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Detection and copy number estimation of the transgenic nucleotide sequences in an unknown GM event of Oryza sativa

100%

Sajjad A. M., Bashir T., Saeed S., Ahmad E.

Acta Agrobotanica

2016

tom 69

nr 4

The present study was designed to establish a qualitative detection method based on conventional and real time PCR assay to screen the commonly grown rice varieties for the presence of the cry1Ac gene. The detection of genetically modified rice in the screening process would necessitate accurate assay development and precise qualitative PCR tests complying with established procedures for the detection and characterization of transgenes in food grains. Such assay would not only enable the monitoring of transgene flow in local agricultural environment but also the characterization of different plant species produced with this transgene and its regulatory components. Thus, a reliable and quick screening assay was established for the qualitative detection of the transgene along with the promoter and selectable marker gene in genetically modified rice. By conventional PCR, a fragment of 215 bp was amplified with gene specific primers of cry1Ac. Primers for other transgenes such as gna and bar were also employed; however, no amplification was detected. The presence of the p35s, sps, and nptII genes was confirmed by qualitative real-time PCR. The specificity of the respective PCR products was checked through melt peak curve analysis. Sharp and precise melting temperatures indicated the presence of a single kind of PCR product in correspondence to each of the primers used. Moreover, the copy number of cry1Ac was estimated by ΔΔCT method. It is proposed that the primer sets and experimental conditions used in this study will be sufficient to meet the requirements for molecular detection and characterization of the cry1Ac transgene and affiliated sequences in sorting out conventional rice varieties from the ones which are genetically modified. It will also help to monitor the ecological flow of these transgenes and other biosafety factors.

Integrating aspects of ecology and predictive modelling: implications for the conservation of the leopard cat (Prionailurus bengalensis) in the Eastern Himalaya

88%

Bashir T., Bhattacharya T., Poudyal K., Sathyakumar S., Qureshi Q.

Acta Theriologica

2014

tom 59

nr 1

An understanding of species ecology is vital for effective conservation, particularly if the species forms an important constituent of the lesser mammal guild and regulates small mammal and bird populations. As the ecological role of the leopard cat (Prionailurus bengalensis) in the intricate eastern Himalayan habitats is not known, we assessed the site occupancy, detection probability and activity pattern of leopard cats in Khangchendzonga Biosphere Reserve, India, based on sign surveys and camera trapping. The estimated site occupancy was 0.352 ± 0.061 and detection probability was 0.143 ± 0.0484. Occupancy modelling indicated low elevation, high rodent abundance and tree cover as best predictors for the occupancy of leopard cat. Diet based on analysed scats revealed murids as the most dominant prey (89.2 %). Information based on photographic captures indicated that the leopard cat exhibited a nocturnal activity pattern (peak activity between 0200–0300 hours), which coincided with its principal prey (revealed through diet analysis), but mainly contradicted with other sympatric competitors, hence indicating a temporal partitioning of resources among them. Ecological niche factor analysis indicated that the leopard cat exhibits high global marginality (1.32) and low global tolerance (0.275). The habitat suitability map for leopard cats showed majority of the habitat as unsuitable (1,959.44 km2) and predicted only 164.54 km2 areas of lower temperate forests as moderate to highly suitable. As highly suitable habitats of the leopard cat are in close proximity to villages, conflict issues are a major threat and therefore need to be addressed in conservation program for this felid.

Ograniczanie wyników

1 Acta Agrobotanica

1 Acta Theriologica

2 Bashir T.

1 Ahmad E.

1 Bhattacharya T.

1 Poudyal K.

1 Qureshi Q.

1 Saeed S.

1 Sajjad A. M.

1 Sathyakumar S.

1 2016

1 2014

Wyniki wyszukiwania

Detection and copy number estimation of the transgenic nucleotide sequences in an unknown GM event of Oryza sativa

Integrating aspects of ecology and predictive modelling: implications for the conservation of the leopard cat (Prionailurus bengalensis) in the Eastern Himalaya