Wyniki wyszukiwania

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Mitodepressive and cytotoxic effects of short-term exposure to relatively small doses of pendimethalin evaluated by Allium test

100%

Tylicki A., Kowalska-Wochna E., Oscilowicz A., Ilasz R.

Polish Journal of Environmental Studies

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2010

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tom 19

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nr 1

193-199

Pendimethalin is an extensively used dinitroaniline herbicide. The half-life of pendimethalin in soil can be long, and accumulation of this herbicide in the environment is probable. Therefore, we investigated the influence of pendimethalin (0.033, 0.066, 0.099, 0.132 and 0.264 g/L) on cell division during short-term treatment (24 and 48 hours) using Allium test. We observed inhibition of root elongation after 48 hours of incubation with pendimethalin. This effect was caused by the inhibition of mitoses varying from 1/3 to 1/2 in the case of 0.033, 0.066 and 0.099 g/L of pendimethalin, and almost complete restriction of mitoses under higher concentrations. Pendimethalin caused mitotic disturbances (c-metaphases, anaphasal and telophasal chromosome bridges, multipolar anaphases) and interphase abnormalities (micronuclei, multinuclear cells). This effect was irreversible during a 48-hour postincubation in water. Mitotic disturbances were caused by abnormalities in the organization of the tubulin cytoskeleton. Results suggest that even small amounts of pendimethalin can be a danger for dividing cells and embryos.

2

Shoot meristem differentiation in shoot primordia culture (SPC) obtained from root primordia culture (RPC) of Solanum lycopersicoides Dun.

100%

Tylicki A., Burza W., Kuras M., Malepszy S.

Acta Physiologiae Plantarum

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2008

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tom 30

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nr 1

We present a unique example of conversion of the morphogenesis type (from rhisogenesis to shoot organogenesis) in in vitro cultures of Solanum lycopersicoides. Liquid shoot primordia cultures (SPC) were obtained from root primordia culture (RPC) on two kinds of MS-based media with BA. The first SMS₈, contained a full set of organic compounds; the second, ²SMS₈, was devoid of organic compounds except sucrose and edamine. Two weeks after passage of RPC onto both the media, disintegration of root primordia and cell aggregates began. After 8 weeks of cultivation on SMS₈ and ²SMS₈ media, SPC aggregates developed from meristematic cells located near the vascular tissue of disintegrating RPC aggregates. Initiation of shoot meristems started from meristematic cells centers which were localised under the surface of the newly formed SPC aggregates. The change in the type of morphogenesis occurred faster on medium SMS₈, but the SPC on medium ²SMS₈ was characterized by more frequent formation of shoots and plants (45% of aggregates, in the case of SPC on medium SMS₈, and 90% in the case of SPC on medium ²SMS₈). This fact was correlated with the structural organization of the meristematic centers. Our results indicate that an RPC system has high morphogenetic potential due to the continual presence of meristematic cells. The change in the type of morphogenesis was followed by a rebuilding of the aggregate structure on the basis of the meristematic cells already existing in RPC, which gave rise to SPC aggregates from which shoot meristems developed.

3

88%

Tylicki A., Burza W., Dziadczyk E., Kuras M., Malepszy S.

Zeszyty Naukowe Akademii Rolniczej w Krakowie. Sesja Naukowa

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1997

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tom 50

443-446

10

The effect of thiamine and oxythiamine on the rate of growth of Saccharomyces cerevisiae in vitro culture

88%

Tylicki A., Dobrzyn P., Czerniecki J., Lempicka A., Romaniuk-Demonchaux K.

Cellular and Molecular Biology Letters

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2001

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tom 06

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nr 3A

769

11

Characterisation of Solanum lycopersicoides Dun. root primoridia culture with plant recovery

88%

Burza W., Dziadczyk E., Tylicki A., Kuras M., Malepszy S.

Acta Physiologiae Plantarum

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2003

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tom 25

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nr 4

12

Puruvate and 2-oxoglutarate dehydrogenases as the essential regulatory points of metabolism

76%

Czerniecki J., Dobrzyn P., Tylicki A., Kondracikowska J., Czygier M., Strumilo S.

Acta Biochimica Polonica

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2006

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tom 53

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nr Supplement 1

Ograniczanie wyników

Mitodepressive and cytotoxic effects of short-term exposure to relatively small doses of pendimethalin evaluated by Allium test

Shoot meristem differentiation in shoot primordia culture (SPC) obtained from root primordia culture (RPC) of Solanum lycopersicoides Dun.

Kierowanie morfogeneza w kulturach zawiesinowych Solanum lycopersicoides Dun. III. Kultura primordiow pedowych

Changes in activity and kinetic properties of some key enzymes in rat tissues after the anabolic steroid treatment

Kompleks dehydrogenazy 2-oksoglutaranowej i wielopłaszczyznowa regulacja aktywności kompleksu

The mitotic activity of Allium cepa L. root cells and the disturbance of mitotic spindle formation induced by low doses of the herbicide stomp 330 EC

The role of the tubulin cytoskeleton of Solanum lycopersicoides Dun. protoplasts in the course of diploid plant regeneration

Regeneration of the cell wall by isolated protoplasts of Solanum lycopersicoides Dun is a selective process

Kierowanie morfogeneza roslin w kulturach zawiesinowych Solanum lycopersicoides Dun. IV. Powstawanie i struktura primordiow pedowych

The effect of thiamine and oxythiamine on the rate of growth of Saccharomyces cerevisiae in vitro culture

Characterisation of Solanum lycopersicoides Dun. root primoridia culture with plant recovery

Puruvate and 2-oxoglutarate dehydrogenases as the essential regulatory points of metabolism