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Appropriate irrigation and nitrogen fertilization, along with suitable crop management strategies, are essential prerequisites for optimum yields in agricultural systems. This research attempts to provide a scientific basis for sustainable agricultural production management for the North China Plain and other semi-arid regions. Based on a series of 72 treatments over 2003-2008, an optimized water and nitrogen scheme for winter wheat/summer maize cropping system was developed. Integrated systems incorporating 120 mm of water with 80 kg N ha-1 N fertilizer were used to simulate winter wheat yields in Hebei and 120 mm of water with 120 kg N ha-1 were used to simulate winter wheat yields in Shandong and Henan provinces in 2000-2007. Similarly, integrated treatments of 40 kg N ha-1 N fertilizer were used to simulate summer maize yields in Hebei, and 80 kg N ha-1 was used to simulate summer maize yields in Shandong and Henan provinces in 2000-2007. Under the optimized scheme, 341.74 107 mm ha-1 of water and 575.79 104 Mg of urea fertilizer could be saved per year under the wheat/maize rotation system. Despite slight drops in the yields of wheat and maize in some areas, water and fertilizer saving has tremendous long-term eco-environmental benefits.
In the present work we investigated the applications of chitosan as a bubble surface modifier in the coagulation dissolved air flotation (DAF) process for river water treatment. Zeta potentials of the residual colloidal particles in floated water and other typical water quality indexes measurements reinforced the fact that micro-bubble surface was successfully modified by dosing chitosan in a saturation system. The flotation efficiencies of both natural organic matters (NOM) and particles were obviously enhanced via chitosanmodified bubbles and the effects became more remarkable after the pH of saturation water was adjusted from 7.0 to 5.5, especially for NOM removal. Moreover, the influence of chitosan on DAF performance, as a coagulant aid, was also evaluated. The coagulation-aiding effects of chitosan were equal to acting as a bubble modifier at pH 7.0, but less efficient than the bubble modification DAF at pH 5.5. In addition, the effects of molecular weight (MW) and deacetylation degree (DD) of chitosan on its performance in both dosing strategies were discussed in detail. These findings highlight the advantages of bubble surface modification in surface water treatment and provide an alternative for DAF process optimization.
The acoustic structure of echolocation pulses emitted by Japanese pipistrellePipistrellus abramus (Temminck, 1840) bats during different phases of aerial hawking is described here for the first time. Behavioural observations of the foraging flight in conjunction with acoustical analysis of echolocation pulses indicated a flight path consisting of four distinct phases following the reconnaissance or search phase. Short (∼4.68 ms) and relatively broadband frequencymodulated (FM) pulses (∼23.55 kHz bandwidth) were emitted at a repetition rate of 15 Hz during presumed target approach. Presumed insect capture consisted of an early and a late buzz phase. Both buzz types were emitted at high repetition rates (111 Hz in early to 222 Hz in late) and consisted of very short, broadband FM pulses (1.26 ms in early to 0.3 ms in late). There was also a characteristically sharp drop in both the peak and terminal frequencies of each echolocation pulse during the transition from early to late buzz. No pulses were recorded during the final phase of foraging referred to as a “post-buzz pause”. Thus the foraging behaviour of this species consisted of five sequential phases involving four broad types of echolocation pulses.
This work compared the sensitivity of three cyanobacteria (Anabaena flos-aquae, Microcystis flosaquae and Mirocystis aeruginosa) as well as five green algae (Pseudokirchneriella subcapitata, Scenedesmus quadricauda, Scenedesmus obliquus, Chlorella vulgaris and Chlorella pyrenoidosa) to four pesticides through 96h short-term chronic tests. The results showed that the toxicity of the pesticides to the organisms increased in the order: propiconazol > isoprocarb > flumetralin > propargite. A wide variation in toxicity response of the organisms was observed. The sensitivity of the organisms varied by over one order of magnitude for propargite, by over two orders of magnitude for isoprocarb and propiconazol, and by over three orders of maginitude for flumetralin. Compared to green algae, cyanobacteria were less sensitive. This may result in the alteration of green algae dominated species to those dominated by cyanobacteria, stimulating to cyanobcterial bloom during a certain period.
We characterized 716 sites (including 22 beds, 60 foraging and 604 movement sites) used by red deerCervus elaphus xanthopygus Milne-Edwards, 1867 in the Wandashan Mountains, northeastern China in the winter period. We used covariates for vegetation, topography, disturbances by other ungulates, and disturbance by humans to develop movement, forage and bed site resource-selection models. We used an information-theoretic approach to select the top 5 models for movement, forage and bed site occurrences respectively. The three most parsimonious autologistic models were good predictors of movement, forage and bed occurrence of this species. Vegetation covariates were important components of all models. We recorded avoidance responses by the behaviours of species to each disturbance type (ie, villages, forest roads and abandoned roads); bed site occurrences were most sensitive to all human disturbances for their lowest odds encountered. Across all parsimonious models, villages have the largest negative effect on movement, foraging and bed occurrences. Movement and bedding behaviours were also affected by other ungulate disturbances. Altitude had slightly negative effect on movement and foraging behaviours. Ridges, topographic aspect and shrub stands were all correlated with bed-site selection. Although different behaviours may be associated with different microhabitat features, behaviours of red deer responded similarly to the same human disturbance on a broad scale. Based on the observations above, we believe that red deer are avoiding human-altered habitat for movement, bedding and foraging because of disturbances during the critical late winter period. Furthermore, various behavioural resource selection models and corresponding graphs of important habitat disturbances can be used to guide and evaluate future development proposals.
Eastern roe deerCapreolus pygargus bedfordi Thomas, 1908 occurred throughout China historically, but today are only found in 7 provinces. Their populations in China have been greatly impacted by human disturbances. Little is known about what kinds of human disturbances impact their distribution and resource requirements for survival. A survey was conducted over 20 661 ha located at the Erkehe Forestry Farm in the Lesser Khingan Mountains, northeastern China. Field work lasted from January to March, 2006 and January to March, 2007, and a total of 613 plots were visited along 28 sampling transects. Predictive models of easter roe deer resource selection were developed with or without the inclusion of human disturbance factors. Whether human disturbance exists or not, eastern roe deer retained strong links to patch size and patch density of low shrub and swamp, and the probability of moose occurrence. Low shrub and swamps created after logging provide more abundant annual shoots for food, and were vital for survival in harsh winters. When human disturbance was not modeled, eastern roe deer avoided higher density of bothBetula platyphylla andLarix gmelinii patches at a landscape scale, and larger mixed coniferous and broad-leaf patchs at local scales. Once human disturbances existed, eastern roe deer occurred in areas with higher NDVI and they had a considerably lower probability of presence in areas associated with roads and with a forest harvest interval of 4 years. Consequently, to predict effects of human disturbance on eastern roe deer spatial distribution, models need to incorporate effects of other competitive species, multiple spatial scale resource variables respectively, which will provide more value information for management and use of eastern roe deer population.
Eight founders and thirty-one descendants were sampled as the Founder group and the Offspring group respectively from a captive population of Amur tigerPanthera tigris altaica Temminck, 1844 for population genetic analysis with RAPD and ISSR markers. Integrated with demographic data during the initial recovery stage, results showed: (1) increasing the population size (N) and the effective population size (N e) greatly retard lose of genetic variation induced mainly by genetic drift and selection; (2) recombination and admixture could cause the Offspring group (5.711%) and the Founder group (10.383%) to hold different linkage disequilibrium (LD); (3) further Ohta’s variance analysis indicated genetic drift (87.3%) and epistatic selection (12.7%) maintained LD in population, whereas GENEDROP analysis supported epistatic selection largely derived from artificial selection of managers; (4) both Tajima’s test and Fu’s test confirmed the statistic neutrality of genetic markers used, moreover the positive value of Tajima’sD (0.090) together with the result that π (25.286) was bigger than ϑ (24.898) revealed the Founder group was admixture population, while the negative Tajima’sD value (−0.053) together with the result that π (23.679) was less than ϑ (23.912) disclosed the Offspring group experienced selective sweep.
This work examines the effects of six pesticides on five green algae: Pseudokirchneriella subcapitata, Scenedesmus quadricauda, Scenedesmus obliquus, Chlorella vulgaris and Chlorella pyrenoidosa. The re­sults indicate that the decreasing order of the average toxicity to five algae was: fluazinam > propineb > maneb > mancozeb > zineb > bromoxynil octanoate. However, according to sensitivity magnitude, the decreasing order of the ecological risk was: Maneb > bromoxynil octanoate > propineb/fluazinam > zineb/ mancozeb. There was a strong variance between toxicity and ecological risk.
This study evaluated the removal characteristics of tanic acid (TA) adsorbed on MIEX resin by batch experiments. MIEX resin can effectively remove TA from raw water at pH 6-9. Chloride and sulfate have adverse effect on the removal of TA. Conversely, bicarbonate can further the removal of TA. The Elovich model is the most suitable for depicting the kinetic process, and liquid film diffusion dominates the adsorption rate. The Freundlich model is reliable for describing the adsorption equilibrium. Adsorption is an endothermic, entropy-driving, and thermodynamically spontaneous process. The energy changes confirm the physical adsorption and dominate adsorption behavior. The sodium chloride solution (0.5%) can effectively regenerate the MIEX resin saturated TA, and the regenerated resin can be used circularly. Therefore, MIEX resin is a promising adsorbent for the removal of TA from raw water.
This study examines the hypothesis that soil respiration fluxes are always positive, neglecting negative fluxes in arid regions that characterize more than 30% of Earth’s total land area. To cut down uncertainty, we focus on non-vegetated areas at a typical, large arid region (Central Asia). Soil respiration fluxes were reconciled as a direct sum of influxes (CO₂ fluxes entering soils) and effluxes (CO₂ fluxes released from soils). It was indicated that the annual average of effluxes was only 8% higher than that of influxes in 1979-2011. At typically alkaline sites (soil pH>9.5), extreme local annual average of soil respiration fluxes are negative. Therefore, negative soil respiration fluxes in arid regions are unneglectable. Although the soil respiration flux is useful as a measure of CO₂ effluxes from the soils and CO₂ influxes to the soils, its value as a measure of ecosystem processes is very much limited.
ZNF300 was recently identified as a member of the human KRAB/C2H2 zinc finger protein family. Little is known about the role of ZNF300 in human gene regulation networks. In this study, the DNA-binding property of ZNF300 was further analyzed. We found that the recombinant ZNF300 could bind to the binding site 5′-GCGGGGGCG-3′ of Egr1, another member of the KRAB/C2H2 zinc finger protein family. Similarly, recombinant Egr1 also showed a similar binding affinity to the ZNF300 binding site 5′-CTGGGGGCG-3′. Bioinformatics analysis revealed that there is an overlapping ZNF300/Egr1 binding site in the human IL-2Rβ promoter region, which was previously known to be recognized by endogenous Egr1. Electrophoretic mobility shift assays showed that endogenous ZNF300 could also bind to this site. A transient transfection assay revealed that both ZNF300 and Egr1 could transactivate the IL-2Rβ promoter, and that the activation was abrogated by a mutation of residues in the overlapping ZNF300/Egr1 binding site. Co-expression of ZNF300 and Egr1 led to enhanced IL-2Rβ promoter activity. Thus, ZNF300 is likely to be another regulator of the human IL-2Rβ promoter.
The Chinese coking industry (CCI) is currently facing a great challenge on reducing greenhouse gas (GHG) emissions. Our study was set up to assess the GHG emission characteristics of 10 representative coke enterprises and analyse the factors of fuel type, production scale, loading method, oven model, loading rate, etc. The research results showed that fuel gas type, oven model, and loading rate had obvious impacts on the carbon emission intensity of coking plants, while the production scale and the loading method had little effects. The carbon emission intensity of the coking plant using mixed fuel gas was much higher than the plant using coke oven gas as fuel. The carbon emission intensity of the 6.0 m coke oven was 0.7 times higher than that of the 4.3 m coke oven. And the emission intensity of the clean heat recovery coke oven was the highest. An infrequent low loading rate would increase the direct carbon emission intensity signifi cantly. The research results will help the government to compare the emission intensity of the coking industry and make policies about carbon emission intensity reduction.
The fruits of Siraitia grosvenorii have been used as a natural sweetening agent for centuries and have also been reported to aid diabetic patients. Mogroside V is the primary sweet compound in S. grosvenorii fruit. In this study, we show that different pollen sources had a direct influence on the mogroside V content in S. grosvenorii fruit. The changes in the activities of carbohydrate metabolism-related enzymes during fruit development were examined, as well as the results of linear regression analysis of mogroside V and variables such as the carbohydrate content and activities of related enzymes. This indicated that sucrose metabolism was significantly correlated with mogroside V contents, and higher sucrose accumulation results in higher mogroside V levels. In addition, our results revealed an obvious effect of metaxenia on carbohydrate accumulation, sucrose and starch metabolism-related enzyme activities, which revealed the reason for the effect of metaxenia on the accumulation of mogroside V in S. grosvenorii fruit. These results suggest that the effects of metaxenia should be considered in future breeding programs.
Key message: HSF family transcription factors were analyzed in genome of Chinese cabbage. Chromosomal locations showed that duplication might result in expansion. Response to abiotic stresses was elucidated in Chinese cabbage varieties. Abstract: The major heat shock factors regulating the heat stress response are heat shock transcription factors (HSFs), which interact with heat shock elements. In this study, Chinese cabbage (Brassica rapa L. ssp. pekinensis) HSFs were comprehensively analyzed. A total of 52 HSF family genes were identified for phylogenetic relationships and motif analysis based on the genome sequence of Chinese cabbage. All HSFs were divided into classes A, B, and C. The chromosomal locations and gene duplications of these HSFs were also presented. Nine potential duplication events were found in Chinese cabbage chromosomes. Expression of three HSF genes in two varieties of Chinese cabbage using quantitative real-time PCR revealed that BraHSF039 and BraHSF043 were up-regulated under temperature and salt stresses treatments, and only BraHSF043 gene was also down-regulated under salt stress in ‘Lubaisanhao’. BraHSF001 gene was down-regulated in the ‘Lubaisanhao’ variety under heat and cold stresses, under drought stress in ‘Qingdao 87-114’. These results can serve as a foundation for further studies on HSFs in Brassica.
Suitable reference gene (RGs) is the prerequisite for accurate normalization of real-time quantitative PCR (RT-qPCR) data. However, previous results are diverse in various researches that focused on selecting stable RGs. This study aims at systematically assessing various RGs in plants under salt stress or drought stress by collection of geNorm rankings of genes, data transformation and statistic analysis. Although none of the analyzed genes can guarantee universally stable expressions in plant species under salt stress or drought stress, we found that 18S (18S ribosomal RNA) was generally the least stable gene under salt and drought stress. This gene should not be used as the RG in RT-qPCR. On the contrary, it is least risk to use EF1 for salt stress and TIP41 for drought treatment experiments. We compared the effects of salt and drought stresses on 7 frequently used RGs through paired-samples T test. The expression of Ubiquitin gene under drought stress is much more unstable than that under salt stress. The tested genes belonging to multigene family and having different stability could be one reason of variations in the published studies, which was supported by the analysis of expression profile of Salicornia europaea transcriptome. This is the first systematic assessment quantifying global stability of Rgs across plant species under salt stress and drought stress, which will improve our understanding of RGs and facilitate the future work on RGs selection.
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