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As a traffic engineering project across straits or gulfs, subsea tunnel is one of the oceaneering with great construction difficulties, and the key to the success of subsea tunnel engineering lies in timely and accurate assessment of the structure safety of subsea tunnel engineering construction and life-cycle. Xiang’an Xiamen subsea tunnel is China’s first subsea tunnel which crosses complex formation conditions, engineering accidents such as collapse, sudden inflow of water or mud might occur during tunnel construction and operation. Therefore, the concept of subsea tunnel life-cycle monitoring is proposed aiming at the particularity of subsea tunnels. The variation forms of subsea tunnel mainly include large deformation, collapse, primary support cracking, water leakage, water pressure increase, steel arch corrosion, concrete corrosion, longitudinal differential settlement, etc., and classification of the life-cycle safety monitoring levels of the subsea tunnel is conducted based on risk assessment theory and risk level management benchmark to determine the possible variation forms in the monitoring level segments. The research results will provide reference for the subsea tunnel life-cycle monitoring, disaster warning as well as risk management under construction or to be built at home and abroad
Our previous study has shown that MAIGO2 (MAG2) is a subunit of the Golgi/endoplasmic reticulum (ER) multi-subunit tethering complex, and is required for tolerance to general osmotic stresses and abscisic acid and response to ER stress during seed germination and early growth. MAG2 is crucial for multi-environmental stress responses. To verify this hypothesis, the response of mag2 mutants to gibberellic acid (GA), sugar, and heat shock was described in this study. The mag2 mutants showed defects during seed germination and early seedling development under treatments with the GA biosynthesis inhibitor paclobutrazol, sucrose, and glucose. MAG2 is also essential for basal thermotolerance. However, the MAG2 homolog (MAG2L) is not necessary for these responses. MAG2 is an important regulator in the response to multi-environmental stimuli, supposedly through its roles in Golgi/ER retrograde trafficking and ER stress response.
An important behavioural adaptation for animal species with variable or unpredictable food availability is storing food. Food availability for large field mouseApodemus peninsulae (Thomas, 1907) is not reliable. We conducted a series of tests with the large field mouse to determine food hoarding behaviour, response when their hoarded food was removed, and whether perishable foods were treated different than non perishable foods. The study was conducted in four semi-natural enclosures (4 × 3 × 1 m), established on the Donglingshan Mountain near Beijing, China. Thirteen large field mice were placed in enclosures and offered wild apricotPrunus armeniaca seeds and Liaodong oakQuercus liaotungensis acorns. Our results indicated that although large field mice hoarded seeds in larder and scatter patterns, they more frequently exhibited larder hoarding. Liadong oak acorns were generally consumed near the feeder, whereas apricot seeds were more frequently transported to the nest box. Only apricot seeds were scattered among hoard sites. When seeds were removed from hoarding sites the mice responded by taking increased amounts of seeds to their nest for larder and scatter hoarding. Hoarding sites were not randomly distributed throughout the enclosure.
Due to the great danger of the collision of oil tankers, lots of research on the collision of oil tankers has been carried out. But, at present, the research on the collision of oil tankers mainly focuses on the loading condition of the struck ship, ignores the impact on the loading condition of the striking ship. However, during the actual oil tanker collision, the striking ship is generally in the state of loading. Therefore, it is necessary to carry out the analysis of the impact of the loading condition of the striking ship on the collision damage of the oil tanker. In this paper, the effect of striking ship with loading on the impact performance of the side structure during the collision of the cargo double hull oil tanker has been investigated. The ship collision model was established by using the finite element software ANSYS/LS-DYNA which is based on 7000 tons of double hull oil tankers. Based on the analysis of the collision force, impact of striking speed changes, impact of striking deep changes and structural energy absorption during the collision process, the influence of the striking ship with loading on the damage mechanism and the impact performance of the double shell oil ship side structure was expounded. The results show that the influence of the striking ship with loading can be great to the damage to side hull during the research of the collision performance of the oil tanker
Background: Mishandling of intracellular chloride (Cl−) concentration ([Cl−]i) in cerebrovascular smooth muscle cells is implicated in several pathological processes, including hyperplasia and remodeling. We investigated the effects of ClC-2-mediated Cl− efflux on the proliferation of human brain vascular smooth muscle cells (HBVSMCs) induced by angiotensin II (AngII). Methods: Cell proliferation and motility were determined using the CCK-8, bromodeoxyuridine staining, wound healing and invasion assays. ClC-2, PCNA, Ki67, survivin and cyclin D1 expression, and β-catenin and GSK-3β phosphorylation were examined using western blotting. Histological analyses were performed using hematoxylin and eosin staining and α-SMA staining. Results: Our results showed that AngII-induced HBVSMC proliferation was accompanied by a decrease in [Cl− ]i and an increase in ClC-2 expression. Inhibition of ClC-2 by siRNA prevented AngII from inducing the efflux of Cl− . AngII-induced HBVSMC proliferation, migration and invasion were significantly attenuated by ClC-2 downregulation. The inhibitory effects of ClC-2 knockout on HBVSMC proliferation and motility were associated with inactivation of the Wnt/β-catenin signaling pathway, as evidenced by inhibition of β-catenin phosphorylation and nuclear translocation, and decrease of GSK-3β phosphorylation and survivin and cyclin D1 expression. Recombinant Wnt3a treatment markedly reversed the effect of ClC-2 knockdown on HBVSMC viability. An in vivo study revealed that knockdown of ClC-2 with shRNA adenovirus ameliorated basilar artery remodeling by inhibiting Wnt/β-catenin signaling in AngII-treated mice. Conclusion: This study demonstrates that blocking ClC-2-mediated Cl− efflux inhibits AngII-induced cerebrovascular smooth muscle cell proliferation and migration by inhibiting the Wnt/β-catenin pathway. Our data indicate that downregulation of ClC-2 may be a viable strategy in the prevention of hyperplasia and remodeling of cerebrovascular smooth muscle cells.
As energy crises and environmental pollution become increasingly prominent, people are beginning to explore the ocean to exploit its renewable energy. Based on hydrodynamic principles, an analytical model for unstable hydrofoil motion has been developed. The software ANSYS Fluent was employed to perform a simulation of hydrofoil motion, and the effect of hydrofoil motion on the surrounding flow field was analyzed. The hydrodynamic characteristics and energy-capturing efficiency of the hydrofoil were obtained, and the influence of complex flows on hydrofoil hydrodynamics was elucidated. The energy dissipation mechanism during hydrofoil motion was characterized. The results indicate that the vortex generation and shedding inevitably dissipates part of the tidal energy captured by the hydrofoil, which leads to abrupt changes in hydrofoil hydrodynamics and reduces energy-capturing efficiency. When the frequency of the abrupt hydrodynamics change matches the hydrofoil natural frequency, it may result in hydrofoil resonance and damage. Also, it is observed that larger pitch amplitude leads to larger optimalreduced frequency corresponding to the peak power cycle-averaged coefficient. The results also provide a theoretical guide on how to improve hydrofoil energy-capturing efficiency of the power generation system with control valves and extend hydrofoil life.
Based on specimen examination and previous records, we provide a checklist including 146 species from 51 genera and 10 tribes of the weevil subfamily Ceutorhynchinae in China. Of these species, the following 18 are reported in China for the first time: Amalus scortillum (Herbst, 1795), Ceutorhynchus filiae Dalla Torre, 1922, Dieckmannius ingensColonnelli, 1993, Dieckmannius sexnotatus (A. Schultze, 1899), Nedyus quadrimaculatus (Linnaeus, 1758), Zacladus stierlini (A. Schultze, 1902), Auleutes epilobii (Paykull, 1800), Hypurus bertrandi (Perris, 1852), Hypurus portulacae (Marshall, 1916), Neoplatygaster venusta (Faust, 1885), Belonnotus tenuirostris (Marshall, 1917), Mecysmoderes fulvus (Roelofs, 1875), Xenysmoderes stylicornis (Marshall, 1934), Mononychus ireos (Pallas, 1773), Rhinoncus jakovlevi Faust, 1893, Rutidosoma globulus (Herbst, 1795), Rutidosoma graminosum (Gistel, 1857) and Rutidosoma koreanumKorotyaev & Hong, 2004.
In order to assess the spatial distribution and the potential pollution risk of nickel (Ni) and vanadium (V) in Shuangtaizi Estuary in China, the water and the surface sediments were collected to determine V and Ni content in specified sites. The data indicated that the estuary was contaminated by Ni, while V content was low. Total concentrations of V in river water ranged from 0.212 to 0.374 μg/L, and in surface and bottom seawater from 0.214 to 0.559 μg/L and 0.231 to 0.684 μg/L, respectively. V content in river sediments ranged from 0.093 to 0.119 μg/g and in ocean sediments were 0.032 to 0.123 μg/g. Total Ni concentrations in river waters ranged from 23.667 to 118.667 μg/L, and in surface and bottom seawaters were 32.000 to 90.323 μg/L and 42.000 to 100.333 μg/L, respectively. Ni content in sediments of river ranged from 15.303 to 21.732 μg/g and in sediments of ocean were 11.197 to 21.107 μg/g. Ni content was higher than that of V. An ecological risk assessment study shows that Ni has potential ecological risk in water and no risk in sediment. V has no potential ecological risk in Shuangtaizi Estuary. V and Ni concentrations increased from the inner estuary to the outer estuary. The anthropogenic emissions from land are the main sources of Ni and V in the study area.
Yield loss due to insufficient potassium fertilizer supply has been well documented; however, the information about the negative effect of potassium deficiency on crop yield caused by ecophysiological determinants is not enough. A field experiment with three K treatments (severe K deficiency treatment, K₁; moderate K deficiency treatment, K₂; and sufficient K supply treatment, K₃) was conducted to (1) assess the effects of potassium deficiency on green leaf area index (GLAI) reduction; (2) quantify the contributions of single leaf area, leaf senescence, and leaf appearance to GLAI reduction under potassium deficiency; (3) reveal the changes in the contributions of accumulated radiation interception (RIacc) and radiation-use efficiency (RUE) to above-ground biomass (AM) decrease of oilseed rape under different K supplies. GLAI was restrained due to potassium deficiency, with a reduction ranging from 10.6 to 45.4%. The reduced single leaf area and accelerated leaf senescence caused by potassium starvation accounted for 5.9–23.7% and 2.4–29.0% reduction in GLAI, but delayed leaf appearance rate contributed little. The RIacc during the seedling stage in the K₁, K₂, and K₃ treatments was 101.2, 110.7, and 120.0 MJ m⁻² , respectively, and the RUE in the K₁, K₂, and K₃ treatments was 1.03, 2.22, and 2.98 g MJ⁻¹ , respectively, which caused a 61.7% and 48.2% reduction of the final harvested AM in the K₁ and K₂ treatments compared with the K₃ treatment. When AM reduction was less than 24.8%, RIacc was the main determining factor; however, it transferred to RUE when biomass decreased more. In conclusion, GLAI decreased due to potassium starvation was mainly caused by the reduced single leaf area and accelerated leaf senescence, and the relative contribution of RIacc and RUE to AM decline was related to the degree of potassium deficiency.
A new troodontid dinosaur, Xixiasaurus henanensis gen. et sp. nov., from the Upper Cretaceous Majiacun Formation of the Xixia Basin, Henan Province, is erected, based on a partial skull. It is characterized by bearing 22 maxillary teeth, a distinct opening on the lateral surface of the base of nasal process of the premaxilla, the rostral end of the upper jaw forming a tapered U−shape, and the mandibular symphyseal region slightly inflected medially. Xixiasaurus is most closely related to the Mongolian Byronosaurus among troodontids. Byronosaurus, Urbacodon, and Xixiasaurus may form a new clade, suggesting an endemic radiation of troodontids across Asia, including multiple taxa without dental serrations. The discovery of Xixiasaurus in the Xixia Basin may imply that the Xixiasaurus−bearing Majiacun Formation is Campanian in age.
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