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Differences in metabolic profiles of planktonic and biofilm cells in Staphylococcus aureus-1H Nuclear Magnetic Resonance search for candidate biomarkers

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Junka A. F., Deja S., Smutnicka D., Szymczyk P., Ziolkowski G., Bartoszewicz M., Mlynarz P.

Acta Biochimica Polonica

2013

tom 60

nr 4

Staphylococcus aureus is responsible for many types of infections related to biofilm presence. As the early diagnostics remains the best option for prevention of biofilm infections, the aim of the work presented was to search for differences in metabolite patterns of S. aureus ATCC6538 biofilm vs. free-swimming S. aureus planktonic forms. For this purpose, Nuclear Magnetic Resonance (NMR) spectroscopy was applied. Data obtained were supported by means of Scanning Electron Microscopy, quantitative cultures and X-ray computed microtomography. Metabolic trends accompanying S. aureus biofilm formation were found using Principal Component Analysis (PCA). Levels of isoleucine, alanine and 2,3-butanediol were significantly higher in biofilm than in planktonic forms, whereas level of osmoprotectant glycine-betaine was significantly higher in planktonic forms of S. aureus. Results obtained may find future application in clinical diagnostics of S. aureus biofilm-related infections.

Ograniczanie wyników

1 Acta Biochimica Polonica

1 Bartoszewicz M.

1 Deja S.

1 Junka A. F.

1 Mlynarz P.

1 Smutnicka D.

1 Szymczyk P.

1 Ziolkowski G.

1 2013

Wyniki wyszukiwania

Differences in metabolic profiles of planktonic and biofilm cells in Staphylococcus aureus-1H Nuclear Magnetic Resonance search for candidate biomarkers