Wyniki wyszukiwania

Sortuj według:

Ogranicz wyniki do:

Production and characterization of alpha-amylase from Aspergillus niger JGI 24 isolated in Bangalore

100%

Varalakshmi K. N., Kumudini B. S., Nandini B. N., Solomon J., Suhas R., Mahesh B., Kavitha A. P.

Polish Journal of Microbiology

2009

tom 58

nr 1

29-36

Five fungal isolates were screened for the production of α-amylase using both solid-state and submerged fermentations. The best amylase producer among them, Aspergillus niger JGI 24, was selected for enzyme production by solid-state fermentation (SSF) on wheat bran. Different carbon and nitrogen supplements were used to enhance enzyme production and maximum amount of enzyme was obtained when SSF was carried out with soluble starch and beef extract (1 % each) as supplements. Further attempts to enhance enzyme production by UV induced mutagenesis were carried out. Survival rate decreased with increase in duration of UV exposure. Partial purification of the enzyme using ammonium sulphate fractionation resulted in 1.49 fold increase in the enzyme activity. The enzyme showed a molecular weight of 43 kDa by SDS-PAGE. Metal ions Ca²⁺ and Co²⁺ increased the enzyme activity. The enzyme was optimally active at 30°C and pH 9.5.

Ograniczanie wyników

1 Polish Journal of Microbiology

1 Kavitha A. P.

1 Kumudini B. S.

1 Mahesh B.

1 Nandini B. N.

1 Solomon J.

1 Suhas R.

1 Varalakshmi K. N.

1 2009

Wyniki wyszukiwania

Production and characterization of alpha-amylase from Aspergillus niger JGI 24 isolated in Bangalore