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1

Badania nad wlasciwosciami fizyczno-chemicznymi dehydrogenazy 15-hydroksyprostaglandynowej z lozyska krow

100%
Kankofer M., Wiercinski J., Kedzierski W.
Annales Universitatis Mariae Curie-Skłodowska. Sectio DD:Medicina Veterinaria
|
1995
|
tom 50
225-234
2

Erythrocyte antioxidative enzymes in experimentally induced osteopenia in rats

100%
Radzki R. P., Bienko M., Albera E., Kankofer M.
Bulletin of the Veterinary Institute in Puławy
|
2012
|
tom 56
|
nr 4
The study aimed at the determination of dynamic relationship between mineralisation processes and antioxidative/oxidative status during the development of osteopenia. One hundred and two healthy female Wistar rats at the age of 2 months and initial body weight of 200 g were used in the experiment. The rats were divided into control (CON, n=6), sham operated (SHO, n=48), and ovariectomised (OVX, n=48) groups. Animals from SHO (n=6) and OVX (n=6) groups were sacrificed every week during 8 weeks of the experiment in order to detect dynamic changes in examined parameters. The samples were collected weekly from day 7 to day 56. The femora were examined with the use of DXA (bone mineral density) and pQCT (area, mineral content, volumetric density of trabecular and cortical part of distal femora). The pQCT scans were performed 5 mm from distal end of the tibia. Hie determination of activity of glutathione peroxidase (GSH-Px) and superoxide dismutase (SOD) in haemolysates of erythrocytes were performed spectrophotometrically. Obtained data showed wave like changes in both enzyme activities and bone parameters and indicated the importance of the 2nd -3rd and 5th -6th week after surgery as a key moment for bone metabolism and activity of enzymatic antioxidative defence during the development of osteopenia induced by bilateral ovariectomy. The obtained results proved that alterations in activity of GSH-Px and SOD, and pQCT ahead the changes registered by DXA by 7 d.
3

Lipid peroxidation as the additional indicator of transport stress in broilers

100%
Tokarzewski S., Wernicki A., Kankofer M., Urban-Chmiel R.
Polish Journal of Veterinary Sciences
|
2004
|
tom 07
|
nr 2
4

Antioxidative-related activities of lactoferrin and lactoperoxidase in milk from cows with different forms of mastitis

100%
Szczubial M., Kankofer M., Dabrowski R.
Bulletin of the Veterinary Institute in Puławy
|
2011
|
tom 55
|
nr 1
Milk samples were taken from cows with acute, subacute, chronic, and subclinical mastitis and from healthy cows. The mean activity of lactoferrin (LF) in milk from mastitic cows ranged from 8.9 ±3.0 to 12.1 ±6.9 mU/g protein and was significantly lower than that in milk from healthy cows (29.5 ±15.0 mU/g protein). In group of mastitic cows the highest LF activity was found in cows with chronic mastitis, and the lowest in those with subclinical mastitis. The lactoperoxidase activity in cows with clinical and subclinical mastitis was significantly higher in comparison with healthy cows (1.3 ±1.1 mU/g protein) ranging from 5.5 ±2.6 mU/g protein in subclinical mastitic cows to 8.4 ±5.0 mU/g protein in chronic mastitic cows. Lower LF activities in cows with mastitis than in healthy animals may lead to a decreased antioxidant defence system in mastitic cows.
5

The dynamics of changes in erythrocyte glutathione peroxidase activity and serum selenium content during the periparturient period in sows

100%
Szczubial M., Kankofer M., Wawron W., Krasucki J.
Polish Journal of Veterinary Sciences
|
2004
|
tom 07
|
nr 1
The aim of the present study was the description of the dynamics in erythrocyte GSH-Px activity and serum selenium content changes in sows during 14 days before and 14 days after parturition. The experiment was performed on 36 gilts of 1-3 years old. All animals came from the same farm with a closed production system and were fed the same diet of unknown selenium content. Blood was collected 8 times: 13-14 days before parturition, 6-7 days before parturition, 48-72 hours before parturition, and 12-24 hours before parturition, as well as 12-24 hours after parturition, 48-72 hours after parturition, 6-7 days after parturition and 13-14 days after parturition, always after feeding. Blood was centrifuged to obtain serum and erythrocytes were hemolysed. Erythrocyte GSH-Px activity was determined by the Paglia and Valentine method and expressed in U per g Hb. Serum selenium content was determined by the atomic absorption method. GSH-Px activity 14 days before parturition reached 13.38 U/g Hb and significantly decreased around the 7th day before parturition to 11.26 U/g Hb. 72 hours before parturition the activity of the enzyme increased to 16.85 U/g Hb. Between the 72nd hour before and the 14lh day after parturition GSH-Px activity remained constant. Serum selenium content did not differ significantly in the examined gilts and ranged between 88.9 ug/L and 121.87 ug/L. 24 hours before and 24 hours after parturition the selenium content reached 96.55 ug/L and 88.91 ug/L respectively.
6
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Content available

Assessment of lipid and protein peroxidation markers in non-pregnant and pregnant female dogs

88%
Szczubial M., Kankofer M., Dabrowski R., Bochniarz M., Urban-Chmiel R.
Polish Journal of Veterinary Sciences
|
2015
|
tom 18
|
nr 1
The aim of the study was to investigate oxidative stress during normal pregnancy in female dogs based on an evaluation of plasma markers for lipid and protein peroxidation. Twenty clinically healthy female dogs (10 non-pregnant and 10 pregnant) were used in the study. Blood samples from the pregnant animals were collected at 19-21, 38-40, and 56-58 days of pregnancy. Blood samples from non-pregnant female dogs were obtained between 20 and 35 days after ineffective breeding. As indicators of oxidative stress, we measured the following using spectrophotometric and spectrof-luorimetric methods: thiobarbituric acid reactive substances (TBARS), radical cations of N,N, die-thylpara-phenylene diamine (RC-DEPPD), sulfhydryl groups (SH groups), bityrosine and formyl-kynurenine. The mean plasma TBARS concentration in the pregnant dogs (0.486 ± 0.071 - 0.581 ± 0.191 |imol/g protein) was significantly higher (p<0.05) than that found in the non-pregnant animals (0.274 ± 0.111 |imol/g protein). A marked, although not significant, decrease in SH group content, as well as an increase in bityrosine and formylkynurenine concentration were concurrently observed in the pregnant dogs. No significant differences were found in terms of the studied markers in the pregnant animals when comparing the values obtained during the investigated periods of pregnancy, although there was a progressive decrease in TBARS concentration and a progressive increase in RC-DEPPD, bityrosine and formylkynurenine contents. Our findings suggest that normal pregnancy in female dogs is associated with oxidative stress. Further studies are necessary to establish the physiological ranges of antioxidative/oxidative profiles in pregnant dogs and to explain if and how the intensity of oxidative stress might contribute to the risk of the complications of pregnancy.
7

Transport jako czynnik wzmagajacy reakcje stresowe u brojlerow kurzych

88%
Tokarzewski S., Wernicki A., Kankofer M., Urban-Chmiel R., Arciszewski M.
Annales Universitatis Mariae Curie-Skłodowska. Sectio DD:Medicina Veterinaria
|
2006
|
tom 61
127-134
8
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Concentration of serum amyloid A and ceruloplasmin activity in milk from cows with subclinical mastitis caused by different pathogens

88%
Szczubial M., Dabrowski R., Kankofer M., Bochniarz M., Komar M.
Polish Journal of Veterinary Sciences
|
2012
|
tom 15
|
nr 2
The aim of the present study was to determine the concentration of serum amyloid A (SAA) and the activity of ceruloplasmin (Cp) in milk from cows with subclinical mastitis caused by different pathogens. Eighty-four milk samples from cows with subclinical mastitis and fourteen milk samples from healthy cows were examined. SAA concentration was determined using the commercial ELISA kit (Tridelta Development Ltd., Greystones, Wicklow, Ireland). Cp activity was assessed spectrophotometrically, using the Rice method. The results reveal that the concentration of SAA (with exception of CNS) and activity of Cp in cow milk can be regarded as markers of subclinical mastitis, irrespective of the microorganism inducing the disease. In conclusion, measurement of SAA and Cp in milk samples could be a useful method in diagnosing subclinical mastitis in cows, but the method should be adapted for field use.
9

Wplyw stresu transportowego na wybrane wskazniki odpornosci komorkowej u bydla

88%
Wernicki A., Urban-Chmiel R., Mikucki P., Puchalski A., Kankofer M.
Medycyna Weterynaryjna
|
2003
|
tom 59
|
nr 07
608-611
10

Oxidative-antioxidative status of blood plasma in bitches with mammary gland tumours

88%
Szczubial M., Kankofer M., Albera E., Lopuszynski W., Dabrowski R.
Bulletin of the Veterinary Institute in Puławy
|
2008
|
tom 52
|
nr 2
The aim of the study was the estimation of oxidative/antioxidative status of bitches with mammary gland tumours by the determination of lipid peroxidation intensity, concentration of glutathione and β-carotene as well as total antioxidant capacity (TAC) in blood plasma. The experiment was carried out on 18 bitches with spontaneously occurring mammary gland tumours (12 with malignant tumours and 6 with benign tumours) and 6 clinically healthy controls. The intensity of lipid peroxidation did not differ significantly among the examined groups of animals. The concentration of glutathione was higher in both neoplasm groups than in healthy bitches, but the differences were not significant. The concentration of β-carotene in plasma was similar in cases of malignant and benign tumours, but was significantly lower (P<0.05) than in healthy controls. TAC of plasma was lower in bitches with tumours than in healthy animals. Significant difference was noticed between malignant tumours and controls (P<0.01). In conclusion, the alterations in antioxidative status occur in bitches with mammary gland tumours, suggests the presence of general antioxidative stress. A necessity of more frequent sample collection for the detection of dynamic changes in peroxidation process revealing the intensity of oxidative stress in bitches suffering from mammary gland tumours should be considered.
11

Concentration of serum amyloid A and activity of ceruloplasmin in milk from cows with clinical and subclinical mastitis

88%
Szczubial M., Dabrowski R., Kankofer M., Bochniarz M., Albera E.
Bulletin of the Veterinary Institute in Puławy
|
2008
|
tom 52
|
nr 3
The aim of the study was to determine the concentration of serum amyloid A (SAA) and the activity of ceruloplasmin (Cp) in milk from cows with clinical mastitis of various severities and with subclinical mastitis in aspect of their usefulness for the detection of mastitis in cows. The concentration of SAA was determined using the commercial ELISA kit. The activity of Cp was determined according to the Rice et al., method. The mean concentration of SAA in milk from cows with mastitis ranged from 4.47 to 322.26 µg/mL. The mean SAA concentration in milk from healthy cows was 11.67 (±7.40) µg/mL and was significantly lower (P<0.01) compared to that in milk from cows with the particular forms of mastitis. The activity of Cp in milk from cows with mastitis ranged from 3.00 to 18.83 U/g of protein. Both in clinical and subclinical mastitis the activity of Cp was significantly higher (P<0.001) compared to that of milk from cows with healthy mammary glands (1.20 ±0.42 U/g of protein). The findings revealed that both the SAA concentration and Cp activity were sensitive indicators of inflammatory processes in the udder, even those graded as mild. Their determination in milk may be a reliable and non-invasive diagnostic method to detect mastitis, particularly its subclinical form.
12

The influence of transport stress on the humoral immunological response of calves induced by Mannheimia haemolytica leukotoxin

88%
Wernicki A., Urban-Chmiel R., Mikucki P., Puchalski A., Kankofer M.
Polish Journal of Veterinary Sciences
|
2003
|
tom 06
|
nr 1
In the contemporary systems of cattle production, transport stress is the most essential polyetiological factor responsible for inducing unfavourable reactions in the animals. The main reason for this phenomenon is the immunosuppressive effect of steroid hormones on cellular and humoral protective mechanisms. The purpose of the present study was to establish the relationship between the Cortisol concentration as an indicator of the stress reaction occuring directly after the transportation of calves and the specific humoral immune response to the leukotoxin (Lkt) antigen produced by the M. haemolytica strain. The experiment was carried out on 19 clinically healthy calves, weighing about 100 kg and transported by track for about 2 hours. After the delivery of the animals for feeding to the traditional cattle-house, the calves were immunized s.c.: group I with 1 ml Lkt (in conc. - 10 μg/ml) with 1 ml of adjuvant on the 1st and 14th day after the transportation, group II with the same Lkt doses on the 3rd and 16th day after the transportation. The animals of the control group were vaccinated on the 1st and 14th day after the transportation with the twice diluted adjuvant. In examined sera the Cortisol concentrations and the level of Lkt antibodies were measured by ELISA test. The cytotoxin neutralizing (CN) antibody level (cytotoxity assay) was determined with a simple visual assay. The study revealed, significant differences (P ≤ 0.01) in serum Cortisol leves between the control and experimental animals. The analysis of the absorbance of the sera in both groups immunized with Lkt showed substantial differences (P ≤ 0.05) from the 6th through to 22nd day of the experiment compared with the control group. The analysis of the results of CN antibody titers showed no differences between the sera from group I and II. Based on the results obtained in this experiment it can be assumed, that a short transportation stress has no important influence on the level of specific humoral anti-Lkt response.
13

Badania nad wpływem czynników środowiskowych na częstotliwość wczesnych upadków cieląt

76%
Glazer T., Zebracki A., Kuleta Z., Rotkiewicz T., Zdunczyk S., Kankofer M.
Acta Academiae Agriculturae ac Technicae Olstenensis. Veterinaria
|
1990
|
tom 19
14

Changing morphology in the human cotyledon subjected to dual perfusion in vitro exposed to variable magnetic fields

64%
Lopucki M., Lancut M., Kankofer M., Rogowska W., Czerny K., Jedrych B., Pietruszewski S., Stachowicz N., Kornarzynski K., Kotarski J.
Polish Journal of Environmental Studies
|
2004
|
tom 13
|
nr Suppl.2, Part 1
The paper presents the results of morphological examinations of the human cotyledons perfused in vitro and exposed to variable magnetic field (MF) during the 180-minute experiment. The cotyledon biopsies were collected immediately after perfusion and morphologically examined using the electron microscope. The control group C (10 perfusions) was not exposed to MF. In the experimental group E, (10 perfusions), the cotyledons were exposed to the 2mT, 50Hz variable magnetic field while in the experimental group E2 (10 perfusions) the 5mT, 50 Hz was used. In the groups E, and E2, numerous indentations of the sheath (areola) in the syntrophoblast nuclei were found, condensed (thickened) nuclear chromatin right beneath the sheath was substantially reduced and pyknosis was observed in some nuclei. The villi revealed widened vascular - epithelial membrane resulting from the oedema of the endothelial cells. Moreover, an increased number of collagen fibres in the villi and decreased number of active mitochondria were observed in the group E2
15

The influence of the magnetic field on the glucose consumption and lactic acid production in the in vitro perfused human placental cotyledon

64%
Lopucki M., Kankofer M., Pietruszewski S., Kornarzynski K., Rogowska W., Stachowicz N., Semczuk-Sikora A., Czekierdowski A., Kotarski J.
Polish Journal of Environmental Studies
|
2004
|
tom 13
|
nr Suppl.2, Part 1
Objective: The biological effects of variable magnetic fields (MF) generated by widely used electrical devices on living organisms are not well understood. However MF may be potentially hazardous for pregnant women, this problem was not profoundly studied. The aim of this study was to evaluate the influence of the variable magnetic field (MF) on the placental metabolism by measurement of glucose consumption and lactate production in the human placenta under dual recirculating perfusion conditions. Material and methods: Altogether 20 term human placental cotyledons were exposed to homogeneous variable MF of B = 2 mT and f = 50 Hz (n=10; group E,) or of B = 5 mT and f = 50 Hz (n=10; group E2) during 3 hours dual close perfusion in vitro. 10 term placental cotyledons were perfused without an exposure to the MF and they served as a control group. Results: The results showed no effect of used MF on the glucose consumption either in group E, or in group E2in. There was also no influence of MF on lactic acid production in group Er In group E2, however, a significant increase of lactic acid production in the fetal circulation from 90lh to 180th minute and in the maternal circulation at 180 minute was noted. Conclusions: An increase in the lactic acid production in the group E2in observed during the experiment may result from hindered oxygen supply to the placenta and intensified anaerobic metabolism of glucose.
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