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The aim of the study was to determine the effect of pelleted cereal-based feed used in the diet for antelope sitatunga ( ) on feed intake, eating behaviour, rumination and nutrient digestibility. Three male sitatunga were fed a basal diet (chopped dehydrated lucerne, ground cereals, soyabean meal, vegetables and fruits) with access to meadow hay. The animals were allocated to 1 of 3 treatments according to 3 × 3 Latin square design and fed diets where 0, 50 or 100% of dry matter (DM) from basal diet was replaced with a pelleted cereal-based feed. DM intake of the basal diet and meadow hay, as well as overall DM intake and organic matter (OM) intake did not differ between treatments ( > 0.05). The eating rate (g DM · min–1) of the basal diet increased ( = 0.03) whereas eating time (min · day–1) and eating frequency (n · day–1) tended to decrease ( ≤ 0.07) linearly as pelleted feed inclusion in the diet increased. On the other hand, a tendency ( = 0.07) to longer time of hay intake (min · day–1) was observed with increasing inclusion of pelleted cereal-based feed in the basal diet. Rumination frequency (n · day–1), time (min · day–1) and rate (min · g–1 of DM intake) did not differ between treatments ( > 0.05). Apparent total tract digestibility of OM decreased linearly ( = 0.05) with increasing pellet inclusion in the diet. Pelleted cereal-based supplement used in the diet affects feeding behaviour and thus may affect health and welfare of sitatunga in zoological gardens.
The aim of this study was to determine the effect of pelleted cerealbased feed in the diet for addax antelope (Addax nasomaculatus) on feed intake and nutrient digestibility. Four addax females (3 years old) were allocated to 1 of 3 treatments according to 3 × 3 Latin square design and fed 3 diets. Each diet consisted of basal diet and meadow hay. In diet A basal diet consisted of a mixture of ground concentrates, chopped dehydrated lucerne, vegetables and fruits. In diet B 50% and in diet C 100% of dry matter (DM) provided with concentrates and chopped dehydrated lucerne in diet A was replaced with a pelleted cereal-based feed. Meadow hay was offered ad libitum. Two females (sisters) were kept together through the study and thus pen was used as an experimental unit. The diets were formulated to be similar for crude protein and crude fibre content. DM intake (DMI) of basal diet did not differ between treatments (P ≥ 0.52) whereas DMI of hay and total DMI linearly decreased with increasing pellet inclusion in the basal diet (P = 0.05 and P = 0.03, respectively). Digestibility of neutral detergent fibre tended to linearly decrease (P = 0.09) and digestibility of hemicellulose linearly decreased (P = 0.05) with increasing pellet inclusion in the diet. Pelleted cereal-based feed used in the diet for addax had a negative effect on roughage intake and digestibility of structured carbohydrates compared to non-pelleted feed of similar nutrient composition.
The aim of the study was to determine the effect of processing method of cereals used in the diet for nyala antelope (Tragelaphus angasii) on feed intake, eating behaviour, rumination and nutrient digestibility. The study was conducted according to Latin square design (4 × 4) on four adult nyala males. The animals were fed a basal diet consisting of a mixture of cereals (maize, wheat and oat), vegetables and fruits (1.25 kg/day), chopped dehydrated lucerne (0.5 kg/day) and meadow hay (ad libitum). Experimental treatments differed in processing method of cereals offered in the basal diet: 1. finely ground (GR); 2. steam-flaked (SF); or pelleted to obtain: 3. small (6mm diameter size; SP) or 4. large (12mm diameter size; LP) pellet. Feed intake did not differ between the treatments. Eating time of basal diet was longer for GR in comparison to SF and SP (P < 0.01), and also longer for LP in comparison to SP (P = 0.05), with amaximum difference between treatments of 11min/day. Eating rate (g dry matter/min) of basal diet was slower for GR in comparison to SF and SP (P ≤ 0.02). Consumption of meadow hay and chopped lucerne was not affected by cereal processing method. Digestibility of acid detergent fibre was greater (P = 0.05) and digestibility of dry matter and neutral detergent fibre tended (P ≤ 0.08) to be greater for GR than for SP. It can be concluded that, processing method of cereals may affect feeding behaviour of zoo ruminants.
Transforming growth factor β1 (TGF-β1) is a cytokine affecting cell proliferation and development, which also has an immunomodulatory activity. Correlations between polymorphisms of the TGF-β1 gene and clinical parameters of inflammatory bowel disease (IBD) were reported previously in adults. Here, we tested whether such correlations occur in pediatric patients suffering from IBD. One hundred and four pediatric IBD patients were involved in this study. Among them, 36 were diagnosed with Crohn's Disease (CD) and 68 were diagnosed with ulcerative colitis (UC). The control group consisted of 103 children, in which IBD was excluded. TGF-β1 levels were determined in plasma and intestinal mucosa samples. The presence of the TGF β1 protein and the amount of TGF β1 mRNA were estimated in intestinal mucosa by immunohistochemistry and reverse transcription Real-Time PCR, respectively. Four common polymorphisms of the TGF-β1 gene were investigated: -800G/A, -509C/T, 869T/C and 915G/C. No significant correlation between TGF-β1 genotypes and (i) TGF-β1 levels in plasma and tissue samples, (ii) TGF-β1 gene expression efficiency in intestinal mucosa, (iii) IBD clinical parameters and (iv) inflammatory activity could be detected in children suffering from IBD. We conclude that, contrary to previous suggestions, the four common polymorphisms of the TGF-β1 gene do not influence the susceptibility to or clinical parameters of IBD in the tested population of children.
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