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1

Spumawirusy - zagadkowe retrowirusy

100%
Materniak M., Kuzmak J.
Medycyna Weterynaryjna
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2006
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tom 62
|
nr 07
744-748
Spumaviruses belong to the family of Retroviridae, genus Spumavirus. They are also commonly known as foamy viruses or syncytial viruses because of inducing the spectacular cytopathic effect in tissue culture, which produces vacuolated “foamy” forms and the appearance of typical syncytia. Foamy viruses are widespread and have been isolated from many mammals, such as primates, pets and livestock animals. The resultant infection is persistent and infected individuals develop a strong antibody response. Although these viruses cause a strong cytophatic effect in cell cultures, they have never been linked to particular symptoms or pathalogical developments. During the last few years the research on spumaviruses has increased, mainly due to their possible zoonotic potential and thus a great deal of activities connected with the development of diagnostic assays and pathogenicity studies were carried out. This paper reviews the present stage of knowledge on spumaviruses found in humans and animals.
2

Detection of bovine foamy virus in milk and saliva of BFV seropositive cattle

81%
Materniak M., Sieradzki Z., Kuzmak J.
Bulletin of the Veterinary Institute in Puławy
|
2010
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tom 54
|
nr 4
The aim of this study was to demonstrate the oceurrence of bovine foamy virus (BFV) and BFV DNA in peripheral blood leukocytes (PBLs), milk cells, and saliva of cattle serologically positive to BFV. The virus was detected by co-cultivation technique with canine thymus cells (Cf2Th) and viral DNA was quantified by the real-time PCR. Out of co-cultures from 23 cattle, BFV was found in 19, 13, and 15 samples of PBLs. milk cells and saliva, respectively, while BFV-DNA was confirmed in DNA extracted from 14 PBLs and 8 milk cells samples. All 23 saliva samples were negative. The number of BFV DNA copies in milk cells was in average over 2.5 times lower than in PBLs. The presence of BFV and viral DNA in milk and saliva samples is discussed in the context of virus spread and possible human exposure to BFV through food products of cattle origin.
3

Reactivity of serum from cattle suspected of bovine leukaemia virus infection to peptides representing mutated epitopes on envelope glycoprotein gp51

80%
Rola M., Materniak M., Kuzmak J.
Bulletin of the Veterinary Institute in Puławy
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2008
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tom 52
|
nr 3
The ELISA technique with synthetic peptides as an antigen was developed to analyse the reactivity of bovine serum directed to mutated epitopes on envelope glycoptrotein gp51 of bovine leukaemia virus (BLV). The peptides represented normal and mutated G, H, and GG epitopes deduced from wild type BLV and its genetic variants, respectively. When 436 sera identified as seronegative by commercially available ELISA were tested by newly developed peptide ELISA, 14 samples revealed the highest reactivity. Out of them, reactivity to the mutated epitope GG was found in eight samples, while five and two samples reacted to epitopes G and H, respectively. One serum showed the reactivity to two peptides. Sera, which reacted with the epitope G, were found mostly in animals from one herd, while the distribution of sera that reacted to epitopes GG and H were less restricted to the particular herds. It has been concluded that sera showing strong reactivity to mutated peptides could represent animals infected with BLV variants, which escape antibody detection.
4

Isolation and partial characterization of bovine foamy virus from Polish cattle

80%
Materniak M., Bicka L., Kuzmak J.
Polish Journal of Veterinary Sciences
|
2006
|
tom 09
|
nr 4
The first isolation and partial characterization of bovine foamy virus (BFV), also known as bovine syncytial virus, in Poland is described. This virus was isolated by co-cultivation of peripheral blood leukocytes from infected cattle with permissive Cf2Th cells. The new isolate, called BFV100 was identified using several techniques: electron microscopy, western blotting, PCR and sequencing of a part of the gag and pol/env genes. Based on syncytia induction, antigenic determinants, primer binding sites and sequence analysis, it can be concluded that isolate BFV100 is bovine foamy virus and is related to the known American and German BFV isolates by sequence homology and antigenic relatedness.
5

Isolation and characterization of caprine arthritis encephalitis virus in goats from Poland

71%
Kaba J., Rola M., Materniak M., Kuzmak J., Nowicki M.
Polish Journal of Veterinary Sciences
|
2009
|
tom 12
|
nr 2
183-188
The caprine arthritis-encephalitis virus (CAEV) was isolated from monocyte-derived macrophages (M/M), but not from PBMC of seropositive goats by co-cultivation with goat synovial membrane cells. Out of eight M/M co-cultures, CAEV was evidenced by the syncytia formation and presence of proviral DNA in two and four cultures, respectively. Two virus isolates from co-cultures showing cytopathic effects were further confirmed as CAEV by western blotting, PCR, and sequence analysis. The nucleotide sequence of gag gene showed 92.0% and 90.3% homology to the prototype CAEV-Co strain. Supernatants harvested from these cultures induced syncytia when cultured with uninfected cells and the resultant titer was 1035 and 1025 TCID50 per ml. New CAEV isolates are suitable candidates for further analysis of their genetic and biological properties.
6

Evidence for maedi-visna virus in milk epithelial cells of naturally infected ewes

71%
Materniak M., Kubis P., Rola-Luszczak M., Olech M., Kuzmak J.
Bulletin of the Veterinary Institute in Puławy
|
2011
|
tom 55
|
nr 4
Epithelial cells (MEC) were isolated from milk of four maedi-visna virus (MVV) infected and two uninfected ewes, cultured in vitro, and their epithelial nature was confirmed by immunocytochemistry with cytokeratin monoclonal antibodies. PCR analysis on MEC DNA confirmed the presence of proviral DNA in half cultures from infected animals. Expression of MVV proteins was shown both by immunocytochemistry using anti-p27 mAbs and by western blot with hyperimmune serum. Supernatants of MEC from infected ewes were showed to contain infectious viruses when assayed on the indicator goat synovial membrane cells (GSM). Furthermore, p27 protein was detected in supernatants of infected MEC suggesting active production of viral particles. No cytopathic effect was noted in MEC cultures from all infected animals; however, cocultivation of MEC with GSM promoted CPE development. These results suggested that MEC of ewes naturally infected with MW may play an important role in sustaining virus transmission through the milk.
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