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RAPD-PCR of Trichoderma isolates and in vitro antagonism against Fusarium wilt pathogens of Psidium guajava L.

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Gupta V. K., Misra A. K., Gupta A., Pandey B. K., Gaur R. K.

Journal of Plant Protection Research

2010

tom 50

nr 3

The paper concerns randomly amplified polymorphic DNA RAPD-PCR analysis of seven Trichoderma speciesisolates and their in vitro antagonism against wilt pathogens of Psidium guajava L. viz. Fusarium oxysporum f. sp. psidii (F. o.f. sp. psidii) and Fusarium solani. Out of 10 RAPD oligonucleotides (OPA 1-OPA 10) tested, seven markers OPA 1, 3, 5, 7, 8, 9 and10 efficiently differentiated the isolates of Trichoderma and showed reproducible banding patterns. A total of 248 bands were obtained from these markers along with a 61.84% per cent similarity among the seven isolates of Trichoderma.From the seven isolates of Trichoderma, isolates T. harzianum, T.virens and T. viride were evaluated for in vitro efficacy against F. o. f. sp. psidii and F. solani. Per cent inhibition was maximum by direct use of Trichoderma spp. in dual cultures against both pathogens, F. o. f. sp. psidii and F.solani. Two species, T. virens and T. viride were superior in inhibiting the growth of both Fusarium spp. Fusarium isolates showed intra species variability.

Ograniczanie wyników

1 Journal of Plant Protection Research

1 Gaur R. K.

1 Gupta A.

1 Gupta V. K.

1 Misra A. K.

1 Pandey B. K.

1 2010

Wyniki wyszukiwania

RAPD-PCR of Trichoderma isolates and in vitro antagonism against Fusarium wilt pathogens of Psidium guajava L.