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The samples of 300 faeces derived from the dogs suspected of canine parvovirosis were tested by the haemagglutination and haemagglutination-inhibition tests according to Carmichael. Out of the samples which had a titre of 4096 ten samples were taken at random to propagate CPV-2 in a continuous FC line of cells. After 72 hours the inoculated cells were examined by the direct immunofluorescent test using a labelled antibody against feline panleukopenia virus. A specific immunofluorescence was detected in cells inoculated with these samples. The specificity of the test was confirmed by blocking of the IF test by means of antibodies against CPV-2 virus.
The purpose of the work was to assess the efficacy of the combined administration of Vetaraxoid and Sofan in the treatment of atopic inflammation of external auditory ducts in dogs. The treatment of 30 dogs with the signs of acute erythema, proliferative inflammation and papillary changes of external auditory ducts was carried out by means of Vetaraxoid (Pfizer) anti-allergic and anti-inflammatory drug and Sofan (Pfizer), an area specific one. A high efficacy of the combined treatment was confirmed. Within 3 months since finishing the treatment no recurrence was noted and a free passage of auditory ducts with proliferative and papillary changes was maintained.
Clinical signs, haematology (leucopenia, lymphopenia, neutropenia) and results at necropsy may only suggest a disease suspected of a parvovirus etiology. Real proof of this kind infection is based on identification of canine parvovirus in faeces of a sick dog. Because of the common occurence of CPV-2 infections in dogs there is need to introduce a rapid test which would make it possible to perform a proper diagnosis immediately after the collection of the samples of faeces in hospital. In this paper the presence of CPV-2 was demonstrated by means of the test developed by On-Site Biotech (Uppsala, Sweden) on 41 of 50 stool samples with clinical signs suggesting parvovirus disease. Of 41 infected dogs 40 were leucopenic (leucocytes ≤ 4.5 G/L), lymphopenic (lymphocytes ≤ 1.3 G/L) and neutropenic (neutrophiles ≤ 4.0 G/L). Of nine dogs uninfected with CPV-2, none showed leucopenia, lymphopenia or neutropenia.
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