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Establishment of transgenic marigold using the floral dip method

100%

Cheng X., Huang C., Zhang X., Lyu Y.

Acta Physiologiae Plantarum

2019

tom 41

nr 08

The floral dip transformation method avoids tissue culture and regeneration processes of marigold, and the green fluorescent protein (GFP) is widely used in the detection of genetically modified organisms. In our study, the binary vector PCB260 containing the screening gene basta, the reporter gene GFP, and a 4 × 35S enhancer was transferred into marigold (Tagetes erecta) via Agrobacterium tumefaciens EHA105-mediated transformation using the floral dip method. After herbicide-resistance screening and genomic PCR testing, four transgenic lines were obtained in T₀ generation. In the T₁ generation, 15 transgenic plants showed fluorescence and were GFP-positive with phenotypic changes. The segregation ratio of mutant to normal plants was 1:3. Plant height, leaf length and width were significantly greater in the normal plants than in the mutant plants. Mutant plants did not bloom.

Lutein content in petals and leaves of marigold and analysis of lutein synthesis gene expression

88%

Cheng X., Zhao X., Huang C., Zhang X., Lyu Y.

Acta Physiologiae Plantarum

2019

tom 41

nr 07

Lutein is a bioactive natural carotenoid that is a member of the oxygen-containing carotenoids, and marigolds are the main raw material for the extraction of lutein. The lutein content in the leaves and petals of eight varieties of marigold including two inbred lines and six nuclear male sterile AB lines was measured using high-performance liquid chromatography (HPLC). The results showed that lutein was mainly stored in the petals of marigold, which had a lutein content 20 times higher than that in the leaves. Additionally, the lutein content in male parent 3 was the highest among the eight varieties of marigold in both petals and leaves. Correlation analysis showed that the expression level of the LCYE gene in the petals was positively correlated with the lutein content, with a correlation coefficient of 0.82, and the expression level of the LCYB gene in the leaves was positively correlated with the lutein content, with correlation a coefficient of 0.77. This study laid the foundation for the evaluation of parental resources and quality breeding of pigment marigold.

Ograniczanie wyników

2 Acta Physiologiae Plantarum

2 Cheng X.

2 Huang C.

2 Lyu Y.

2 Zhang X.

1 Zhao X.

2 2019

Wyniki wyszukiwania

Establishment of transgenic marigold using the floral dip method

Lutein content in petals and leaves of marigold and analysis of lutein synthesis gene expression