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Four new species of earthworm species coEected from various parts of Hainan Island were found. The species belong to Amynthas and Metaphire and are characterized by having two pairs of spermathecae in 7/8-8/9. They are described here as Amynthas tlexuosus sp. nov., A. lucidus sp. nov., A. haikouensis sp. nov. in the aeruhinosus- group of species of the genus Amynthas, and Metaphire fortuita sp. nov. in the insularui-group of species of the genus Metaphire. Their anatomical and morphological characteristics are compared to similar species from Hainan Island, China mainland and South-East Asia.
The early ceratopsians Psittacosaurus and Protoceratops have provided important information on dinosaurian development because of abundant specimens of adults, subadults, juveniles, and even hatchlings. Here we present new data and methods for identifying key growth stages from bone histology. Previous studies on Psittacosaurus lujiatunensis from the Early Cretaceous Jehol Biota of China did not present in-depth analysis of growth patterns. Based on a histological study of 43 thin sections from 17 individuals of this species, we recognize four histological ontogenetic stages, i.e., hatchling, juvenile, sub-adult, and adult, but no fully-grown stage. We estimate life history and longevity from diaphyseal growth line counts and other features of histology. We show that P. lujiatunensis grew fast in early stages (hatchling, juvenile, and subadult), according to the density of vascular canals and the different type of bone tissue; the deposition of parallel fibred bone tissue in the outer cortex of the subadult stage indicates that growth rate was slowing down. We introduce a new graphical method to estimate the occurrence and volumes of vascular canals from thin sections more accurately than current two-dimensional approaches.
The Chinese government has made a commitment to achieve a 60-65% reduction of CO2 emissions by 2030 compared with that in 2005. Most provinces are assigned differentiated reduction tasks due to different natural resources endowment, energy consumption structure, and economic developments. Marginal abatement cost (MAC) supplies cost information on regional pollutant reduction processes and should be an important evaluation indicator of policies. In this study, we build a quadratic parametric directional distance function (DDF) to estimate provincial MAC of CO2 emissions in China during 2000-2015. Linear programming is used to solve the parameter estimation problem. Results are as follows: 1) LP method supplies efficient parameter estimation results and obtains 98.33% reliable MACs during the research period. 2) MAC keeps a growing trend for most provinces in 2000-2015. Especially when China enters the New Normal stage in 2012, this growing trend has been accelerated. These trends reveal that MAC gradually becomes a more important indicator to evaluate emission reduction measurements. 3) From a spatial distribution aspect, positive cluster feature has experienced such fluctuations as “apparent rise→significant decline→close to zero.” In this stage, their spatial cluster is close to random distribution state. Spatial heterogeneity turns to being enlarged, especially among provinces at higher MAC range. These evolutionary trends will have important influence on their carbon reduction measure implementing process. Eastern regions should turn more focus on low-carbon technology innovation to push their lowcarbon transformation. For middle and western regions, they should promote their production efficiencyand obtain more technology spillovers from eastern provinces in the future to stimulate their economic growth and low-carbon transformation.
It has hitherto been hard to prove that any association of juvenile dinosaurs represents original behaviour rather than sedimentary accumulation, and it has been hard also to determine the ages of such juveniles. A previously described specimen, which consists of an “adult” Psittacosaurus with 34 fully articulated juveniles, turns out to be a composite: the “adult” skull probably has been added, and in any case it is below breeding age. Other juvenile-only clusters have been reported, but the best examples that likely reflect behaviour rather than sedimentary accumulation are specimens from the Early Cretaceous Lujiatun beds in NE China, which were entombed beneath pyroclastic flow deposits. A remarkable juvenile-only cluster of Psittacosaurus shows clear evidence of different ages (five 2-year olds and one 3-year old) based on bone histological analysis. These juveniles may have associated together as a close-knit, mixed-age herd either for protection, to enhance their foraging, or as putative helpers at the parental nest.
Differentiated carbon dioxide emission reduction targets and optimizing industrial incentive policy is an important subject in China’s low-carbon economic transformation. With the application of the environmental input-output (EIO) method and the bi-proportional scaling updating schedule, the inter-industrial inputoutput tables in 2017 are forecasted and then carbon dioxide emissions of 30 industrial sectors are simulated in seven scenarios. Based on these results, conclusions are: 1. Twenty-five high carbon dioxide emission sectors among 30 national sectors are divided into three types. Five sectors are whole-process high carbon dioxide emission type, 18 are conductive type, and two are apparent high type. 2. Final demands keep the dominant role in pushing sectorial emissions growing, whether in total carbon dioxide emission intensity or emission quantities. Technical progress leads to emissions declines in intensity and quantity. Moreover, special energy-saving technical progress will gradually exceed universal technical progress in reduction effects. Whole-process high carbon sectors are the best selection to gain favorable incentive policies to promote carbon dioxide emissions reduction. Apparent high carbon sectors are in last place. 3. With incentive policies being improved, technical progress reduction effect is increasing. However, it is not enough to offset the driving effect from final demands growing in seven scenarios. More favorable incentives and investments should be allocated into high emission sectors, especially into the most sensitive ones.
Gene expression in embryogenesis of Brassica campestris L. is analyzed using cDNA-AFLP technique. The whole embryogenesis was divided into six embryonic stages. In total, 4,267 bands were obtained from embryogenesis of B. campestris L. A total of 216 transcriptderived fragments (TDFs), differentially expressed in six stages of embryogenesis, were selected for sequencing. By searching BLASTN and BLASTX, most of 204 TDFs were homologous to Arabidopsis thaliana and Brassica napus sequences, and the rest of the 12 TDFs did not match with other plants’ genes. As a result, 178 TDFs are matched with genes encoding either known or putative proteins in higher plants, and 26 TDFs are homologous to unknown genes. Besides, a selected subset of differentially expressed TDFs is confirmed by RT-PCR, and relative transcript abundance of TDFs was shown. Some embryo-specific genes are expressed in embryogenesis of B. campestris. Expression profiles of many TDFs show similarity to those of the relevant Arabidopsis genes. The potential roles of these genes in embryo development are discussed.
Chlorophyll a fluorescence transient can reveal the degree of thermal damage to plant photosynthetic organs and provide much physiological information about PSII fleetly. However, it is not clear how it reveals photosynthetic mechanisms of plants under decreasing soil moisture. In this paper, the photosynthesis characteristics and photosystem II (PSII) activities in leaves of Lonicera japonica under 11-level soil moisture were explored by gas-exchange analysis and JIP test. Both parameters of net photosynthetic rate (Pn) and water use efficiency (WUE) exhibited an increasing–decreasing trend with the decreasing relative soil water content (RSWC). At 29.7% ≤ RSWC≤ 79.6%, the main reason for Pn decrease was stomatal limitation; at 36.4% ≤ RSWC≤ 55.1%, both Pn and WUE were higher than the average. When RSWC was below 29.7%, Pn, Tr, and WUE significantly decreased. Meanwhile, a JIP-test analysis revealed a distinct K peak with decreases in the quantum yield and energy distribution ratio (ψo or φEo) and increases in the K phase (Wk) and J phase (Vj) at RSWC≤ 29.7%, indicating that severe drought (RSWC≤ 29.7%) damaged the leaf oxygen-evolving complex, caused the accumulation of Q −A in PSII electron acceptors, and, therefore, hindered the progression of the PSII electron transport chain. In addition, at RSWC≤ 29.7%, the absorption flux per CS (ABS/CSo) increased, while the phenomenological energy fluxes for TRo/CSo, ETo/CSo, RC/CSo decreased, indicating that the PSII reaction center was damaged and excessive light energy was accumulated. Therefore, the main reason for the decrease of photosynthetic efficiency was non-stomatal limitation at RSWC≤ 29.7%, and the maximum moisture deficit for the growth of Lonicera japonica is RSWC= 29.7%.
Abscisic acid (ABA) plays an important role in regulating photosynthesis under stress. To understand the differential function of exogenous ABA in the regulation of drought tolerance between two rice (Oryza sativa L.) genotypes, upland rice (UR, resistant to drought stress) and lowland rice (LR, susceptible to drought stress), photosynthetic parameters, chlorophyll fluorescence parameters, and the expression of chloroplast and ABA biosynthesisrelated genes were investigated under 15 % polyethylene glycol (PEG) and exogenous ABA (60 µM) treatments. In both rice lines, most of the photosynthetic parameters, chlorophyll fluorescence parameters, and chloroplast and ABA biosynthesis-related gene transcript levels were rapidly reduced by PEG stress, with the exception of up-regulated levels of OsPsbA, OsNCED3, OsNCED4, and OsZEP in LR and OsNCED3, OsNCED4, and OsZEP in UR. Moreover, a rapid stress-responsive regulation mechanism was found in UR according to the more rapid and strong up-regulation of three ABA biosynthesis-related genes in UR than in LR. Under PEG stress, exogenous ABA application significantly enhanced the recovery of the net photosynthetic rate (Pₙ), stomatal conductance (Gₛ), and transpiration rate (Tᵣ) in UR, with increased expression of OsPsbD1, OsPsbD2, OsNCED2, OsNCED3, OsNCED4, and OsNCED5. These data suggest a role for chloroplast and ABA biosynthesis-related genes in photosystem II (PSII) induction by exogenous ABA in the UR genetic background.
Cold-induced sweetening is one of the major factors limiting the quality of fried potato products. To understand the mechanisms of protein regulation for cold-induced sweetening in potato tubers, a comparative proteomic approach was used to analyse the differentially expressed proteins both during control (25°C, 30 days) and cold treatment (4°C, 30 days) using two-dimensional gel electrophoresis. Quantitative image analyses indicated that there were 25 protein spots with their intensities significantly altered more than twofold. Of these proteins, 9 were up-regulated, 13 were down-regulated, 2 were absent, and 1 was induced in the coldstored tubers. The MALDI-TOF/TOF MS analyses led to the identification of differentially expressed proteins that are involved in several processes and might work cooperatively to maintain metabolic homeostasis in tubers during low-temperature storage. The preponderance of metabolic proteins reflects the inhibition of starch re-synthesis and the accumulation of sugars in carbon fluxes, linking starch–sugar conversion. The respiration-related proteins suggest the transfer of respiratory activity from aerobic respiration to anaerobic respiration in the cold-stored tubers. The proteins associated with defence appear to protect the tuber cells from low-temperature stress. Some heat shock proteins that act as chaperones also displayed a differential expression pattern, suggesting a potentially important role in cold-stored tubers, although their exact contribution remains to be investigated. The proposed hypothetical model might explain the interaction of these differentially expressed proteins that are associated with cold-induced sweetening in tubers.
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