Preferencje help
Polish version English version Język
Widoczny [Schowaj] Abstrakt
Liczba wyników

Znaleziono wyników: 6

Liczba wyników na stronie
Pierwsza strona wyników Pięć stron wyników wstecz Poprzednia strona wyników Strona / 1 Następna strona wyników Pięć stron wyników wprzód Ostatnia strona wyników

Wyniki wyszukiwania

help Sortuj według:

help Ogranicz wyniki do:
Pierwsza strona wyników Pięć stron wyników wstecz Poprzednia strona wyników Strona / 1 Następna strona wyników Pięć stron wyników wprzód Ostatnia strona wyników
1

Establishment and characterization of Prosopis laevigata (Humb. & Bonpl. ex Willd) M.C. Johnst. cell suspension culture: a biotechnology approach for mesquite gum production

100%
Trejo-Espino J. L., Rodriguez-Monroy M., Vernon-Carter E. J., Cruz-Sosa F.
Acta Physiologiae Plantarum
|
2011
|
tom 33
|
nr 5
This study presents a protocol for the establishment of Prosopis laevigata cell suspension culture as a strategy to obtain an in vitro mesquite gum productive cell line. The callus used for this purpose was obtained with hypocotyls from 15-day-old plantlets, placed on Murashige–Skoog medium with two different plant growth regulators (PGRs), 2,4,5-trichlorophenoxy acetic acid (2,4,5-T; 5.0 µM) and kinetin (KIN; 5.0 µM). With this PGRs treatment, after four subcultures (30 days each) an exuded gum-like substance was observed on the callus surface. The growth kinetics of the cell suspension culture showed a specific cell growth rate (µ) of 0.14 d⁻¹ and doubling time (td) of 6.6 days, respectively. The gum-like substance from callus culture and the broth from cell suspension culture were subjected to chemical analysis and compared with the mesquite gum exuded from wild trees. Both, gum-like substance from callus culture and the broth from cell suspension culture showed the presence of Arabinogalactanproteins, and their polysaccharide fraction presented the same monosaccharides as those isolated from mesquite gum. In addition, the emulsifying properties of gum-like substance from callus culture and the broth from cell suspension culture were compared to those of mesquite gum and all three samples exhibited similar emulsifying capacity and emulsification stability.
2

In vitro propagation of two antidiabetic species known as guarumbo: Cecropia obtusifolia and Cecropia peltata

100%
del Pilar Nicasio-Torres M., Erazo-Gomez J. C., Cruz-Sosa F.
Acta Physiologiae Plantarum
|
2009
|
tom 31
|
nr 5
Two Cecropia species (Cecropia obtusifolia and C. peltata), known as guarumbo, are employed in Mexican traditional medicine to treat diabetes mellitus; the leaves of both species contain phenolic bioactive compounds such as chlorogenic acid (CA) and isoorientine (ISO), which have been attributed with hypoglycemic, hypolipidemic, and antioxidant properties. An in vitro propagation protocol was developed from existing apical bud meristem from C. obtusifolia seedlings; the shoot generation was induced on Murashige and Skoog (MS) medium supplemented with varying concentrations of 6-benzylaminopurine and kinetin (Kn) combined with either α-naphtalene acetic acid (NAA) or indole-3-acetic acid (IAA) auxins. Best morphogenetic response was developed with Kn 26.64 µM combined with either NAA or IAA 0.57 µM, respectively; likewise, C. peltata-seedling apical buds were subjected to these best selected treatments. Cecropia obtusifolia and C. peltata shoots were rooted in growth regulator-free half-strength MS medium, and regenerated whole plants were adapted successfully under greenhouse conditions and field. Leaves from both Cecropia-micropropagated plants produced the phenolic compounds CA and ISO, with highest concentrations in leaves from 18-month C. obtusifolia (12.28 ± 7.06 mg g⁻¹ dry leaves of CA and 8.30 ± 2.70 mg g⁻¹ dry leaves of ISO) growth in the field. Our results offer a protocol of apical-bud use for multiplication and curative-property conservation of the two previously mentioned important Mexican medicinal plants.
3

Production of potential anti-inflammatory compounds in cell suspension cultures of Sphaeralcea angustifolia (Cav.) G. Don

76%
Nicasio-Torres M. P., Perez-Hernandez J., Gonzalez-Cortazar M., Mackes-Fischer M., Tortoriello J., Cruz-Sosa F.
Acta Physiologiae Plantarum
|
2016
|
tom 38
|
nr 08
4

Production of chlorogenic acid and isoorientin hypoglycemic compounds in Cecropia obtusifolia calli and cell suspension cultures with nitrate deficiency

76%
Nicasio-Torres M. P., Meckes-Fischer M., Aguilar-Santamaria L., Garduno-Ramirez M. L., Chavez-Avila V. M., Cruz-Sosa F.
Acta Physiologiae Plantarum
|
2012
|
tom 34
|
nr 1
The antidiabetic properties of Cecropia obtusifolia are attributed to chlorogenic acid (CGA) and isoorientin (ISO) phenolic compounds; both compounds possess hypoglycemic, hypolipidemic, and antioxidant properties. As a potential strategy for an adequate supply of authentic plant raw material, the aim of this study was to establish in vitro conditions for the development of cell suspension cultures that produce these bioactive compounds. Callus cultures of leaf explants from acclimatized tree and in vitro plantlets were set up using different auxin levels; treatments with 2,4-dichlorophenoxyacetic acid (2,4-D) and a-naphthalene acetic acid (NAA) to 8.92 µM with 6-benzylaminopurine (BAP) at 2.22 µM stimulate highest callus production. Seedling cotyledon, hypocotyl, leaf, and stem explants developed calli bearing roots with 2,4-D. With NAA, hypocotyl, cotyledon, and leaf explants developed morphogenic calli; 75% of stem explants formed calli, and the remaining calli developed shoots. Determined CGA concentrations in calli were similar to those detected in the leaves of wild trees, and ISO was not produced. Cell suspension cultures were established from leaf explants friable calli with 8.92 µM 2,4-D in combination with 2.22 µM BAP, employing 4 and 5% inocula in fresh weight; CGA levels were maintained and ISO was produced only at the end of logarithmic growth. On diminishing nitrate content in Murashige and Skoog (MS) medium to 8.0 mM, maximum cell biomasses diminished, CGA production is increased and twice with 16.0 and, instead of CGA production is tripled and quadrupled with 16.0 and 8.0 mM nitrates, respectively, and ISO synthesis was induced earlier and for a longer time period, increasing its levels at the end of culture. Two compounds with ultraviolet spectra similar to those of caffeic and ferulic acids were formed. Our results offer a protocol of cell suspension cultures for C. obtusifolia bioactive production and hypoglycemic property conservation.
5

Effect of the culture medium and biotic stimulation on taxane production in Taxus globosa Schltdl in vitro cultures

64%
Tapia N., Zamilpa A., Bonfill M., Ventura E., Cruz-Vega D., Del Villar A., Cruz-Sosa F., Osuna L.
Acta Physiologiae Plantarum
|
2013
|
tom 35
|
nr 12
Taxus globosa is the only species of the Taxus genus that grows in Mexico. In this study, callus cultures from leaves and young shoots of T. globosa were established in Gamborg’s B5 medium supplemented with 2,4-dichlorophenoxiacetic acid (2 mg/L), kinetin (0.5 mg/L) and gibberellic acid (0.25 mg/L). Callus growth and taxane production were evaluated using two culture media: Woody Plant Medium and Gamborg’s B5 supplemented with picloram (2 mg/L), kinetin (0.1 mg/L) and gibberellic acid (0.5 mg/L). The effect of the inoculum size (50, 100 and 150 g FW/L) and culture media (Woody Plant Medium and Gamborg’s B5) with and without the presence of methyl jasmonate (100 μM) on T. globosa cell suspensions was assessed. Taxane analysis revealed that the calli in Gamborg’s B5 produced taxol (50 μg/g DW), baccatin III, 10-deacetyl baccatin III and 10-deacetyl taxol. Woody Plant Medium also induced the production of taxol, although to a lesser extent. The optimum inoculum size was 50 g FW/L. In cell suspension cultures, both media had a significant effect on taxane production when supplemented with methyl jasmonate. In Woody Plant Medium, at day 14, a total concentration of 197.999 μg/L of taxol, 160.622 μg/L of baccatin III, 633.724 μg/L of 10-deacetyl baccatin III and 229.611 μg/L 10-deacetyl taxol were obtained, with total excretion of baccatin III and 10-deacetyl taxol to the culture medium. In Gamborg’s B5, cephalomanine was obtained at a concentration of 91.428 μg/L without elicitation, and all taxanes were excreted to the medium to a variable extent.
6

Taxane production induced by methyl jasmonate in free and immobilized cell cultures of Mexican yew (Taxus globosa Schltdl)

64%
Osuna L., Tapia N., Cusido R., Palazon J., Bonfill M., Zamilpa A., Lopez-Upton J., Cruz-Sosa F.
Acta Physiologiae Plantarum
|
2015
|
tom 37
|
nr 10
Taxus globosa Schltdl, the Mexican yew, represents a new source of taxanes, including taxol, baccatin III, 10-deacetylbaccatin III, 10-deacetyltaxol and cephalomannine. Due to the anticancer activity and other biological activities of these compounds, and their scarcity in nature, we initiated in vitro cultures of this species with the aim of developing a biotechnological process for obtaining taxol and related taxanes. In the current work, in a batch-type twophase culture of T. globosa, we evaluated the effect of cell immobilization and methyl jasmonate (MeJ) elicitation in two culture media containing different plant growth regulator combinations: 2,4-dichlorophenoxyacetic + benzylaminopurine (Treatment 1: T1) and picloram + kinetin (Treatment 2: T2). The productivity and excretion rate into the culture medium of baccatin III (12.79 lg L-1 d-1) (84 %), 10-deacetylbaccatin III (15 lg L-1 d-1) (0%), 10-deacetyltaxol (3.18 lg L-1 d-1) (63 %), and cephalomannine (49.27 lg L-1 d-1) (9 %) were increased by the effect of T1 in the free cell cultures elicited with MeJ. Cell immobilization in alginate beads did not improve the biotechnological production of these four taxanes. In contrast, the highest productivity of taxol (53 lg L-1 d-1) was achieved in MeJ-elicited free cells under T2 and cell immobilization in these conditions increased productivity by more than twofold (130.35 lg L-1 d-1).
Pierwsza strona wyników Pięć stron wyników wstecz Poprzednia strona wyników Strona / 1 Następna strona wyników Pięć stron wyników wprzód Ostatnia strona wyników
JavaScript jest wyłączony w Twojej przeglądarce internetowej. Włącz go, a następnie odśwież stronę, aby móc w pełni z niej korzystać.