Wyniki wyszukiwania

1

Molecular identification, sequence analyses, tissue expression profile of a novel cattle gene-GBE1 in Yunnan cattle

100%

Liu G. Y.

Journal of Animal and Feed Sciences

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2008

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tom 17

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nr 4

2

A novel HADHA gene differentially expressed in muscle and other tissues from black-boned vs. ordinary sheep

100%

Liu G. Y.

Animal Science Papers and Reports

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2009

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tom 27

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nr 2

127-137

The mRNA differential display technique was applied to investigate the differences in gene expression in the muscle tissue between black-boned and ordinary (“normal”) sheep. The gene that was differentially expressed was identified through semi-quantitative RT-PCR, and its complete cDNA sequence was then obtained using the rapid amplification of cDNA ends (RACE) method.The nucleotide sequence of the gene was not found homologous with any sheep gene described so far. Sequence prediction analysis revealed that the open reading frame of the gene encodes a protein of 763 amino acids, highly homologous with the hydroxyacyl-coenzyme A dehydrogenase/3-ketoacyl-coenzyme A (HADHA) of bovine (98%), pig (90%), sumatran orangutan (87%), human (87%), chimpanzee (87%), mouse (83%) and rat (82%) – so that it can be defined as sheep HADHA gene. This novel sheep gene was finally assigned to GeneID: 100192316. Phylogenetic tree analysis revealed that the sheep HADHA gene is genetically closely related to the HADHA gene of cattle. Tissue expression analysis indicated that the sheep HADHA is also differentially expressed In different tissues of black-boned sheep. The present study established the primary foundation for further research on the HADHA gene in sheep.

3

Isolation, sequence identification, and tissue expression profile of 3 novel porcine genes: NCF2, BCKDHB and BCKDHA

100%

Liu G. Y.

Journal of Applied Genetics

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2009

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tom 50

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nr 1

47-50

The complete coding sequences of porcine genes NCF2, BCKDHB and BCKDHA were amplified by using reverse transcriptase polymerase chain reaction (RT-PCR), basing on the conserved coding sequence information of humans or other mammals. These 3 novel porcine genes were then assigned GeneIDs: 100142665, 100142669 and 100142666. The phylogenetic tree analysis revealed that the porcine NCF2, BCKDHB and BCKDHA all are most closely related to the bovine NCF2, BCKDHB and BCKDHA. Tissue expression profile analysis revealed that porcine NCF2, BCKDHB and BCKDHA genes were differentially expressed in tissues, including skeletal muscle, the heart, liver, fat, kidney, lung, small and large intestine.

4

A novel porcine mRNA differentially expressed in the Longissimus muscle tissues from Meishan and Large White pigs are associated with carcass traits and meat quality

63%

Liu G. Y., Xiong Y. Z.

Journal of Animal and Feed Sciences

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2008

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tom 17

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nr 4

5

Molecular characterization and tissue transcription profile analyses of three novel pig genes - NDP, CLN3 and NCF1

45%

Wang G. P., He Y. Y., Hu S. F., Liu W., Liu G. Y.

Journal of Animal and Feed Sciences

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2009

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tom 18

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nr 1

Ograniczanie wyników

3 Journal of Animal and Feed Sciences

1 Animal Science Papers and Reports

1 Journal of Applied Genetics

5 Liu G. Y.

1 He Y. Y.

1 Hu S. F.

1 Liu W.

1 Wang G. P.

1 Xiong Y. Z.

3 2009

2 2008

Molecular identification, sequence analyses, tissue expression profile of a novel cattle gene-GBE1 in Yunnan cattle

A novel HADHA gene differentially expressed in muscle and other tissues from black-boned vs. ordinary sheep

Isolation, sequence identification, and tissue expression profile of 3 novel porcine genes: NCF2, BCKDHB and BCKDHA

A novel porcine mRNA differentially expressed in the Longissimus muscle tissues from Meishan and Large White pigs are associated with carcass traits and meat quality

Molecular characterization and tissue transcription profile analyses of three novel pig genes - NDP, CLN3 and NCF1