Wyniki wyszukiwania

Sortuj według:

Ogranicz wyniki do:

Forma nerwowa zakażenia herpeswirusem koni typ 1 jako nowo pojawiająca się choroba zakaźna koni

100%

Ploszay G., Rola J., Zmudzinski J. F.

Medycyna Weterynaryjna

2012

tom 68

nr 02

Equine herpesvirus 1 (EHV1) is one of the most important infectious agents in horses. This virus causes inflammation of the upper respiratory tract, pneumonia, abortion, death of newborn foals and encephalomyelitis known as Equine Herpesvirus Myeloencephalopathy (EHM). In recent years there has been a marked increase in the incidence of EHM caused by infection with neuropathogenic strains of EHV1. For this reason, some experts believe that EHM should be classified as a newly emerging infectious disease. Although this disease is less frequently observed than the other clinical forms of EHV1 infection, it may cause serious economic losses in breeding horses and have a very negative impact on the functioning of riding schools, racetracks and veterinary hospitals. This review discusses selected aspects of EHM, such as the link between the neurologic form of the disease and the EHV1 genotype, clinical signs, and methods of diagnosis and prevention.

Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik

First report on equine herpesvirus type 4 isolation in Poland – evaluation of diagnostic tools

100%

Ploszay G., Rola J., Larska M., Zmudzinski J. F.

Polish Journal of Veterinary Sciences

2013

tom 16

nr 3

Upper respiratory tract infections are still a serious problem in breeding and racing horses. The most common virological factors are EHV1 and EHV4, which are both a major cause of secondary infections. High EHV4 seroprevalence in Polish horses indicates a high transmission rate of this pathogen among horses and increases the need for proper diagnostics. The aim of this study was to develop a reliable laboratory diagnostic scheme for upper respiratory tract infections and to describe the first isolation of EHV4 in Poland. Twenty one nasal swabs collected from young horses under the age of 2 years showing clinical signs of equine rhinopneumonitis were tested with duplex PCR for simultaneous detection and differentiation between EHV1/EHV4. Positive samples were then subjected to virus isolation in Vero cells. Additionally, real-time PCR was developed which allowed viral copy numbers to be quantified and enabled defining that a DNA load below 10³ copies per 1 ml of the sample reflected latent infection or decline of the disease. However, the sensitivity of traditional PCR proved to be sufficient in the diagnostic of the lytic infections and allowed identification of 10 EHV4 infected horses from which 3 strains were successfully isolated in cell culture. Another four EHV4 positive results were obtained by real-time PCR; but, a high Ct (threshold cycle) and a low virus DNA copy number suggested a latent infection. This report describes the first successful isolation of EHV4 from Polish horses.

Ograniczanie wyników

1 Medycyna Weterynaryjna

1 Polish Journal of Veterinary Sciences

2 Ploszay G.

2 Rola J.

2 Zmudzinski J. F.

1 Larska M.

1 2013

1 2012

Wyniki wyszukiwania

Forma nerwowa zakażenia herpeswirusem koni typ 1 jako nowo pojawiająca się choroba zakaźna koni

First report on equine herpesvirus type 4 isolation in Poland – evaluation of diagnostic tools