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The aim of the authors’ study was to determine the effects of various concentrations of a glycerol-containing preparation on the dynamics of changes in individual parameters of rumen fluid and energetic profile in dairy cows. The presented results suggest a potential positive effect of glycerol as an energetic supplement to dairy cows. A number of studies have showed that the positive effect of glycerol is influenced by the administered doses, time of feeding and the form of administered glycerol. In this experiment a significant positive effect of glycerol was observed only in the third experimental group that was administered the highest dose of the investigated preparation and was reflected in pH (P < 0.05), acetic acid (P < 0.05), butyric acid (P < 0.0) and BHB (P < 0.01). The recorded moderate antilipolytic and glucoplastic effects of glycerol and the buffering capacity of the powder vehicle used (zeolite) on the health of dairy cows needs to be further verified in a long- -term experiment with the highest dose of glycerol.
The aim of the study was to examine the prevalence of antibodies against E. intestinalis in the blood serum of swine by the ELISA test and the possible effects of different states of the reproductive period on the occurrence of antibodies. The presence of specific anti-E. intestinalis antibodies was detected in 30 (56.6%) of 53 sows from the total count of examined sera. Each of the positive sera was reacted by the titre 1 : 200. Serological positivity to E. intestinalis was detected in 10 (58.8%) sows one week after birth of 17, in 6 (50%) sows one week before the birth of 12, in 7 (58.3%) sows one week after the weaning of 12 and in 7 (58.3%) sows one month after the birth of 12. The study did not detect any meaningful effect in the different state of the reproductive period of sows on the occurrence of antibodies.
Parasitic diseases of livestock together with poor welfare conditions can negatively affect the health status and production of small ruminants. Protozoan parasites and tick-borne infectious agents are common threat of livestock including small ruminants mostly during the pasture season. Therefore the priority of the study was to analyse the circulation and presence of two protozoan parasites Toxoplasma gondii and Neospora caninum as well as tick-transmitted bacterium Anaplasma phagocytophilum in one selected goat farm in Eastern Slovakia. Throughout a three-year study period we have repeatedly screened the sera and blood of goats and dogs from monitored farm. In total, 343 blood serum samples from 116 goats were examined by ELISA. The mean seropositivity for T. gondii was 56.9% (66/116, CI (95%) = 48–66.0) and 15.5% (18/116, CI (95%) = 9.3–22.7) for N. caninum. The permanent occurrence of anti-Toxoplasma and anti-Neospora antibodies was detected in repeatedly examined goats during the whole monitored period. The presence of both parasites in the flock was analysed by PCR. DNA of T. gondii was confirmed in 12 out of 25 Toxoplasma-seropositive goats and N. caninum in 14 samples out of 18 Neospora-seropositive animals; four goats were co-infected with both pathogens. The risk of endogenous transmission of both parasites was pursued by examination of 41 kid’s sera, where seropositivity for toxoplasmosis was 31.7% and for neosporosis 14.6%. In dogs 61.1% seropositivity for T. gondii and 38.9% for N. caninum was found, however, their faeces were negative for coccidian oocysts. Eight out of 108 tested animals were infected with A. phagocytophilum, the causative agent of tick-borne fever. Seven of them were simultaneously infected with T. gondii and A. phagocytophilum, out of which four goats were concurrently infected with all three pathogens.
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