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1

Molecular cloning and characterisation of the genes coding for passenger domain of the PmpD from Chlamydophila abortus CP16

100%
Yang J., Ling Y., Li S., He C.
Bulletin of the Veterinary Institute in Puławy
|
2009
|
tom 53
|
nr 4
The passenger domain gene of PmpD of swine Chlamydophila abortus CP 16 was amplified, subcloned into the pet-32a(+) vector, then sequenced, and expressed in Escherichia coli BL21(DE3). The target protein was identified by Western blotting. It was demonstrated by sequence analysis that the passenger domain gene of Pomp18 contained 1317 bp that encoded a protein of 439 amino acids. The homology of the target gene was found to be 97.0%, 97.0%, 97.2% , 52.6%, 44.6%, and 87.5%, respectively, identical to that of the CPD13, S26/3, 1B, TW-183, TW-396, and CAL-10 strains of C. abortus. The recombinant 65.3 kDa protein was identified by both SDS-PAGE and Western blotting. A positive reaction was observed with swine positive serum, while neither sheep serum nor avian serum reacted with the target protein. In conclusion, the passenger domain of PmpD gene was at first cloned and expressed successfully. The recombinant protein showed the species reaction and might play a major role in C. abortus infection.
2

Protective effect of pCI-neo vector-mediated DNA vaccine against Chlamydophila abortus in mice

76%
Li S., He C., Ling Y., Wang H., Zhao J., Yao B.
Bulletin of the Veterinary Institute in Puławy
|
2008
|
tom 52
|
nr 2
In the presented study, omp-1 gene coding main outer membrane protein of Chlamydophila abortus was cloned into pCI-neo and pcDNA3.1 as delivery vehicles for DNA vaccination. Thirty-six BALB/c mice were randomly assigned to three groups and inoculated intramuscularly with: 1) 100 µg of pCI-neo, 2) 100 µg of pCI-neo::MOMP, and 3) 100 µg of pcDNA3.1::MOMP. All animals were vaccinated three times at 14 d intervals. The results showed that mice given pCI-neo::MOMP developed a higher IgG antibody level, high T lymphocytes proliferations, and high titres of IFN-γ and IL-2, than mice given pcDNA3.1::MOMP, which induced moderate antibody levels, less T lymphocyte proliferations and lower cytokine levels. No significant difference of TNF-α was observed in above groups. Additionally, IgG2a and Ig2b were the predominant isotypes on day 44, suggesting a high level of Th1 stimulation. Mice given the pCI-neo::MOMP also elicited a higher chlamydial clearance and a better protection than mice with pcDNA3.1::MOMP did. Immunisation with pCI-neo::MOMP vaccine may provide novel ways for active immunisation strategy against Chlamydophila abortus.
3

Assessing the microbial communities in four different daqus by using PCR-DGGE, PLFA, and biolog analyses

64%
Ling Y., Li W., Tong T., Li Z., Li Q., Bai Z., Wang G., Chen J., Wang Y.
Polish Journal of Microbiology
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2020
|
tom 69
|
nr 1
4

Vital staining of bacteria by sunset yellow pigment

64%
Gao P., Sun C., Li Y., Zou X., Wu X., Ling Y., Luan C., Chen H.
Polish Journal of Microbiology
|
2017
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tom 66
|
nr 1
In this study, we describe a method for discriminating pathogenic bacteria with a dye. First, we determined that among several colours tested, the sunset yellow pigment easily coloured Escherichia coli bacteria yellow. Next, we demonstrated that E. coli O157:H7, Shigella flexneri O301, Staphylococcus aureus and Bacillus subtilis could all be well marked by sunset yellow pigment. Finally, we performed bacterial viability assays and found there was no effect on bacterial growth when in co-culture with sunset yellow. Our results suggest that sunset yellow is suitable pigment to dye microorganisms.
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