Wyniki wyszukiwania

1

In vitro culture of early globular-stage embryos of Brassica napus L.

100%

Goralski G., Przywara L.

Acta Biologica Cracoviensia. Series Botanica

|

1998

|

tom 40

Embryos of B. napus L. cl. Górczański from early globular to late globular stages (16-105 µm in length) were used for the culture. The embryo proper of the smallest ones consisted of 4-17 cells. A double-layer culture system was applied for culture of proembryos. The top layer contained 6% sucrose; the bottom was devoid of sugar. The osmotic pressure decreased gradually under culture due to the diffusion of osmotically active sucrose into the bottom layer. The medium used was based on ECM medium developed by Liu et al. (1993). The proembryos were placed on the surface of the bottom layer and then the top layer was placed over them, or else the proembryos were inoculated on the surface of the top layer. The percentage of embryo maturation was related to embryo length at the moment of inoculation. Among the embryos smaller than 50 µm, 3.7% matured, whereas 28.9% of the embryos larger than 50 µm produced seedlings. No embryo smaller than 30 µm grew in the culture. Coconut water (CW) had no significant influence on the effectiveness of culture, and an intact suspensor was not indispensable for the maturation of early globular embryos. Embedding proembryos in the top-layer medium was not necessary. The culture of proembryos inoculated on the surface yielded similar numbers of mature embryos.

2

Pelargonium zonale var. "Kleiner Liebling" as a model plant for haploid plant studies

81%

Tuleja M., Krzyzyk D., Goralski G., Plachno B. J.

Acta Biologica Cracoviensia. Series Botanica. Supplement

|

2016

|

tom 58

|

nr 1

3

Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik

Chromosome numbers and polyploidy in Polish angiosperms

81%

Gacek P., Goralski G., Joachimiak A. J.

Acta Biologica Cracoviensia. Series Botanica

|

2011

|

tom 53

|

nr 2

Our survey of data collected in the Chromosome Number Database for Polish angiosperms indicated that the 1,498 species with chromosome counts represent 40% of the total angiosperms (3,719) occurring in Poland, including 1,205 native species (53% of native species) and 194 anthropophytes (56% of anthropophytes). The chromosome numbers are known for all native species occurring in Poland within 298 genera and 46 families, and for all anthropophytes from 79 genera and 11 families. The remaining angiosperm groups are less explored: chromosome counts from Poland are known for 9% of cultivated species and 5% of ephemerophytes. According to generic basic chromosome numbers, 46.44% of Polish angiosperms have been classified as polyploid. By three different threshold methods, the contribution of polyploid plants to the Polish flora is 64.64%, 50.89% or 42.89%. Polyploidy is more common among indigenous than non-indigenous plants, and the ploidy distribution among plants from the Polish Tatras does not differ significantly from that observed in the rest of native Polish plants.

4

Cytological features of various microspore derivatives appearing during culture of isolated maize microspores

81%

Goralski G., Rozier F., Matthys-Rochon E., Przywara L.

Acta Biologica Cracoviensia. Series Botanica

|

2005

|

tom 47

|

nr 1

Microspore derivatives occurring in culture of maize microspores were studied by light and electron microscopy. The cultures showed a high degree of heterogeneity throughout the whole period of cultivation. Several types of androgenic structures at different developmental stages were observed, indicating a high level of asynchrony among microspores following the androgenic pathway. During the first 5 to 7 days of culture, multicellular structures developed inside the exine. At later stages of culture the developing microspores ruptured the pollen wall and formed structures variable in cellular characteristics. Three main types were distinguished: (i) one-domain structures consisting of small or large cells only; (ii) two-domain structures with large and small cells, the domains differing in size and cellular characteristics; (iii) callus-like structures. The two-domain structures seem to resemble zygotic proembryos which also contain two different parts, the suspensor and the apical region. The observed variability is most likely related to differences in gene activity during the inductive stage of androgenesis and the genetic properties of the microspores themselves, rather than to the conditions of in vitro culture.

5

Morphological and histological aspects of 2,4-D effects on rape explants [Brassica napus L. cv. Kana] cultured in vitro

81%

Slesak H., Popielarska M., Goralski G.

Acta Biologica Cracoviensia. Series Botanica

|

2005

|

tom 47

|

nr 1

The effect of different durations of exposure to 2,4-D on hypocotyls and cotyledons cultured in vitro was studied in Brassica napus L. cv. Kana. Organogenesis or callogenesis depended on the duration of explant exposure to MS medium supplemented with 2 mg/1 2,4-D (2,4-dichlorophenoxyacetic acid). Short treatment (1 and 3 days) with 2,4-D resulted in rhizogenesis on hypocotyls (100% and 98% of explants, respectively) and cotyledons (80% and 54% of explants, respectively). Adventitious shoots formed sporadically, with the highest frequency (14% of explants) on hypocotyls cultured 3 days on MS supplemented with 2,4-D and then transferred to hormone-free medium. Histological analysis clearly indicated that the basal part of hypocotyls is involved in root formation and callus production, and the apical part for shoots. Meristematic sites originated from groups of cells in the cortex layer (including cells of the endoderm), but the procambium, phloem and pericycle also showed meristematic activity. The present study indicated that the response of explants cultured on media containing 2,4-D at constant concentration depends on the duration of explant exposure to growth regulator.

6

Histological and SEM studies on organogenesis in endosperm-derived callus of kiwifruit [Actinidia deliciosa cv. Hayward]

81%

Popielarska M., Slesak H., Goralski G.

Acta Biologica Cracoviensia. Series Botanica

|

2006

|

tom 48

|

nr 2

Regeneration in endosperm-derived callus of Actinidla deliciosa cv. Hayward was documented by SEM combined with histology. Two kinds of callus, morphogenic and nonmorphogenic, were observed. Morphogenic callus consisted of compact cell clusters with epidermis-like tissue covered with a mucilaginous or continuous membranous layer, which partially disappeared, turned into fibrils, or became damaged. Regenerating shoots consisted of the apex and primordial leaves. Abnormal structures were also formed, frequently arrested in development. PAS reactions indicated that the mucilaginous layer and network present in intercellular spaces contains polysaccharides. Nonmorphogenic callus consisted of weakly attached cells without a covering membranous layer.

7

71%

Goralski G., Lafitte C., Bouazza L., Matthys-Rochon E., Przywara L.

Acta Biologica Cracoviensia. Series Botanica

|

2002

|

tom 44

Carbon source and osmotic pressure are known to be of great importance in cultures in vitro. We compared the effectiveness of sucrose, maltose and mixtures of glucose with fructose in androgenic culture of isolated maize microspores. Also we examined changes in media during culture, focusing on osmotic pressure and sugar composition. The results suggest that osmotic pressure and kind of sugar have a great influence on androgenesis induction but much less influence on the number of macroscopic structures formed. In media containing sucrose the osmotic pressure rises significantly due to sucrose hydrolysis. In other media tested, changes in osmotic pressure are much smaller or not significant. These results suggest that the factors involved in androgenesis induction are different from those responsible for macroscopic structure formation. Changes in sugar composition and osmotic pressure increases in sucrose-based medium might decrease the effectiveness of androgenesis in maize microspore culture.

13

Organogenesis in endosperm of Actinidia deliciosa cv. Hayward cultured in vitro

71%

Goralski G., Popielarska M., Slesak H., Siwinska D., Batycka M.

Acta Biologica Cracoviensia. Series Botanica

|

2005

|

tom 47

|

nr 2

An efficient procedure for shoot regeneration was obtained by endosperm culture in Actinidia deliciosa cv. Hayward. Mature endosperm cultured on MS medium supplemented with 2 mg/l 2,4-D and 5 mg/l kinetin developed callus with 80% efficiency. Callus was transferred on MS medium containing different plant growth regulators (2,4-D, TDZ, IAA, NAA, BAP, kinetin, 2iP) for regeneration. There were significant differences in regeneration response between medium supplemented with TDZ and medium with other hormones. Only medium containing TDZ stimulated shoot induction. The highest efficiency of shoot regeneration (avg. 6.2 shoots per culture) was on medium supplemented with 0.5 mg/l TDZ. The ploidy of callus and organs formed in endosperm culture was examined by flow cytometry. The results, peaks corresponding to 3C DNA amounts, confirmed the endospermal origin of callus, roots and shoots. Aneuploid and polyploid cells were found in endosperm-derived callus and regenerated organs.

14

Rumex tianschanicus x Rumex patientia - one of the most perspective energy plants: micropropagation protocol and flow cytometric analysis of nuclear DNA content

61%

Slezak H., Dela J., Goralski G., Ilnicki T., Sliwinska E., Popielarska-Konieczna M., Joachimiak A.

Acta Biologica Cracoviensia. Series Botanica. Supplement

|

2012

|

tom 54

|

nr 1

15

Extracellular matrix surface network is associated with non-morphogenic calli of Helianthus tuberosus cv. Albik produced from various explants

52%

Pilarska M., Popielarska-Konieczna M., Slesak H., Kozieradzka-Kiszkurno M., Goralski G., Konieczny R., Bohdanowicz J., Kuta E.

Acta Societatis Botanicorum Poloniae

|

2014

|

tom 83

|

nr 1

Helianthus tuberosus is economically important species. To improve characters of this energetic plant via genetic modification, production of callus tissue and plant regeneration are the first steps. A new, potentially energetic cultivar Albik was used in this study to test callus induction and regeneration. Callus was produced on leaves, petioles, apical meristems and stems from field-harvested plants but was totally non-morphogenic. Its induction started in the cortex and vascular bundles as confirmed by histological analysis. The surface of heterogeneous callus was partially covered with a membranous extracellular matrix surface network visible in scanning and transmission electron microscopies. The results clearly indicate that: (i) the morphogenic capacity of callus in topinambur is genotype dependent, (ii) cv. Albik of H. tuberosus proved recalcitrant in in vitro regeneration, and (iii) extracellular matrix surface network is not a morphogenic marker in this cultivar.

16

Phylogenetic relationships within Orobanche and Phelipanche (Orobanchaceae) from Central Europe, focused on problematic aggregates, taxonomy, and host ranges

52%

Piwowarczyk R., Denysenko-Bennett M., Goralski G., Kwolek D., Pedraja O. S., Mizia P., Cygan M., Joachimiak A. J.

Acta Biologica Cracoviensia. Series Botanica

|

2018

|

tom 60

|

nr 1

17

Study of embrogenesis in maize : different approaches and hypothesis on embroyogenic pathways

42%

Matthyson- Rachon E., Vergne P., Rogowski P., Lafitte C., Devaux P., Mol R., Goralski G., Piola F., Bonello J. F., Leduc N., Dumas C.

Acta Physiologiae Plantarum

|

1998

|

tom 20

|

nr 3 suppl.

Ograniczanie wyników

In vitro culture of early globular-stage embryos of Brassica napus L.

Pelargonium zonale var. "Kleiner Liebling" as a model plant for haploid plant studies

Chromosome numbers and polyploidy in Polish angiosperms

Cytological features of various microspore derivatives appearing during culture of isolated maize microspores

Morphological and histological aspects of 2,4-D effects on rape explants [Brassica napus L. cv. Kana] cultured in vitro

Histological and SEM studies on organogenesis in endosperm-derived callus of kiwifruit [Actinidia deliciosa cv. Hayward]

Androgenic development: a fascinating embryo formation process

Plant regeneration from liquid root culture of Rumex acetosa L. Analysis of genetic variability: preliminary studies

Organogenesis in in vitro culture of Actinidia deliciosa L. cv. Hayward endosperm

Chromosome numbers and polyploidy in life forms of Asteraceae, Poaceae and Rosaceae in Polish flora

Achievements in horizontal gene transfer studies in parasitic plants

Influence of sugars on isolated microspore development in maize [Zea mays L.]

Organogenesis in endosperm of Actinidia deliciosa cv. Hayward cultured in vitro

Rumex tianschanicus x Rumex patientia - one of the most perspective energy plants: micropropagation protocol and flow cytometric analysis of nuclear DNA content

Extracellular matrix surface network is associated with non-morphogenic calli of Helianthus tuberosus cv. Albik produced from various explants

Phylogenetic relationships within Orobanche and Phelipanche (Orobanchaceae) from Central Europe, focused on problematic aggregates, taxonomy, and host ranges

Study of embrogenesis in maize : different approaches and hypothesis on embroyogenic pathways