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1

Role of IL-1beta in production of TNF-alpha and IL-6 by peripheral mononuclear cells following subarachnoid hemorrhage in rats

100%
Larysz-Brysz M., Jedrzejowska-Szypulka H., Olakowska E., Lewin-Kowalik J.
Acta Neurobiologiae Experimentalis
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2011
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tom 71
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nr S
Subarachnoid hemorrhage (SAH) develops when extravasated arterial blood enters subarachnoid space and mixes with cerebrospinal fluid. This leads to numerous pathologies, including increased synthesis of proinflammatory cytokines, like interleukin-1β (IL-1β). Through broken blood-brain barrier, IL-1β may stimulate peripheral leukocytes. These peripheral mononuclear cells (PMC) may be an additional source of other cytokines and migrating to the brain they can enhance or reduce the pathologies resulting from SAH. We examined the effect of neutralization of IL-1β on secretion of TNF-α and IL-6 by PMCs in adult rats following SAH. SAH was produced by injection of 150 μL of autologous arterial blood into cisterna magna. In 50% of animals, IL-1β activity was inhibited by intracerebroventricular administration of anti-rat IL-1β antibodies. Control group consisted of sham-operated rats. Ninety minutes or 24 hrs following surgery, blood samples were collected from the extraorbital plexus and centrifuged to separate leukocyte subpopulations. Isolated PMCs (monocytes and lymphocytes) were cultured for 24 hrs and TNF-α and IL-6 concentrations in the supernatants were assessed with ELISA. SAH led to the increase of production of both TNF-α and IL-6 by PMCs. Neutralization of IL-1β activity significantly reduced the concentration of both cytokines 90 min as well as 24 hrs after SAH. The results indicate an important role of IL-1β in the activation of peripheral mononuclear cells in the course of subarachnoid hemorrhage.
2

IL-1betta stimulates cytokine secretion by peripheral leukocytes following subarachnoid hemorrhage in rats.

88%
Jedrzejowska-Szypulka H., Woszczycka-Korczynska L., Olakowska E., Larysz-Brysz M., Lewin-Kowalik J.
Acta Neurobiologiae Experimentalis
|
2009
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tom 69
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nr 3
Much evidence indicates that pathologies observed after subarachnoid hemorrhage (SAH), including vasospasm and neurological defects, are the effects of immuno-infl ammatory processes accompanying the hemorrhage. Following SAH, CSF concentration of cytokines increase, what may change the cerebral perfusion and destroy brain–blood barrier. These cytokines are produced by activated leukocytes as well as hypoxic cells in the brain. The aim of this study was to examine the role of IL-1β in stimulation of peripheral leukocytes to increase secretion of cytokines after SAH. SAH was produced by injection of 150 μL of autologous arterial blood into cisterna magna. In 50% of animals, IL-1β activity was inhibited by intracerebroventricular administration of anti-rat IL-1β antibodies (SAH’ groups). Control group consisted of sham-operated rats. Ninety minutes or 24 h following surgery, blood samples were collected and subpopulations of mononuclear leukocytes were isolated and cultured for 24 h. Then, the concentration of TNF-α, ET-1 and IL-6 in supernatants was measured by means of ELISA. SAH resulted in signifi cant increase in TNF-α and ET-1, especially after 24 h, and no change in IL-6 concentration. Inhibition of IL1-β decreased the secretion of TNF-α and ET-1 and led to increase in IL-6. We found that peripheral leukocytes are an additional source of cytokines following SAH, and their activation depends on IL1-β produced after hemorrhage.
3

Bruceloza - deficyt zdrowia ludzkiego i zwierząt hodowlanych

75%
Olakowska E., Olakowski T.
Przegląd Mleczarski
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1959
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nr 01-02
4
Content available remote

Concentrations and activities of metalloproteinases 2 and 9 and their inhibitors (TIMPS) in chronic pancreatitis and pancreatic adenocarcinoma

51%
Lekstan A., Lampe P., Lewin-Kowalik J., Olakowski M., Jablonska B., Labuzek K., Jedrzejowska-Szypulka H., Olakowska E., Gorka D., Filip I., Dranka-Bojarowska D.
Journal of Physiology and Pharmacology
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2012
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tom 63
|
nr 6
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