A study has been conducted of reproduction of the rosy barb (Puntius conchonius) under controlled conditions. This fish are commonly kept in aquaria and are also a model fish used in research studies. It has been shown that rosy barb spawners should be kept in water at 20oC before reproduction, with 23oC being the optimum temperature for reproduction and eggs incubation. The interval between successive spawnings should range from 20 to 40 days. If it is too long, reaching 60 days, the quality of the gametes, expressed in the number of reproduced fish, is significantly reduced. The effectiveness of reproduction increases when there are more males than females in the spawning shoal, with the sex ratio being not lower than 2:1. Despite the small size of the fish, eggs can be also obtained by a semi-artificial method. The quality of the eggs thus obtained is not lower than that of the eggs produced in spontaneous spawning.