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Electrochemical impedance spectroscopy was used for the study of two-component lipid membranes. Phosphatidylcholine and ceramide were to be investigated, since they play an important biochemical role in cell membranes. The research on biolipid interaction was focused on quantitative description of processes that take part in a bilayer. Assumed models of interaction between amphiphilic molecules and the equilibria that take place there were described by mathematical equations for the studied system. The possibility of complex formation for two-component system forming bilayers was assumed that could explain the deviation from additivity rule. Equilibria were described by mathematical equations that were further verified experimentally. The determined values of parameters (stability constant, molecular area of complex, capacitance and conductance of the lipid membranes formed from molecules and complexes) were used for calculation of model curves. The comparison of model curves and experimental points verified the assumed model
Capacity and electric resistance of lipid membranes composed of lecithin and cholesterol were determined. The components were chosen for the study because they were present in biological membranes. Capacitance of the lecithin and cholesterol membranes amounts to 0.38 and 0.61 μF/cm2, and resistance to 1.44xl04 and 2.12x 106 Ω cm2, respectively. A 1:1 complex appears as a result of lecithin-cholesterol membrane formation. Parameters of the membrane formed of the lecithin-cholesterol complex were determined: surface concentration (Γ3), capacitance (C3), and conductance (R 31), as well as the stability constant (K) of the complex. The mean values of those magnitudes are as follows: 4.265xl0-6 mol/m2, 0.54 μF/cm2, 1.381xl0-6 Ω-1 cm-2 and 3.748x107, respectively.
We studied the electric properties of phosphatidylcholine bilayers modified with crown ether (dibenzo[18] crown-6). The studies were carried out for various crown ether concentrations in forming solutions and various potassium ion concentrations in electrolyte solutions. The presence of crown ether in the membrane influences the membrane's impedance; there is a reduction in its resistivity, a decrease in its resistance of phase transfer and an increase in its capacity of phase transfer with an increase in crown ether concentration in the bilayer and in K+ ion concentration in the electrolyte solution.
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