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Host and bacterial adhesion

100%

Slotwinska S. M.

Polish Journal of Veterinary Sciences

2013

tom 16

nr 1

Bacterial adhesion is an important step in tissue colonization and depends extensively on the surface properties of a bacterial cell. For many microorganisms the prerequisite for host body occupancy is a break in tissue continuity. The next step is ongoing tissue destruction by products of bacterial metabolism: microbial enzymes and toxins. This happens, for example, in the initial phase of periodontitis. The mechanisms of adhesion are related to the specific structures present on the bacterial cell surface. This article summarizes recent data about bacterial attachment to host cells.

Mikroflora kieszonek dziaslowych w zaawansowanym zapaleniu przyzebia

51%

Sawicka-Grzelak A., Meisel-Mikolajczyk F., Slotwinska S. M., Wierzbicka M.

Medycyna Doświadczalna i Mikrobiologia

1997

tom 49

nr 1-2

95-100

The aim of study was the evaluation of periodontal pockets microflora in patients with advanced periodontitis. From each subject 16-20 samples were taken using paper points. Pooled sample after 60 s. mixing was serially diluted in reduced BHI. For tolal cell counts and for the isolation of black pigmented anaerobes Brucella agar supplemented with 5% sheep blood, hemin, menadione, with and without Kanamycin-Vancomycin mixture and BM agar plates were used. For isolation of A. actinomycetemcomitans TSBV agar plates were used. Cultures were incubated in anaerobic chamber at 37°C for 7 days and TSBV agar plates in an atmosphere of 95% air-5% CO2 at 37°C for 5 days. Microorganisms were identified by Gram staining, colony morphology, fluorescence in UV-light, haemagglutination of 3% sheep erythrocytes, fermentation of sugars, production of indole, urease (API 20A), specific enzymes (Rapid ID 32A). Twenty seven subjects with clinically recognized periodontitis were examined. Microorganisms important in periodontitis were isolated from periodontal pockets of almost all examined subjects. The number of bacteria obtained from the sample of one patient ranged from 1•10 4CFU/ml to 3,6 •10 6 CFU/ml. Porphyromonas gingivalis was identified in the samples taken from 17 patients, Prevotella intermedia-19, Actinobacillus actinomycetemcomitans-11, Fusobacterium nucleatum-9, Peptostretococcus spp. -22.

Ograniczanie wyników

1 Medycyna Doświadczalna i Mikrobiologia

1 Polish Journal of Veterinary Sciences

2 Slotwinska S. M.

1 Meisel-Mikolajczyk F.

1 Sawicka-Grzelak A.

1 Wierzbicka M.

1 2013

1 1997

Wyniki wyszukiwania

Host and bacterial adhesion

Mikroflora kieszonek dziaslowych w zaawansowanym zapaleniu przyzebia