Standard Pb, Cd, Zn and Cu additives were investigated for their effects on the fermenting activity of Lactobacillus helveticus and Lactobacillus casei. In milk enriched with a Lb. helveticus dairy culture and containing the standard Cu additive, activation of dairy bacteria could be observed. Standard amounts of Pb and Zn did not cause changes in titration acidity when compared to the control sample. On the other hand, increased growth inhibition was observed in these bacteria after adding 0.01 and 0.05 mg.kg⁻¹ Cd. Susceptibility of bacteria to increased Cd levels became evident in inhibition of the former. Pb-induced inhibition was obvious only after the addition of the 500% of the !egal level. Moderate activation was observed in samples containing Cu. The remaining elements did not cause changes in titration acidity.
The aim of our study was to observe the contents of nitrates and nitrites during the six stages of manufacture of Edam cheese. Samples were taken of raw milk, of pasteurized milk, of milk with nitrates added, of pressed cheese curd, of whey, of ripening cheese and the final product. The samples were drawn from a commercial operation in cheese factory in the eastern part of Slovakia. The mean natura NaNO₂ content in raw and pasteurized milk was 0.10 and 0.13 mg/kg, respectively. The mean natural NaNO₃ content in raw and pasteurized milk was 0.60 and 0.66 mg/kg, respectively. In milk with nitrate added the mean content was 96.58 mg/kg NaNO₃; the maximum value being 105.54 mg/kg NaNO₃. After pressing, the mean value of nitrate was found to be 15.45 mg/kg NaNO₃. A considerable quantity of nitrates passed into the whey, where the mean nitrate content was 76.58 mg/kg NaNO₃. The final product had a markedly decreased content of nitrates (1.69 mg/kg NaNO₃) and nitrites (0.61 mg/kg NaNO₂) when compared with the values in cheese during maturation (7.92 mg/kg NaNO₃ and 1.69 mg/kg NaNO₂).
The objective of the present study was to compare the differences and regularities in social behavior of newly mixed piglets under the conditions of large-scale commercial pig production. Three methods of grouping piglets at weaning (31-34d) into a 12-member weaned pool were studied: 6 pigs from 2 litters (6 × 2); 4 pigs from 3 litters (4 × 3); 3 pigs from 4 litters (3 × 4). Each method had three replicates and the weaned piglet body weight ranged from 7.4 up to 9.8 kg. Agonistic behavior was recorded over three observation periods: 0-1.5 h immediately after mixing; 1 h, 4-5 h later the same afternoon; 5 days later for 1 h. Both contact (head thrusting, biting and pushing) and non-contact (chasing, threatening and displacement) agonistic behavior were recorded. Contact behaviors made the most significant contribution to obtain a new dominance status in the social hierarchy and decreased significantly in frequency over time. Subsequently, the rate of non-contact behavior also declined. During the first observation period covering the crucial time for establishing dominance hierarchy, structure 4 × 3 had significantly less head thrust than 6 × 2 or 3 × 4. Generally, 4 × 3 showed a lower rate of contact behavior compared to the other two groups as well as less non-contact behavior than 3 × 4, but it was similar to 6 × 2. It appears that the 4 × 3 structure may cause less physical encounters than the other two groupings.
Effect of both vitamin D₃ and cadmium (Cd) on chymotrypsin activity (ACHT) of laying hen has been studied. Ten month-old laying hens were fed a compound feed of HYD-10 ad libitum. The birds were divided into 8 groups (each group consisting of 6 birds) as follows: experimental groups G1, G3, and G5 were supplemented with Cd (0.3; 0.6; and 6.0 mg.kg⁻¹ CdCl₂, respectively) groups G2, G4, and G6 were supplemented with vitamin D₃ (100 I.U./day) at the same Cd concentrations. C₁ was control group without any supplements and C₂ was control group supplemented only with vitamin D₃. The study lasted 6 months. In groups G1-G4, ACHT activities in the end of the study were rather high (2097.4; 2984; 1805.4, and 2575.9 U.g⁻¹,respectively). The ACHT activity in groups G2, G4 and G6 was by little higher than that in groups G1, G3, and G5 without vitamin D₃ supplementation. The principle of the action of vitamin D₃ via Ca ions and calmodulin is further discussed.
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