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The paper contains results of a faunistic research during the collecting trip by three members of the Silesian Entomological Society in Italy and Croatia on 27 May - 4 June 2000. For all collected species the localities are given, for some most interesting species also short comments are provided.
The aim of the study was the evaluation of the influence of cadmium on protein profile of flax varieties (Linum usitatissimum L.). Linola and Norlin explants were cultured on control medium Dorothy and with addition of 25 and 75 mg/l Cd(NO3)2. Extracts were separated on DEAE-Cellulose (20 mM Tris-HCl buffer, 0.2-1 M NaCl). Protein content was evaluated by measuring the absorbance at wavelengths 280 and 254 nm. Linola was characterized by proteins occurrence in fractions eluted by 0.4 and 0.6 M NaCl at 25 mg/l of Cd(NO3)2, at 75 mg/l and additionally by 0.3 M NaCl. Norlin showed response in the form of proteins appeared at 0.2, 0.4, 0.5 and 0.6 M NaCl gradient at 25 mg/l of cadmium, at higher concentration in fractions eluted by 0.2, 0.5 and 0.6 M NaCl. Electrophoretic analysis showed an increase in the protein bands intensity above 60 kDa and under 52 kDa in extracts from flax cultivated with Cd(NO3)2. Studies showed appearance of new proteins during stress condition.
Overexpression of SGTP and/or MT may contribute to various carcinogenic processes and to resistance to anticancer treatment. The importance of these proteins, although clearly established in solid tumours, has not been fully understood in haematopoietic neoplasm. The aim of this study was to determine the expression of MT and SGTP in the bone marrow of patients with MPD. Twenty paraffin-embedded bone marrow core biopsy specimens from newly diagnosed patients with MPD were evaluated — osteomyelofibrosis (OMF), n = 9 and chronic myelocytic leukaemia (CML), n = 11. We demonstrate increased SGTP and MT expression in the bone marrow of MPD patients. In our study levels of MT in OMF patients were higher than in CML. This suggests that MT expression may correlate with bone marrow fibrosis. These data, although based on a relatively small number of patients, raise the possibility that SGTP and MT may play a role in the pathogenesis of MPD. The clinical significance of this phenomenon needs further investigation.
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