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1

Geny przydatnosci technologicznej mleka - wyniki testowania buhajow

100%
Kaminski S., Zabolewicz T.
Przegląd Mleczarski
|
1998
|
nr 04
122-124
2

Projekt kompleksowego wykorzystania badan nad genami bialek mleka dla zwiekszenia zawartosci bialka w mleku krow

100%
Kaminski S., Zabolewicz T.
Prace i Materiały Zootechniczne. Zeszyt Specjalny
|
1997
|
nr 07
83-84
3
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
Content available

Genotyping of bovine beta-lactoglobulin [LGB] by PCR-SSCP technique

100%
Kaminski S., Zabolewicz T.
Journal of Applied Genetics
|
1997
|
tom 38
|
nr 4
A new method facilitating the identification of the two most common alleles (A and B) of the bovine beta-lacoglobulin (LGB) gene is described. The method is based on two steps: PCR amplification of 240 bp fragment of LGB gene followed by the single stranded conformation polymorphism (SSCP) detection. AA, AB and BB genotypes of LGB were identified with this technique. The PCR-SSCP is simple, accurate and relatively inexpensive. Additionally, this method has a potential to detect new variants within the amplified gene fragment.
4
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
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Associations between bovine beta-lactoglobulin polymorphism within coding and regulatory sequences and milk performance traits

100%
Kaminski S., Zabolewicz T.
Journal of Applied Genetics
|
2000
|
tom 41
|
nr 2
5
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
Content available

SSCP polymorphism within 5' region of bovine lactoglobulin [LGB] gene

100%
Kaminski S., Zabolewicz T.
Journal of Applied Genetics
|
1998
|
tom 39
|
nr 1
In the paper the detection of the SSCP polymorphism within the 5’ fragment of bovine beta-lactoglobulin (LGB) gene is described. The 5’ fragment of LGB gene (209 bp) was PCR-amplified and then subjected to electrophoresis allowing the detection of SSCP polymorphism. Among 124 animals (50 cows and 74 bulls) six SSCP patterns were identified and named Rl, R2, R3, R4, R5 and R6, which occured with the frequency of 0.32, 0.51, 0.09, 0.06, 0.01 and 0.01, respectively. The PCR-SSCP method is simple, fast, and relatively inexpensive. The SSCP polymorphism reported in the paper may be useful in looking for the associations between different SSCP patterns and LGB gene expression and milk properties.
6

Differential expression of complement subcomponent C1qA in blood samples of healthy and BLV-infected Polish Holstein-Friesian cows

81%
Brym P., Zabolewicz T., Kaminski S.
Animal Science Papers and Reports
|
2018
|
tom 36
|
nr 1
7

Program praktycznego wykorzystania identyfikacji genu kappa-kazeiny oraz genu beta-laktoglobuliny

81%
Kaminski S., Czarnik U., Zabolewicz T.
Przegląd Hodowlany
|
1996
|
tom 64
|
nr 01
4-5
8

Zwiazek miedzy polimorfizmem beta-laktoglobuliny [BLG] i zroznicowaniem wskaznikow diagnostycznych charakteryzujacych podkliniczne stany mastitis u krow rasy czarno-bialej

81%
Walawski K., Czarnik U., Zabolewicz T.
Roczniki Naukowe Zootechniki
|
1997
|
tom 24
|
nr 4
9-22
9

Preliminary evaluation of BM6438 polymorphism as a putative candidate marker for milk performance traits associated with a new breeding concept in Polish Black-and-White cattle

81%
Czarnik U., Walawski K., Zabolewicz T., Pareek C.
Annals of Animal Science
|
2005
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tom 05
|
nr 2
10

Struktura genetyczna polimorficznych wariantow mLYS-MIC [macrophage expressed lysozyme gene microsatellite polimorphism] w krajowej populacji bydla czarno-bialego

81%
Zabolewicz T., Pareek C. S., Galinski M., Walawski K.
Roczniki Naukowe Zootechniki. Suplement
|
2002
|
tom 15
49-54
11

Identyfikacja QTLs cech uzytkowosci mlecznej bydla metoda skanowania genomu i analizy sprzezen genetycznych zgodnie z procedura "selective DNA pooling"

81%
Walawski K., Pareek C. S., Zabolewicz T., Czarnik U.
Biuletyn Informacyjny. Instytut Zootechniki
|
2002
|
tom 40
|
nr 2
75-90
12
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
Content available

Beta-lactoglobulin and kappa-casein polymorphism in relation to production traits and technological properties of milk in the herd of Polish Black-and-White cows

81%
Walawski K., Sowinski G., Czarnik U., Zabolewicz T.
Genetica Polonica
|
1994
|
tom 35
|
nr 1-2
93-108
13

High lysozyme activity families in Polish black and white cattle

81%
Walawski K., Pareek C. S., Czarnik U., Zabolewicz T.
Acta Theriologica
|
1999
|
tom 44
|
nr 1
Recent achievements in specificity of lysozyme gene organization and expression made it possible to consider lysozyme as a candidate gene for natural resistance in cattle. In our research affort on more than 10 000 animals reared in large herds were examined. Obtained results were the background for determining genetic basis of lysozyme activity differentiation. The aim of present investigation was to trace out high lysozyme activity (LZM+) animals. This was achieved by pre-se!ecting animals on the basis of quantitative analysis of lysozyme activity measurements and verifi­cation by single gene allelic segregation. Generally, a very low frequency of LZM+ carriers were identified in a random sampling population. However two sires and some dams were recognized as LZM+ heterozygote. Independent to sex, age and other conditions, the progeny groups alternatively inherited very high or normal lysozyme activity were stated. The obtained results were applied in the experimental breeding program to gain information on the relationship between lysozyme polymorphism and natural resistance specificity in cattle.
14

Evaluation of putative relationship between PRNP octapeptide repeat polymorphism and variability of milk production traits in cattle

71%
Czarnik U., Zabolewicz T., Pareek C. S., Zieminski R., Walawski K.
Annals of Animal Science
|
2006
|
tom 06
|
nr 1
15
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
Content available

Association of polymorphism within LTF gene promoter with lactoferrin concentration in milk of Holstein cows

71%
Zabolewicz T., Barcewicz M., Brym P., Puckowska P., Kaminski S.
Polish Journal of Veterinary Sciences
|
2014
|
tom 17
|
nr 4
This study analyzed the association between single nucleotide polymorphism (A/C) in position -28 located in the TATA box of LTF gene and the lactoferrin concentration in bovine milk secreted by healthy and infected udders. Out of 241, 69 cows were selected into the experimental group and were divided into 3 groups according to mean value of somatic cell count (SCC): I <180 000 cells/mL, II: 180 000 - 350 000 cells/mL and III > 350 000 cells/mL. In each SCC group, three LTF genotypes: AA, AC and CC were identified by PCR-SSCP method. A total of 604 milk samples were collected monthly and lactoferrin concentration was measured by ELISA. The 1-way ANOVA within SCC groups was performed to estimate association of -28 A/C genotypes with mean lactoferrin concentration per lactation. In the group of healthy cows (<180 000 cells/mL) LTF concentration in milk cows with the AA genotype (107.58 ± 17.92 μg/mL) was significantly higher than in homozygotes CC (52.09 ± 19.01 μg/mL). Unexpectedly, in cows with elevated SCC (>350 000 cells/mL) we observed a significant opposite relationship (207.21 ± 28.50 in CC vs 115.0 ± 28.6 μg/mL in AA). We hypothesized that a promoter with allele C, which cannot be recognized as a TATA sequence is becoming more accessible for other transcription factors, which may induce alternative LTF gene expression. We assume that our results demonstrate a very interesting effect of differential gene expression depending on polymorphism in a key regulatory motif (TATA box) and also on the health status of mammary tissues.
16

Effectiveness of a program aimed at the elimination of BLAD-carrier bulls from Polish Holstein-Friesian cattle

71%
Czarnik U., Grzybowski G., Kaminski S., Prusak B., Zabolewicz T.
Journal of Applied Genetics
|
2007
|
tom 48
|
nr 4
375-377
The molecular basis of BLAD is the D128G mutation of the gene coding for the CD18 subunit of beta-2 integrin. This mutation is lethal, since homozygous (BL/BL) animals die before they reach sexual maturity. In the 1990s, BLAD was the most widespread genetic disease in HF cattle worldwide. The aim of the present study was to determine the frequency of BLAD carriers among 4645 young breeding bulls in Poland in 1995-2006. The frequency of carriers of the mutated allele showed a clear decreasing trend. The highest frequency (7.9%) was recorded while implementing the BLAD control program (1995-1997). Regular monitoring has enabled a great reduction of this threat to the tested population. Today only sporadic cases of BL/TL heterozygotes are reported (ca. 0.8% in 2004-2006).
17
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
Content available

Assessment of genomic inbreeding in Polish Konik horses

71%
Kaminski S., Hering D. M., Jaworski Z., Zabolewicz T., Rusc A.
Polish Journal of Veterinary Sciences
|
2017
|
tom 20
|
nr 3
The aim of this study was to assess the inbreeding coefficient of Polish Konik horses based on runs of homozygosity (ROH). Ninety six horses kept in 6 herds located across Poland were genotyped with the use of EquineSNP60 BeadChip (Illumina). SNP markers with a Minor Allele Frequency lower than 0.01 and SNPs assigned to chromosome X or Y were excluded from the study. A total of 50 708 SNPs were included for statistical analysis (SVS software, Golden Helix). The analysis showed that the population is in genetic equilibrium, with Hₑ and Hₒ estimates both equal to 0.3086. Seven categories of Runs of Homozygozity (ROH) length were defined: >0.5, >1, >2, >4, >8, >16, >25 Mb. The genomic inbreeding coefficient derived from ROH (FROH) calculated for each ROH length ranged from 15.96% based on the shortest ROH (>0,5Mb) to 2.71% for the longest ROH (>25Mb). Among individual horses, the inbreeding coefficient ranged from 5.25% to 22.41% (for ROH >1Mb). Analysis of ROH in Polish Koniks allows for more effective management of their inbreeding in the future.
18

Detection of lethal effects linked with the PRNP locus

71%
Walawski K., Wojciechowski R., Czarnik U., Zabolewicz T., Pareek C. S.
Bulletin of the Veterinary Institute in Puławy
|
2006
|
tom 50
|
nr 1
19

DGAT1 K232A quantitative trait nucleotide polymorphism in Polish Black-and-White cattle

71%
Pareek C. S., Czarnik U., Zabolewicz T., Pareek R. S., Walawski K.
Journal of Applied Genetics
|
2005
|
tom 46
|
nr 1
The diacylglycerol o-acyltransferase 1 gene (OGAT1) was investigated in Polish Black-and-White cattle. The frequency of the K allele was 0.60, 0.68 and 0.48 for AI sires (n = 150), young bulls (n = 139) and cows (n = 213), respectively. The method of selective genotyping for identification of the quantitative trait nucleotide was verified through identification of DGAT1 effect on milk production traits. Daughters of six heterozygous bulls were selectively genotyped based on their milk traits. The genotypic frequencies differed between high and low yield groups representing milk and fat contents. The Kruskal-Wallis test revealed a highly significant effect of DGAT1 K232A in cows with extremely low fat content and a significant effect in cows with extremely high protein content of milk. No significant effect of AI sires’ genotypes on their breeding value was found.
20

Silent point mutation polymorphism of the bovine CD18 encoding gene

71%
Czarnik U., Zabolewicz T., Galinski M., Pareek C. S., Walawski K.
Journal of Applied Genetics
|
2004
|
tom 45
|
nr 1
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