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The expression of myogenic regulatory factors and muscle growth factors in the masticatory muscles of dystrophin-deficient (MDX) mice

100%

Spassov A., Gredes T., Gedrange T., Lucke S., Pavlovic D., Kunert-Keil C.

Cellular and Molecular Biology Letters

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2011

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tom 16

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nr 2

The activities of myogenic regulatory factors (MRF) and muscle growth factors increase in muscle that is undergoing regeneration, and may correspond to some specific changes. Little is known about the role of MRFs in masticatory muscles in mdx mice (the model of Duchenne muscular dystrophy) and particularly about their mRNA expression during the process of muscle regeneration. Using Taqman RT-PCR, we examined the mRNA expression of the MRFs myogenin and MyoD1 (myogenic differentiation 1), and of the muscle growth factors myostatin, IGF1 (insulin-like growth factor) and MGF (mechanogrowth factor) in the masseter, temporal and tongue masticatory muscles of mdx mice (n = 6 to 10 per group). The myogenin mRNA expression in the mdx masseter and temporal muscle was found to have increased (P < 0.05), whereas the myostatin mRNA expressions in the mdx masseter (P < 0.005) and tongue (P < 0.05) were found to have diminished compared to those for the controls. The IGF and MGF mRNA amounts in the mdx mice remained unchanged. Inside the mdx animal group, gender-related differences in the mRNA expressions were also found. A higher mRNA expression of myogenin and MyoD1 in the mdx massterer and temporal muscles was found in females in comparison to males, and the level of myostatin was higher in the masseter and tongue muscle (P < 0.001 for all comparisons). Similar gender-related differences were also found within the control groups. This study reveals the intermuscular differences in the mRNA expression pattern of myogenin and myostatin in mdx mice. The existence of these differences implies that dystrophinopathy affects the skeletal muscles differentially. The finding of gender-related differences in the mRNA expression of the examined factors may indicate the importance of hormonal influences on muscle regeneration.

2

Comparison of effects of endogenous and exogenous excitatory amino acids on Retzius nerve cells of the leech

100%

Lopicic S., Nedeljkov V., Cemerikic D., Dudvarski Z., Pavlovic D., Cutura N.

Folia Biologica

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2009

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tom 57

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nr 1-2

83-90

3

Talin, vinculin and nestin expression in orofacial muscles of dystrophin deficient mdx mice

100%

Spassov A., Gredes T., Pavlovic D., Gedrange T., Lehmann C., Lucke S., Kunert-Keil C.

Archivum Immunologiae et Therapiae Experimentalis

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2012

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tom 60

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nr 2

4

Correlation of nitric oxide levels in the cerebellum and spinal cord of experimental autoimmune encephalomyelitis rats with clinical symptoms

100%

Ljubisavljevic S., Stojanovic I., Pavlovic D., Milojkovic M., Vojinovic S., Sokolovic D., Stevanovic I.

Acta Neurobiologiae Experimentalis

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2012

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tom 72

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nr 1

Experimental autoimmune encephalomyelitis (EAE) is a well-established cell-mediated autoimmune inflammatory disease of the CNS, which has been used as a model of the human demyelinating disease. EAE is characterized by infiltration of the CNS by lymphocytes and mononuclear cells, microglial and astrocytic hypertrophy, and demyelination which cumulatively contribute to clinical expression of the disease. EAE was induced in female Sprague-Dawley rats, 3 months old (300 g ± 20 g), by immunization with myelin basic protein (MBP) in combination with Complete Freund's adjuvant (CFA). The animals were divided into 7 groups: control, EAE, CFA, EAE + aminoguanidine (AG), AG, EAE + N-acetyl-L-cysteine (NAC) and NAC. The animals were sacrificed 15 days after EAE induction, and the level of nitric oxide (NO') production was determined by measuring nitrite and nitrate concentrations in 10% homogenate of cerebellum and spinal cord. Obtained results showed that the level of NO' was significantly increased in all examined tissues of the EAE rats compared to the control and CFA groups. Also, AG and NAC treatment decreased the level of NO' in all tissues compared to the EAE group. The level of NO' is increased significantly in the spinal cord compared to the cerebellum. The clinical course of the EAE was significantly decreased in the EAE groups treated with AG and NAC during the development of the disease compared to EAE group and its correlates with the NO' level in cerebellum and spinal cord. The findings of our work suggest that NO' and its derivatives play an important role in multiple sclerosis (MS). It may be the best target for new therapies in human demyelinating disease and recommend the new therapeutic approaches based on a decreased level of NO' during the course of MS.

5

Skeletal muscle relaxants inhibit rat tracheal smooth muscle tone in vitro

84%

Milchert M., Spassov A., Meissner K., Nedeljkov V., Lehmann C., Wendt M., Loster B. W., Mazurkiewicz-Janik M., Gedrange T., Pavlovic D.

Journal of Physiology and Pharmacology. Supplement

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2009

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tom 60

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nr 8

5-11

6

Effects of amitriptyline, fluoxetine, tranylcypromine and venlafaxine on rat vascular smooth muscle in vitro – the role of the endothelium

84%

Ribback S., Pavlovic D., Herbst D., Nedeljkov-Jancic R., Wendt M., Nedeljkov V., Bleich S., Frieling H.

Journal of Physiology and Pharmacology

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2012

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tom 63

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nr 2

7

Photosynthetic efficiency of four woody species growing on fly ash deposits of a Serbian "Nikola Tesla" - a thermoelectric plant

84%

Mitrovic M., Jaric S., Kostic O., Gajic G., Karadzic B., Djurdievic L., Oberan L. L., Pavlovic D., Pavlovic M., Pavlovic P.

Polish Journal of Environmental Studies

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2012

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tom 21

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nr 5

Photosynthetic efficiency and damage symptoms of Tamarix tetrandra Pallas, Populus alba L., Robinia pseudoacacia L. (planted), and Amorpha fruticosa L. (naturally colonized) were studied at two fly ash deposit lagoons of the 'Nikola Tesla - A' power plant (Obrenovac, Serbia), weathered 5 (L1) and 13 years (L2). In early phases of weathering, after 5 years, a reduced vitality of populations growing on the ash was noticed in planted R. pseudoacacia and spontaneously populated A. fruticosa (P<0.001) in comparison to T. tentandra and P. alba, due to higher salinity and elevated concentrations of As, Mo, Cu, and Mn in the ash. Thirteen years after planting, as weathering proceeded due to reduced salinity and toxicity, A. fruticosa species showed photosynthesis recovery and had the highest photosynthetic efficiency (P<0.001), suggesting that it poses adaptive capacity to survive and develop tolerance to stress in such habitats that strongly recommend this species for planting at fly ash lagoons.

8

Glutamine and alanyl-glutamine dipeptide reduce mesenteric plasma extravasation, leukocyte adhesion and tumor necrosis factor-alpha [TNF-alpha] release during experimental endotoxemia

68%

Scheibe R., Schade M., Grundling M., Pavlovic D., Starke K., Wendt M., Retter S., Murphy M., Suchner U., Spassov A., Gedrange T., Lehmann C.

Journal of Physiology and Pharmacology. Supplement

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2009

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tom 60

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nr 8

19-24

Ograniczanie wyników

The expression of myogenic regulatory factors and muscle growth factors in the masticatory muscles of dystrophin-deficient (MDX) mice

Comparison of effects of endogenous and exogenous excitatory amino acids on Retzius nerve cells of the leech

Talin, vinculin and nestin expression in orofacial muscles of dystrophin deficient mdx mice

Correlation of nitric oxide levels in the cerebellum and spinal cord of experimental autoimmune encephalomyelitis rats with clinical symptoms

Skeletal muscle relaxants inhibit rat tracheal smooth muscle tone in vitro

Effects of amitriptyline, fluoxetine, tranylcypromine and venlafaxine on rat vascular smooth muscle in vitro – the role of the endothelium

Photosynthetic efficiency of four woody species growing on fly ash deposits of a Serbian "Nikola Tesla" - a thermoelectric plant

Glutamine and alanyl-glutamine dipeptide reduce mesenteric plasma extravasation, leukocyte adhesion and tumor necrosis factor-alpha [TNF-alpha] release during experimental endotoxemia