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1
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
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Evaluation of the sensitivity of individual test organisms to residual concentrations of selected types of anticoccidial drugs

100%
Kozarova I., Mate D.
Bulletin of the Veterinary Institute in Puławy
|
2000
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tom 44
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nr 2
Anticoccidial drugs with antibiotic or antibacterial activity can be detected by microbiological agar diffusion methods based on the ability of certain drugs to inhibit the growth of sensitive strains of bacteria which act as test organisms and produce inhibition zones. In practice, microbiological agar diffusion methods form the basis of monitoring the residues of inhibitory substances and obtaining primary proof of the presence of residues in foods and raw materials of animal origin. The aim of the present study was to observe the sensitivity of individual test organisms to residual concentrations of selected types of anticoccidial drugs. In our study, four bacterial strains were tested by agar diffusion: Bacillus stearothermophilus var. calidolactis C 953, Bacillus subtillis BGA (at pH 6.0, pH 7.2 and pH 8.0), Micrococcus luteus ATCC 9341 and Bacillus cereus ATCC 11778. The following anticoccidial drug standards were used: amprolium, nicarbazin and furazolidone as chemical compounds, monensin and lasalocid as polyether ionophores, and sulfamethazine and sulfaquinoxaline as representatives of the sulfonamide group. The residual concentrations capable of inhibiting the growth of individual test organisms in our study were compared with maximum residual limits that have been established for these anticoccidial drugs. All anticoccidial drugs were tested using dilutions from 0.0001 mg/ml up to 1 mg/ml.
2

Comparison of the sensitivity of antibiotic residue screening methods to sulphonamide standards and their presumptive identification by para-aminobenzoic acid

81%
Janosova J., Kozarova I., Mate D., Okerman L.
Medycyna Weterynaryjna
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2008
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tom 64
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nr 05
The sensitivity of three microbiological antibiotic residue screening methods - the Four Plate Test (FPT), the Screening Test for Antibiotic Residues (STAR) and the Premi®Test - was compared for the detection of 10 different standards from the sulphonamide (SA) group. Phtalylsulphathiazole (PHT), sulphadimidine (SD), sulphaguanidine (SG), sulphachlorpyridazine (SCHP), sulphamerazine (SRZ), sulphamethoxazole (SMX), sulphanilamid (SAM), sulphanilic acid (SAC), sulphaquinoxaline (SQ) and sulphathiazole (STZ) were tested using the concentrations from 0.05 µg.ml⁻¹ to 1 µg.ml⁻¹. The detection sensitivity of the methods represented by minimum inhibiting concentrations (MIC) of SA standards was evaluated. The MIC of SAs represent the detection limits of methods (LOD) for individual substances. The MIC of SA standards detected by the FPT was 0.2 µg.ml⁻¹ for SMX, STZ, SQ and SRZ, 0.3 µg.ml⁻¹ for SCHP, and 0.4 µg.ml⁻¹ for SD. The MIC of SA standards detected by the STAR was 0.05 µg.ml⁻¹ for SMX, STZ, SQ and SCHP, 0.1 µg.ml⁻¹ for SRZ, and 0.3 µg.ml⁻¹ for SD. No detection sensitivity of either method was observed for SAM, SAC, SG and PHT standards. The MIC of SA standards detected by Premi®Test was 0.05 µg.ml⁻¹ for SMX, STZ, SQ, SCHP, SRZ and SD, 0.1 µg.ml⁻¹ for PHT, 0.2 µg.ml⁻¹ for SAM, and 0.3 µg.ml⁻¹ for SAC and SG. The results of the tests showed that Premi®Test is the most sensitive method to detect Sas, followed by the STAR and the FPT methods. The inhibitory effect of para-aminobenzoic acid (PABA) toward the residual concentrations of SA standards was evaluated. The reference PABA concentrations for the confirmation of the presence of all SAs detected by the FPT and the STAR was 1 µg.ml⁻¹ and 10 µg.ml⁻¹ for the Premi®Test. In the authors’ opinion, the presented concentration of PABA can be recommended for a reliable confirmation of the presence of SA residues even at concentrations that present a potential risk to human health.
3
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Bioprotective culture for safety and quality of cod salad in mayonnaise

71%
Zaborskiene G., Turek P., Nagy J., Laciakova A., Kozarova I.
Bulletin of the Veterinary Institute in Puławy
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2001
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tom 45
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nr 2
Bioprotective lactic acid from bacterial culture FloraCarn L-2u was tested for its biopreservative capacities to improve the quality and safety of cod salad, with additives: NaCl, acetic acid, sugar, mayonnaise, mustard and vegetables. The salad was divided into three portions: one untreated and two treated with a commercial Lactobacillus alimentarius biopreservative, inoculated in different concentrations (0.05 and 0.1g/kg). Lactic acid production, pH, development of the indigenous flora were examined for 14 days of aerobic storage in a refrigerator. The level of indigenous coliform bacteria, Staphylococcus aureus and moulds were reduced. The antibacterial effect of FloraCarn L-2u is attributed to lactic acid produced by this culture.
4

Utilisation of para-aminobenzoic acid [PABA] for the presumptive identification of sulphadimidine at its residue screening by using the microbiological four-plate test

71%
Kozarova I., Janosova J., Mate D., Pipova M., Jevinova P.
Bulletin of the Veterinary Institute in Puławy
|
2005
|
tom 49
|
nr 1
5

Study on properties of enterohaemorrhagic Escherichia coli 0157 isolates and their survival under different conditions

61%
Kovalikova M., Pipova M., Kantikova M., Kozarova I., Janosova J., Martonova M., Kovalik M.
Medycyna Weterynaryjna
|
2008
|
tom 64
|
nr 05
During the period of 2004-2006, 955 samples of food and clinical material were collected from the Slovak Republic and Belgium. Of the total number of 216 food samples originating from the Slovakia territory, the authors obtained 5 isolates (2.31%) of Escherichia coli O157. Three E. coli O157 (2.30%) isolates were obtained during the examination of 130 samples of slaughter animals from the Belgium territory. In Slovakia no occurrence of E. coli O157 was proved in any sample of clinical material or environment (altogether 36 samples). On the contrary, of 573 clinical and environmental samples from Belgium the presence of the respective pathogen was proved in 50 cases (8.73%). The authors studied some attributes of the recovered E. coli O157, confirmed by immunomagnetic separation (IMS) and polymerase chain reaction (PCR). The results indicate that the isolates are capable of surviving for 15 min at 70°C. The acidic environment, which is characteristic for fermentation of cheese (pH 4-4.5), had no devitalisation effect. The E. coli food isolates survived in a wide range of pH (2-11) while with clinical isolates the pH range of survival was from 2.5 to 10.5. The addition of NaCl in concentrations ordinarily used in food industry (3-6%) failed to inhibit the growth of pathogens.
6

Premi test - screening test for detection of sulphadimidine residues in eggs of laying hens

61%
Marcincak S., Hussein K., Mate D., Kozarova I., Sokol J., Zdolec N.
Medycyna Weterynaryjna
|
2006
|
tom 62
|
nr 02
The study investigated the presence of sulphadimidine residues in eggs after per oral administration of Sulfadimidin PG plv. sol. ad us. vet. (120 mg/hen/day) to laying hens. Premi®Test, a four-plate microbiological method, and HPLC were used to detect sulphadimidine residues. The positive findings of the Four-plate test (FPT) were confirmed by the results of Premi®Test. The absence of sulphadimidine residues was confirmed 72 hours after the last sulphadimidine administration by using the FPT. The presence of sulphadimidine residues was detected by Premi®Test within 8 days and by the FPT within 3 days after the last administration. The FPT test reported false-negative results for five days (kappa < 0.6) as compared with the results of Premi®Test. The study confirmed the conformity of results obtained by both Premi®Test and HPLC (kappa = 0.6).
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