acid) + 50 g l-1 sucrose and attained a diameter of 0.75–1.00 cm in 30 days time. The bulbs regenerated on both explants were successfully rooted and acclimatised in plant growth chamber using peat moss followed by their transfer to open field conditions.
Iris species have great economic importance for their use in ornamental and pharmaceutical industry. Flora of Turkey reports about 43 local taxons of which 16 are endemic. Iris suaveolens Boiss. et Reuter is an endemic species with high seed dormancy and has high tolerance to cold and drought. This study focused on seed dormancy break of I. suaveolens under in vitro conditions. In the first experiment, the seeds were given stratification treatment on MS medium containing different concentrations of BAP with or without NAA. The 2nd experiment reports effects of alternative combinations of cold (at 4°C, 24 h dark) – warm (16 h light photoperiod) conditions on medium containing different concentrations of plant growth regulators on agar solidified MS medium. Both experiments showed about 8.33% seed germination against 64.5% seed viability as confirmed by tetrazolium test. All germinating seeds were abnormal and very weak. In the third experiment, 10 minutes acid scarified seeds cultured at 24°C in 16 h light photo period on MS medium achieved 60.0% germination. Similar treatment under cold + dark condition was inhibitory and failed to show identical results. This underlines the importance of acid scarification, photoperiod and warm treatments of I. suaveolens seeds to break seed dormancy. All germinated seeds showed normal growth and development under field conditions.
Cotton (Gossypium hirsutum L.) is the most significant cash crop and backbone of global textile industry. The importance of cotton can hardly be over emphasized in the economy of cotton-growing countries as cotton and cotton products contribute significantly to the foreign exchange earnings. Cotton breeders have continuously sought to improve cotton’s quality through conventional breeding in the past centuries; however, due to limited availability of germplasm with resistant to particular insects, pests and diseases, further advancements in cotton breeding have been challenging. The progress in transformation systems in cotton paved the way for the genetic improvement by enabling the researchers to transfer specific genes among the species and to incorporate them in cotton genome. With the development of first genetically engineered cotton plant in 1987, several characteristics such as biotic (insects, viruses, bacteria and fungi) resistance, abiotic (drought, chilling, heat, salt), herbicide tolerance, manipulation of oil and fiber traits have been reported to date. Genetic engineering has emerged as a necessary tool in cotton breeding programs, strengthening classical strategies to improve yield and yield contributing factors. The current review highlights the advances and endeavors in cotton genetic engineering achieved by researchers worldwide utilizing modern biotechnological approaches. Future prospects of the transgenic cotton are also discussed.
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