Daring a study on Trypanosoma equiperdum propagation in adult male Wistar rats, four rats died spontaneously. Two of the dead animals were subjected to post mortem examination. From different organs of the rats, chlamydiae were isolated and confirmed by PCR and DNA sequencing as Chlamydophila psittaci. The results show that infection with Chlamydophila psittaci may occur in laboratory rats. Such outbreaks may have influence on the results of experimental studies. Chlamydial infections in laboratory animals also pose risk to humans (zoonosis).