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Production of essential oils from in vitro cultures of Caryopteris species and comparison of their concentrations with in vivo plants

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Luczkiewicz M., Jesionek A., Kokotkiewicz A., Migas P., Mardarowicz M., Szreniawa-Sztajnert A., Zabiegala B., Bucinski A.

Acta Physiologiae Plantarum

2015

tom 37

nr 03

The chemical composition of hydrodistilled essential oils obtained from aerial parts and roots of selected Caryopteris (‘bluebeard’) species (C. incana, C. mongolica, Caryopteris x clandonensis), as well as the newly established in vitro shoot and adventitious root cultures of the above plants, was analyzed by gas chromatography–mass spectrometry. Essential oil content and composition differed significantly depending on the type of plant material analyzed. Adventitious roots were characterized by the highest essential oil yield, reaching 1.8 % V/DW in Caryopteris x clandonensis. Limonene and cedrol were the main components of the essential oil derived from aerial parts of the intact plants (11.9–16.0 and 10.7–10.9 %, respectively), whereas the volatile fractions of the in vivo roots of all species contained large amounts of 3,5-bis(1,1-dimethyl)-phenol (12.9–26.2 %). 1,8-cineole, absent in the intact plant materials, was the dominating volatile constituent of the essential oils obtained from in vitro shoots (24.8–34.2 %). The volatile oil derived from adventitious root cultures consisted primarily of 1-octen-3-ol (19.7–31.5 %) and medicinally relevant diterpenoids: abietatriene and trans-totarol, which were accumulated in considerable quantities, especially in the adventitious roots of C. clandonensis (21.6 and 29.2 %, respectively).

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In vitro propagation of Rhododendron tomentosum - an endangered essential oil bearing plant from peatland

100%

Jesionek A., Kokotkiewicz A., Wlodarska P., Filipowicz N., Bogdan A., Ochocka R., Szreniawa-Sztajnert A., Zabiegala B., Bucinski A., Luczkiewicz M.

Acta Biologica Cracoviensia. Series Botanica

2016

tom 58

nr 2

Rhododendron tomentosum Harmaja (formerly Ledum palustre L.) is a medicinal peat bog plant native to northern Europe, Asia and North America. This plant has a distinctive aroma thanks to the presence of essential oil, to which it also owes its anti-inflammatory, analgesic, antimicrobial and insecticidal properties. However, in Europe R. tomentosum is classified as an endangered species, mainly due to degradation of peatlands. In the present work, the micropropagation protocol for R. tomentosum was established for the first time, providing both an ex situ conservation tool and a means of continuous production of in vivo and in vitro plant material for further studies. R. tomentosum microshoots were initiated from leaf explants and further multiplied using Schenk- Hildebrandt (SH) medium supplemented with 9.84 μM 2iP and 1.00 μM TDZ. The shoots were elongated on the SH medium supplemented with 24.6 μM 2iP and subsequently rooted using the perlite substrate saturated with half-strength Woody Plant medium supplemented with 1.0% sucrose and 4.92 μM IBA. The regenerated plants were hardened on the phytohormone-free SH medium and acclimatized using 3:1:1 deacidified peat:perlite:gravel substrate. The identity of the mother plant was confirmed at morphological and molecular levels and Random Amplified Polymorphic DNA (RAPD) method was implemented to assess the genetic fidelity of the regenerants. The essential oil content of the maternal plant, in vitro shoots and the regenerants was determined by steamdistillation, and the obtained volatile fractions were analyzed by GC/MS.

Ograniczanie wyników

1 Acta Biologica Cracoviensia. Series Botanica

1 Acta Physiologiae Plantarum

2 Bucinski A.

2 Jesionek A.

2 Kokotkiewicz A.

2 Luczkiewicz M.

2 Szreniawa-Sztajnert A.

2 Zabiegala B.

1 Bogdan A.

1 Filipowicz N.

1 Mardarowicz M.

1 Migas P.

1 Ochocka R.

1 Wlodarska P.

1 2016

1 2015

Wyniki wyszukiwania

Production of essential oils from in vitro cultures of Caryopteris species and comparison of their concentrations with in vivo plants

In vitro propagation of Rhododendron tomentosum - an endangered essential oil bearing plant from peatland