Wyniki wyszukiwania

Sortuj według:

Ogranicz wyniki do:

Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik

Identification of point mutations in exon 2 of GDF9 gene in Kermani sheep

100%

Khodabakhshzadeh R., Mohammadabadi M. R., Esmailizadeh A. K., Moradi Shahrebabak H., Bordbar F., Ansari Namin S.

Polish Journal of Veterinary Sciences

2016

tom 19

nr 2

Screening the fertile ewes from national herds to detect the major genes for prolificacy is an effective way to create the fertile flocks. Growth differentiation factor (GDF) 9 is a member of the transforming growth factor β superfamily that is essential for folliculogenesis and female fertility. The aim of this study was to detect single nucleotide polymorphisms (SNPs) in exon 2 of GDF9 gene in Kermani sheep breed using PCR-SSCP. Genomic DNA was extracted from whole blood of collected samples using salting-out method. Whole exon 2 of GDF9 gene was amplified (634 bp and 647 bp fragments) using designed specific primers. The single stranded conformation polymorphism (SSCP) patterns of PCR products were studied using electrophoresis on acrylamide gel and silver-nitrate staining method. Finally, 4 banding patterns for the first primer pair and 4 banding patterns for the second primer pair were obtained. Also, indices of population genetic per SNP were calculated using Gen Alex 6.41 software. The sequencing results showed the presence of 3 mutations (SNP) (443, 477 and 721 positions) in the studied population.

Ograniczanie wyników

1 Polish Journal of Veterinary Sciences

1 Ansari Namin S.

1 Bordbar F.

1 Esmailizadeh A. K.

1 Khodabakhshzadeh R.

1 Mohammadabadi M. R.

1 Moradi Shahrebabak H.

1 2016

Wyniki wyszukiwania

Identification of point mutations in exon 2 of GDF9 gene in Kermani sheep