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Puccinellia distans is a non-agricultural halophytic grass that has become another host plant for Epichloe typhina, hitherto not reported from Poland. In 1992 we noticed the first symptoms of choke disease in a single population of P. distans in central Poland. Since then we have observed choke disease in 5 populations of P. distans only in man-made habitats. These habitats are strongly anthropogenically salinated but they exhibit the pattern of species composition characteristic of natural salines. In this paper we test the hypothesis that the level of salinity affects the infection of P. distans by the fungus E. typhina. Seven plots were established in the field and each plot was divided into 25 subplots. Within each plot the level of infection in a spring generation of shoots was negatively correlated with salinity (common regression within the plots, beta = -0.674, df = 117, p < 0.001). Negative correlation was also found in an autumn generation within the plots (beta = -0.682, df = 94, p < 0.001) after excluding plot P in which the frequency of infected individuals was the lowest and equal only to 0.05. The proportion of individuals infected by the endophytic stage of the fungus in the populations was assessed using diagnostic polymerase chain reaction. The greatest percentage (98.3%) of infected individuals was found in the population growing in the habitat of the lowest salinity. The high salinity reduces the chance of P. distans to become infected, but may promote the stroma formation of E. typhina twice in the season. Disease expression in autumn clearly represents a misadaptation which could be explained by the fact that the species interactions described here appeared relatively recently as a result of human activity. This hypothesis requires further experimental verification.
In this paper we present a hypothesis about the loss of organisms associated with hosts in small populations. Data for fungi specific to arcto-alpine plants are presented. We found that the number of fungal species on plants is directly proportional to the size of the host population. This conforms to MacArthur and Wilson's theory of island biogeography. Once the host population is below a critical size, some fungal species are lost. We term this process 'symbiotic drift.'
Behavioural activity of Clethrionomys glareolus (Schreber, 1780) is modified by social factors. Interaction between sexually nonexperienced (NExp) and experienced (Exp) animals was investigated. Males and females were tested in pairs in the male home cage during 10 min encounters. Aggressive and nonaggressive behaviour was recorded, and ultrasonic vocalization was monitored by a QMC ultrasound detector. The obtained results indicate that total activity of Exp females was significantly higher than NExp females (p < 0.05). Only females exhibited aggressive behaviour toward males, but there was no difference in aggressive activity related to sexual experience. Total activity of Exp males was higher than that of NExp ones (p < 0.05). Our previous results indicate that in adult bank voles only males produce ultrasounds. In these experiments significantly more calls was recorded when Exp males were tested and compared with NExp males (p < 0.05). This study provides additional information about interaction between breeding and non-breeding animals in a bank vole popu­lation.
Housing females in groups prolongs the dioestrous phases in cycling laboratory mice Mus rnusculus Linnaeus, 1758. Olfactory signals play an important role in interaction between females, and the vomeronasal system is involved in mediation of hormonal responses to pheromones. Present results show that removal of the vomero­nasal system (VNX), or bulbectomy (OBX), leads to in decreased numbers of oestrous mice in comparison to non-operated (NON) or sham-operated (SHAM-OBX) singly reared females. Oestrous cyclicity was also affected by social factors. Cohabitation of NON, VNX, OBX or SHAM-OBX females with adult females results in decreased oestrous frequency in comparison to females reared alone. Moreover, VNX females exposed to male bedding inhibited oestrus in comparison to nonexposed VNX animals. The results provide evidence that different external stimuli evoke hormonal responses which are manifested in inhibition of oestrous cycle in female mice.
The size and reproduction ability of the three field populations of Carex secalina Willd. ex Wahlenb. have been assessed. In the parallel garden study selected traits from the life history of the species have been studied, such as age at first reproduction, fertility, the size of seeds, their germination ability and size of seedlings. The populations of C. secalina discovered in Poland in 2000 are characterised by small abundance and small area. All individuals from the three populations in the garden produced generative shoots in the third year of life. Statistically significant differences between the populations were found in the production of shoots with unisexual spikes and bisexual ones, the latter had not been reported in the hitherto literature on the species. The seeds started germinating after a 6-months rest. The first seedlings were observed in the first decade of May. The largest seedlings were noted in the population producing the smallest seeds. The results contribute to explaining the renewal of the populations of this species in the field.
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