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The influence of cold pretreatment on androgenesis efficiency in isolated microspore culture of triticale (X Triticosecale Wittm.)

100%

Zur I., Godzisz I., Dubas E., Golemiec E., Wedzony M.

Zeszyty Problemowe Postępów Nauk Rolniczych

2006

tom 509

Stress was recognized as a major factor changing microspore development from a gamethophytic to a sporophytic pathway. Stress factors effectively inducing androgenesis in a wide spectrum of genotypes include high temperature, carbohydrate/nitrogen starvation and high-osmotic shocks. A low temperature treatment usually improve plant responsiveness, however in some cases, is not sufficient for embryogenesis initiation. Thus low temperature is not consider by some authors to be a true „stress factor”. In the experiment the effect of prolonged cold pretreatment of tillers on the effectiveness of androgenesis induction in isolated microspore cultures of hexaploid triticale (X Triticisecale Wittm.) was tested. In the study, two spring cultivars (cv. ‘Mieszko’ and cv. ‘Wanad’) varying in androgenic responsiveness were used. Prolongation of tillers cold pretreatment from 3 to 5 weeks, increased the number of microspores of 'Mieszko' isolated per spike from 5.8·10⁴ to 9.9·10⁴. It also promoted ELS development and its regeneration ability. ELS production increased more then 5-fold, when the number of regenerating ELS increased from 1.4% to 29%. At the same time, prolonged cold pretreatment of ‘Wanad’ tillers had no effect on the microspores yield received from one spike. Moreover, it reduced the number of produced ELS about 3-fold. Due to great variation among replicates the increase of ELS regeneration ability (from 2.8% to 24.5%) was not statistically significant. For both cultivars, the highest green plant regeneration percentage (24% and 8.2% for ‘Wanad’ and ‘Mieszko’, respectively) was noted after the longest cold pretreatment of tillers. However, the great variation among replicates made the comparison among treatments difficult for statistical analyses. Proper interpretation of the received results needs a further and more detailed examination.

The occurrence and comparative phenotypic characteristics of Staphylococcus spp. from healthy and diseased, household and shelter dogs, based on routine biochemical diagnostic methods

72%

Kasprowicz A., Bialecka A., Bialecka J., Godzisz I., Barabasz W., Jaworska O., Malachowa N., Miedzobrodzki J.

Polish Journal of Microbiology

2011

tom 60

nr 1

To determine the staphylococcal colonization pattern in healthy and diseased dogs, living in two particular environments, a number of microbiological samples were taken. Overall, twenty dogs, either healthy or with infected skin lesions, were examined. In each case bacterial swabs were collected from the nasal mucosa, ear, perineum, lumbo-sacralis triangle, and from the infection sites if such were present. A total number of 104 isolates representing different staphylococcal species were isolated and identified using routine biochemical methods applied in diagnostic laboratories. Among 17 isolated staphylococcal species, Staphylococcus intermedius was the most common species isolated from both healthy or diseased dogs living either in animal shelter or household environments. The pattern of Staphylococcus sp. colonization differs considerably for animals living in the two tested habitats. In particular, S. aureus MRSA and MSSA isolates were detected only in infected skin lesion samples from animals that dwelled in the animal shelter. As could be expected, S. intermedius was found to be a predominant causative agent in canine skin infections. In our study, we demonstrated that S. intermedius in its carrier-state, inhabits mainly the mucosal membrane of the nasal vestibule. It was also found in the samples taken from the skin, the lumbo-sacralis triangle and perineum, but was rarely isolated from the ears.

Ograniczanie wyników

1 Polish Journal of Microbiology

1 Zeszyty Problemowe Postępów Nauk Rolniczych

2 Godzisz I.

1 Barabasz W.

1 Bialecka A.

1 Bialecka J.

1 Dubas E.

1 Golemiec E.

1 Jaworska O.

1 Kasprowicz A.

1 Malachowa N.

1 Miedzobrodzki J.

1 Wedzony M.

1 Zur I.

1 2011

1 2006

Wyniki wyszukiwania

The influence of cold pretreatment on androgenesis efficiency in isolated microspore culture of triticale (X Triticosecale Wittm.)

The occurrence and comparative phenotypic characteristics of Staphylococcus spp. from healthy and diseased, household and shelter dogs, based on routine biochemical diagnostic methods