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1

Antibacterial activity of Withania somnifera (L.) Dun. organs cultivated in vitro

100%
Furmanowa M., Gajdzis -Kuls D., Starosciak B., Stefanska J.
Herba Polonica
|
1998
|
tom 44
|
nr 4
265-269
Withania somnifera (L.) Dun. (Solanaceae) jest rośliną leczniczą, w stanie naturalnym występującą w regionie subtropikalnym. Głównymi związkami aktywnymi biologicznie, izolowanymi z tego gatunku, są witaferyna A, witanolid D oraz sitoindozydy. Posiadają one właściwości przeciwbakteryjne, przeciwgrzybowe, przeciwnowotworowe, cytotoksyczne, uczulające na promieniowanie, przeciwutleniające, przeciwstresowe, adaptogenne i immunosupresyjne. W pracy przedstawiono efekt działania różnych szczepów Agrobacterium rhizogenes na tworzenie się korzeni transformowanych Withania somnifera. Do wstępnej identyfikacji witaferyny A i witanolidu D, w materiale biologicznym wyhodowanym in vitro, wykorzystano metodę TLC. Te same wartości Rf wskazują, że ekstrakty z korzeni zawierają witanolid D a ekstrakty z zielonych pędów witanolid D i witaferynę A. Metanolowy wyciąg z korzeni transformowanych wykazywał aktywność przeciwbakteryjną w stosunku do Pseudomonas aeruginosa, Escherichia coli, Micrococcus flavus. Podobny ekstrakt z pędów zielonych działał na bakterie Staphylococcus aureus i Micrococcus flavus; nie wykazywał aktywności w stosunku do Enterobacteriaceae.
2

Mikrorozmnazanie Withania somnifera [L.] Dun. roznymi metodami

100%
Furmanowa M., Gajdzis-Kuls D., Starosciak B., Ruszkowska J.
Zeszyty Naukowe Akademii Rolniczej w Krakowie. Sesja Naukowa
|
1997
|
tom 50
577-579
3

Opornosc szczepow Staphylococcus aureus na czwartorzedowe sole amoniowe i chlorheksydyne

88%
Stefanska J., Mlynarczyk G., Mlynarczyk A., Starosciak B., Luczak M.
Medycyna Doświadczalna i Mikrobiologia
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2002
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tom 54
|
nr 3
191-197
Badano poziom wrażliwości 90 szczepów Staphylococcus aureus izolowanych od pacjentów hospitalizowanych oraz pochodzących ze środowiska pozaszpitalnego na wybrane czwartorzędowe sole amoniowe i chlorheksydynę.
4

Wykrywanie genow smr i qacA u szczepow Staphylococcus aureus z zastosowaniem metody PCR

88%
Stefanska J., Mlynarczyk G., Mlynarczyk A., Starosciak B., Luczak M.
Medycyna Doświadczalna i Mikrobiologia
|
2002
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tom 54
|
nr 3
183-189
U 31 szczepów Staphylococcus aureus, badano obecność genów smr i qacA przy zastosowaniu metody PCR. Badaniami objęto 22 szczepy MRSA (w tym 20 opornych na czwartorzędowe sole amoniowe) oraz 9 szczepów MSSA.
5
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Assessment of in vitro dynamics of pathogenic Acanthamoeba strains originating from contact lens wearers with infectious keratitis

76%
Padzik M., Starosciak B., Szaflik J. P., Pietruczuk- Padzik A., Siczek P., Chomicz L.
Annals of Parasitology
|
2016
|
tom 62
|
nr 4
Recently, incidents of Acanthamoeba keratitis, the vision-threatening eye disease, are reported with increasing frequency worldwide, particularly in contact lens wearers. In our study, the retrospective assessment of in vitro dynamics of subsequent pathogenic Acanthamoeba isolates cultured at 24°C, detected in Polish contact lens wearers with keratitis is presented and results compared with those of environmental A. castellanii Neff strain. There were delayed the proper diagnosis that influenced prolonged and severe course of this eye disease and treatment difficulties. The corneal material was examined directly to visualize developmental amoeba stages for diagnose verification, microbiologically tested for the specific identification of bacteriae and fungi, and in vitro grown in culture medium in temperature 24°C. Among twenty-six keratitis incidents analyzed, eleven were cases of Acanthamoeba keratitis; in the six of them, Acanthamoeba strains and concomitant bacterial and/or fungal infectious agents were detected. In vitro assays showed variability in population density of several clinical strains in the exponential growth phase expressed in various range of overall amoeba number and different proportion between trophozoites and cysts. The clear influence of temperature on the in vitro cultivation of the amoebae was observed: statistically significant lower population dynamics was revealed by most of pathogenic clinical isolates in comparison with those showed by environmental strain. The in vitro monitoring of dynamics of Acanthamoeba strains isolated from infected eyes may be helpful for diagnostics verification, especially in mixed infectious keratitis.
6
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Evaluation of selected oral cavity microbiota – risk factors of management complications in patients with masticatory system disorders

76%
Zawadzki P. J., Perkowski K., Starosciak B., Dybicz M., Baltaza W., Pionkowski K., Chomicz L.
Annals of Parasitology
|
2016
|
tom 62
|
nr 1
The retrospective analysis of data on oral cavity clinical status in relation to microbiota species composition is presented. The research regards patients of different age, with and without congenital malformation, pretreatment assessed for occurrence of pathological changes in the masticatory system. Samples of the swabs collected from each patient (from dental plaque, periodontium and dental pockets) were used for identification of oral protozoans in wet slides and stained preparations; additionally, transmission electron microscope examination was performed. The material was used for in vitro cultures to identify bacteria strains. Clinically, intensity of tissue deteriorations was higher in patients with a congenital disease. Alive Trichomonas tenax and Entamoeba gingivalis, species with confirmed pathogenic impact on oral cavity and neighboring structures, were detected with higher prevalence in older patients. Enterococci, Staphylococcus aureus, various Enterobacteriaceae were more frequently detected in patients with somatic and mental retardations; in mouths of those patients, Klebsiella pneumonia and Pseudomonas aeruginosa opportunistic strains occurred. Masticatory system abnormalities favor colonization of oral cavity by exogenous species and dissemination of infections, especially dangerous for patients with congenital diseases. Oral microbiota assessment and preventive measures may be helpful to avoid subsequent peri-surgery complications.
7
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Wpływ chlorheksydyny na przeżywalność trofozoitów i cyst Acanthamoeba castellanii

76%
Zebrowska J., Chomicz L., Piekarczyk J., Zawadzki P., Piekarczyk B., Starosciak B., Myjak P.
Wiadomości Parazytologiczne. Suplement
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2004
|
tom 50
8
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Comparative assessment of selected intraoral microorganisms – potential factors for peri-surgical management complications

76%
Zawadzki P. J., Starosciak B., Perkowski K., Baltaza W., Padzik M., Pionkowski K., Chomicz L.
Annals of Parasitology
|
2016
|
tom 62
|
nr 3
In this research, a comparative analysis of results of investigations involving different human populations, in terms of a relation between the oral cavity health and the species composition of mouth microbiota is reported. The purpose of this analysis was to identify and assess microorganisms that could cause health complications in patients with neoplasm requiring dental problem-related surgical management. The patients with the oral cancer surgically treated and those without neoplasm were assessed for their oral health: status of teeth, gingiva, periodontium, and occurrence of inflammatory processes. From each patient, microorganisms isolated of periodontium, dental plaque, and dental pocket swabs were identified in wet and stained microscopic preparations; standard microbiology in vitro techniques were also applied to determine the fungal and bacterial strains. The comparative analysis of results of direct microscopic examinations and in vitro cultures assessment indicated significant differences in prevalences of fungi, parasitic oral protozoans and bacteriae in particular patient’s groups. Yeast-like fungi belonging to Candida genus, mostly of C. albicans group, were identified in 93.75% patients with the oral cancer, while in 25% of individuals assessed without neoplasm. E. gingivalis amoebae were only found in 12.5% patients with the serious disease; no trichomonads were detected in all patients analyzed. Among bacteria species, potentially pathogenic Enterobacteriaceae were found in the patients with oral cancer. The pronounced shift in the microbiota species composition in the patients who needed prolonged treatment due to oral cavity cancer, compared to other generally healthy persons has been showed in this analysis.
9

Składniki mikrobiomu jamy ustnej jako czynniki ryzyka zakażeń lokalnych i uogólnionych u pacjentów bez oraz z wadami wrodzonymi narządu żucia

76%
Perkowski K., Zawadzki P. J., Starosciak B., Dybicz M., Padzik M., Marczynska-Stolarek M., Chomicz L.
Postępy Mikrobiologii
|
2016
|
tom 55
|
nr 1
10
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Wystepowanie subklinicznych zakazen jamy ustnej u pacjentow z cukrzyca insulinozalezna

76%
Chomicz L., Szubinska D., Piekarczyk J., Wojtowicz A., Piekarczyk B., Starosciak B., Fiedor P.
Wiadomości Parazytologiczne
|
2004
|
tom 50
|
nr 2
177-180
11

In vitro studies on susceptibility of Acanthamoeba castellanii to selected chemical agents

76%
Chomicz L., Zebrowska J., Piekarczyk J., Starosciak B., Myjak P., Walski M., Kazimierczuk Z.
Acta Parasitologica
|
2005
|
tom 50
|
nr 1
Amoebae of the Acanthamoeba castellanii Neff strain, cultured in bacteria-free condition at room temperature, were tested in vitro for their susceptibility to selected chemical compounds. The amoebae, grown during 4 and 10 days following regular subculturing, were exposed for 24 h to three compounds: the newly synthesized imidazole derivatives (1) 2-methyl-3N-(4-nitrobenzyl)-5-nitro-1H-imidazole designated as AG16; (2) 2-methyl-3N-(3,5-dinitrobenzyl)-5-nitro-1H-imidazole designated as AG17 and to the (3) cationic antiseptic agent chlorhexidine digluconate (CHX). Two different concentrations of the above-mentioned substances were applied. The results showed that all chemicals tested had some amoebicidal effect on 4-day grown population of Acanthamoeba; variations in susceptibility of the amoebae, depending on the kind and concentration of the compounds occurred. It was also observed that several agents changed the relationship between A. castellanii trophozoites and cysts. Chlorhexidine digluconate caused a decrease in percentage of the trophozoites to 88.4% at higher concentration of the compound, 10 (μg/ml (in comparison to 97.8% from the control samples), however, significant increase of the cyst percentage, to 11.6% in assays with this concentration of Chlorhexidine were revealed (2.2% in the control samples). Tendency toward induction of encystment was also apparent when AG17 was applied. Because activation of the dormant cysts can lead to repeated development of amoebae, very important is cysticidal efficacy of tested agents. Among all compounds examined by us, the newly synthesized imidazole derivative AG16 was the most effective. It was expressed as decrease in average number of amoebae (to 655.6 × 10³/ml at concentration of the agent 8 μg/ml; 682.5 × 10³/ml in assays with CHX at concentration 4 μg/ml; 893 × 10³/ml in the control samples) connected with a clear cysticidal effect (1.4% of cysts at concentration 4 μg/ml of AG16).
12

Synthesis, antiprotozoal and antibacterial activity of nitro- and halogeno-substituted benzimidazole derivatives

76%
Kazimierczuk Z., Upcroft J. A., Upcroft P., Gorska A., Starosciak B., Laudy A.
Acta Biochimica Polonica
|
2002
|
tom 49
|
nr 1
Two series of benzimidazole derivatives were sythesised. The first one was based on 5,6-dinitrobenzimidazole, the second one comprises 2-thioalkyl- and thioaryl-sub- stituted modified benzimidazoles. Antibacterial and antiprotozoal activity of the newly obtained compounds was studied. Some thioalkyl derivatives showed remark­able activity against nosocomial strains of Stenotrophomonas malthophilia, and an ac­tivity comparable to that of metronidazole against Gram-positive and Gram-negative bacteria. Of the tested compounds, 5,6-dichloro-2-(4-nitrobenzylthio)-benzimidazole showed the most distinct antiprotozoal activity.
13
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Host-protozoans-bacteria-fungi interrelations in the mouths of patients with systemic illnesses

64%
Chomicz L., Piekarczyk J., Starosciak B., Fiedor P., Piekarczyk B., Wojtowicz A., Szubinska D., Swiderski Z., Rebandel H.
Wiadomości Parazytologiczne
|
2001
|
tom 47
|
nr 4
The aim of this work was to study clinical changes and microorganisms in mouths of three patient groups: with congenital disorders (Cd), dialyzed (D) and control (C). Fifty five patients, 20 to 62 years old were assessed. Swabs and samples of periodontal tissue were used for microscopical study to detect of protozoans and for bacterial and fungal cultures. E. Gingivalis and T. tenax were found in all groups. Three out of Cd patients were infected with Acanthamoeba sp. Fecal bacteria were more often found in D than Cd. In all 30-40 years old D patients, fecal bacteria and various strains of C. albicans occurred. We found that systemie diseases favour instabilities in mouths changing interrelations between protozoa, bacteria and fungi. It may increase risk of clinical complications.
14

Comparative studies on the occurrence of protozoans, bacteria and fungi in the oral cavity of patients with systemic disorders

64%
Chomicz L., Piekarczyk J., Starosciak B., Fiedor P., Piekarczyk B., Szubinska D., Zawadzki P. J., Walski M.
Acta Parasitologica
|
2002
|
tom 47
|
nr 2
The aim of this study was to compare the oral cavity status with the species composition of microorganisms colonizing the mouth of patients with or without systemic diseases. Seventy-seven men and women, 19 to 65-years-old, of four groups: hemodialyzed patients (HD), kidney allograft recipients (Tx), congenitally disordered (Cd) and control (C) patients (without systemic diseases) were examined clinically for the oral cavity status and the microorganisms occurring in the mouth were identified. Swabs and samples taken from dental plaques, pockets and periodontium were used for light and electron microscopical studies as well as for protozoan, bacterial and fungal cultures. Entamoeba gingivalis and Trichomonas tenax were found in the HD, Tx, Cd and C groups (top prevalence 14, 14, 87.5 and 25%, respectively); free-living amoebae (with some features of Acanthamoeba sp.) also occurred in several cases. Staphylococcus aureus bacteria were found in 66% of Cd patients infected with the protozoans. Numerous fecal bacteria and/or fungi Candida albicans have been observed in 41-51-year-old Cd and HD patients. In all patient groups, it was the 41-51-year-olds who showed the highest prevalence of protozoans, bacteria and fungi. The results indicate that metabolic disabilities favour pathological changes in periodontal tissues and may influence the species composition of mixed protozoan, bacterial and/or fungal infections in various ways, in patients with different systemic diseases.
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