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1

PCR detection of Scopulariopsis brevicaulis

100%
Kordalewska M., Brillowska-Dabrowska A.
Polish Journal of Microbiology
|
2015
|
tom 64
|
nr 1
Scopulariopsis brevicaulis is known as the most common etiological factor of the mould toenail infections. There are also reports indicating that S. brevicaulis could cause organ and disseminated infections. Nowadays microscopic observations from the direct sample and culture are crucial for the appropriate recognition of the infection. In this paper a PCR-based method for S. brevicaulis detection is presented. The specificity of the reaction was confirmed, as positive results were obtained only for tested S. brevicaulis isolates and no positive results were obtained for other moulds, dermatophytes, yeast-like fungi, and human DNA.
2

First report on echinocandin resistant Polish Candida isolates

100%
Mroczynska M., Brillowska-Dabrowska A.
Acta Biochimica Polonica
|
2019
|
tom 66
|
nr 3
3

Oporność izolatów Candida krusei na kaspofunginę

100%
Mroczynska M., Brillowska-Dabrowska A.
Postępy Mikrobiologii. Suplement
|
2017
|
tom 56
|
nr 2
4

The detection of food components capable of preventing DNA oxidative damage by restriction analysis

81%
Kolodziejski D., Brillowska-Dabrowska A., Bartoszek A.
Polish Journal of Food and Nutrition Sciences
|
2011
|
tom 61
|
nr Suppl.1
5

Real-time PCR approach in dermatophyte detection and Trichophyton rubrum identification

81%
Kobylak N., Bykowska B., Nowicki R., Brillowska-Dabrowska A.
Acta Biochimica Polonica
|
2015
|
tom 62
|
nr 1
6

The use of a one - step PCR method for the identification of Microsporum canis and Trichophyton mentagrophytes infection of pets

81%
Dabrowska I., Dworecka-Kaszak D., Brillowska-Dabrowska A.
Acta Biochimica Polonica
|
2014
|
tom 61
|
nr 2
 Introduction: Dermatophytes are a closely related group of keratinophilic fungi. They encompass important etiological agents of superficial fungal infections. These fungi are able to invade keratinized tissues of humans and animals, causing dermatophytosis (ringworm) of hair, nails or skin. The aim: Traditional diagnostics of ringworm is based on morphological identification of cultured fungi and is time-consuming. Materials and methods: In this study, we applied a method patented by Brillowska-Dabrowska and coworkers (Brillowska-Dąbrowska A, Saunte DM, Arenderup MC, 2007, Five-hour diagnosis of dermatophyte nail infections with specific detection of Trichophyton rubrum. J Clin Microbiol 45: 1200-1204) which involves extraction of fungal DNA and PCR amplification with pan-dermatophyte primers to confirm the presence of dermatophytes. Results: The method used here is able to confirm the presence of dermatophyte DNA in pure cultures in less than 5 hours.
7

PCR and real-time PCR assays to detect fungi of Alternaria alternata species

81%
Kordalewska M., Brillowska-Dabrowska A., Jagielski T., Dworecka-Kaszak B.
Acta Biochimica Polonica
|
2015
|
tom 62
|
nr 4
8

Recombinant thermostable AP exonuclease from Thermoanaerobacter tengcongensis: cloning, expression, purification, properties and PCR application

81%
Dabrowski S., Brillowska-Dabrowska A., Ahring B. K.
Polish Journal of Microbiology
|
2013
|
tom 62
|
nr 2
Apurinic/apyrimidinic (AP) sites in DNA are considered to be highly mutagenic and must be corrected to preserve genetic integrity, especially at high temperatures. The gene encoding a homologue of AP exonuclease was cloned from the thermophilic anaerobic bacterium Thermoanaerobacter tengcongensis and transformed into Escherichia coli. The protein product showed high identity (80%) to human Ape1 nuclease, whereas to E.coli exonuclease III – 78%. This is the first prokaryotic AP nuclease that exhibits such high identity to human Ape1 nuclease. The very high expression level (57% of total soluble proteins) of fully active and soluble His₆ -tagged Tte AP enzyme with His₆ -tag on C-terminal end was obtained in Escherichia coli Rosetta (DE3) pLysS. The active enzyme was purified up to 98% homogeneity in one chromatographic step using metal-affinity chromatography on Ni²⁺-IDA-Sepharose resin. The yield was 90 mg (14 000 kU) of pure His₆ -tagged Tte AP (153 kU/mg) from 1 liter of culture. The optimal conditions of Tte AP endo-, exonuclease and 3’-nuclease activity were investigated using fluorescein labeled dsDNA with inserted AP sites and ssDNA. Optimal Tte AP endonuclease activity was observed at 70–75°C, pH 8.0 and at low Mg²⁺ concentration (0.5 mM). Higher Mg2+ concentration (> 1 mM) enhanced 3’-5’ exonuclease activity and at Mg²⁺ concentration > 2.0 mM 3’ nuclease activity was observed.Because of the endonuclease activity of Tte AP exonuclease, the enzyme was applied in PCR amplification of long DNA templates. Tte AP exonuclease eliminated AP-sites in DNA template and improved the efficiency of DNA amplification.
9

New PCR test for detection on Candida glabrata based on the molecular target chosen by RAPD technique

81%
Olchawa A., Krawczyk B., Brillowska-Dabrowska A.
Polish Journal of Microbiology
|
2013
|
tom 62
|
nr 1
Rapid, reliable diagnosis is a necessary condition for the successful treatment of infections. Such diagnostic assays are continually being developed. The paper presents a method for selecting the molecular target for PCR-based diagnostics based on the comparison of RAPD patterns. A sequence encoding Candida glabrata CBS138 hypothetical protein was selected. The limit of detection for PCR and real-time PCR reactions with DNA extracted from blood samples spiked with Candida glabrata was estimated at 1 CFU/ml. The application of the assays developed in this study would thus seem to be promising as a complementary method in the diagnostics of C. glabrata infections.
10

Examination of cyp51A and cyp51B expression level of the first Polish azole resistant clinical Aspergillus fumigatus isolate

71%
Brillowska-Dabrowska A., Mroczynska M., Nawrot U., Wlodarczyk K., Kurzyk E.
Acta Biochimica Polonica
|
2015
|
tom 62
|
nr 4
11

Toxoplasma gondii - construction of expression systems and production of recombinant antigens for immunodiagnostics

71%
Hiszczynska-Sawicka E., Brillowska-Dabrowska A., Dabrowski S., Myjak P., Kur J.
Cellular and Molecular Biology Letters
|
2001
|
tom 06
|
nr 3A
775
12

Oporność izolatów Aspergillus fumigatus na izawukonazol

71%
Kurzyk E., Adamik M., Nawrot U., Wlodarczyk K., Brillowska-Dabrowska A.
Postępy Mikrobiologii. Suplement
|
2017
|
tom 56
|
nr 2
13

Toxoplasma gondii - konstrukcja systemów ekspresyjnych i produkcja antygenów rekombinowanych do immunodiagnostyki

71%
Hiszczynska-Sawicka E., Brillowska-Dabrowska A., Dabrowski S., Myjak P., Kur J.
Wiadomości Parazytologiczne. Suplement
|
2001
|
tom 47
|
nr 1
14

Zastosowanie antygenów rekombinowanych w diagnostyce toksoplazmozy

61%
Pietkiewicz H., Kur J., Myjak P., Dabrowski S., Hiszczynska-Sawicka E., Brillowska-Dabrowska A., Andrzejewska I.
Wiadomości Parazytologiczne. Suplement
|
2001
|
tom 47
|
nr 1
15

Toxoplasma gondii recombinant antigenes for diagnostic application

61%
Pietkiewicz H., Kur J., Myjak P., Dabrowski S., Brillowska-Dabrowska A., Andrzejewska I.
Cellular and Molecular Biology Letters
|
2001
|
tom 06
|
nr 3A
811
16

Relation of the polymorphism of cyp51A sequence and the susceptibility of Aspergillus fumigatus isolates to triazoles determined by commercial gradient test (Etest) and by reference methods

51%
Nawrot U., Sulik-Tyszka B., Kurzyk E., Mroczynska M., Wlodarczyk K., Wroblewska M., Basak G. W., Brillowska-Dabrowska A.
Acta Biochimica Polonica
|
2017
|
tom 64
|
nr 4
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