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1

A novel sheep gene, MMP7, differentially expressed in muscles from black-boned sheep and local common sheep

100%
Yonggang L., Shizheng G.
Journal of Applied Genetics
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2009
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tom 50
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nr 3
253-256
Differences in gene expression in muscles from Chinese black-boned sheep and local common sheep were investigated using mRNA differential display. One differentially expressed novel gene was identified through semi-quantitative RT-PCR, and the full-length cDNA sequence was then obtained using the rapid amplification of cDNA ends (RACE). The nucleotide sequence of this gene is not homologous to any of the known sheep genes, but it contains an open reading frame encoding a protein of 416 amino acids, which has high homology with matrix metallopeptidase 7 (matrilysin, uterine) (MMP7) of 10 species: bovine (93%), rhesus monkey (75%), human (74%), pig (73%), chimpanzee (73%), dog (73%), horse (72%), mouse (66%), rat (65%), and chicken (53%). Thus the novel gene can be defined as the sheep MMP7 gene. It was finally assigned to GeneID:100192317. The phylogenetic tree analysis revealed that the sheep MMP7 gene is closely related to the bovine MMP7. Our experiment is the first one to establish the primary foundation for further research on the sheep MMP7 gene.
2

A novel pig gene, CPNE1, differentially expressed in the muscle tissues from Wujin pigs and Large White Pigs

80%
Shifei P., Chuisi K., Yonggang L.
Animal Science Papers and Reports
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2012
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tom 30
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nr 2
The mRNA differential display technique was performed to investigate gene expression differences in the longissimus dorsi muscle from Wujin and Large White pigs. A fragment of one differentially expressed gene was isolated and sequenced. A complete cDNA sequence of the gene was obtained using the rapid amplification of cDNA ends (RACE) method. The open reading frame of this gene encodes a protein of 537 amino acids, which is homologous with the Copine I (CPNE1) of eight species: greater horseshoe bat (95%), mouse (92% ), rat (93%), cattle (95%), sheep (95%), rabbit (94%), human (95%) and dog (94%). This newly identified gene was respectively defined as the swine CPNE1 gene and had been assigned GeneID: 100233174. The phylogenetic analysis revealed that the swine CPNE1 gene has a closer genetic relationship with the CPNE1 gene of a dog. The tissue expression analysis indicated that the swine CPNE1 gene has a broad tissue distribution. The experiment described is the first to establish the primary foundation for further research on the swine CPNE1 gene.
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