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The trematode parasite Fasciola hepatica infects a wide variety of mammals, causing considerable economical losses in domestic animals. It is also an important zoonosis. The major pathology of fasciolosis is caused by immature flukes migrating in the liver parenchyma and later by adult flukes in the bile ducts. Egg related immunopathology, commonly observed in the closely related parasite Schistosoma sp., is not normally expected in fasciolosis because the parasite matures and produces eggs in the bile ducts. However, the paper reports a case of Fasciola hepatica-egg induced granuloma in a bovine liver parenchyma and discusses its significance in the pathogenesis of fasciolosis.
This study was carried out on cattle to detect the seroprevalence of theileriosis and babesiosis around the Antakya province. A total of 214 randomly selected cattle were examined from selected locations for Theileria annulata, Babesia bigemina, B. bovis and B. divergens. Blood samples were collected from the cattle by jugular vene puncture to obtain sera for IFAT. Thin blood smears were prepared from the punctured ear veins of each animal. The blood smears were stained with 5% Giemsa's stain and examined microscopically at 100 × magnification. None of the Babesia species was detected but T. annulata observed in 5 (2.33%) blood smears. The sera were tested for the presence of antibodies to the T. annulata, Babesia bigemina, B. bovis and B. divergens by IFAT. Antibodies were detected against T. annulata in 24 and B. bigemina in 2 sera of the tested 214 cattle. Antibodies for B. bovis and B. divergens were not detected in any sera. It has been concluded that detailed molecular biological, serological and epidemiological studies needed to clarify the genetic and antigenic diversity of the blood parasites in Turkey.
In the present study, we aimed to provide information on the serum content of sialic acids (TSA, LBSA, and PBSA), total antioxidant capacity (TAC), and adenosine deaminase (ADA) activity in cattle affected with naturally acquired theileriosis and anaplasmosis. A total of 55 Holstein cattle, comprising of 15 clinically healthy control animals, 20 cattle with theileriosis, and 20 with anaplasmosis, were used. Diagnosis was based on clinical signs, Giemsa stained blood or lymph node aspirate, and PCR assay. For the PCR assay, Tams 1 primers were used. The obtained results suggested that the concentration of sialic acids and ADA activity were significantly higher; and TAC were significantly lower in the theileriosis and anaplasmosis groups in contrast to the control group. In conclusion, the increased level of sialic acids and ADA in theileriosis and anaplasmosis may be attributable to the stimulation of the host immune response. In contrast, the reduced level of TAC may reflect a decrease in the antioxidant capacity.
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