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Review of biotechnology research in alfalfa shows that molecular techniques are extensively being used for basic and applied research toward alfalfa improvement. Biotechnological approaches have been used in two major areas, genomics and transgenics. In genomics, molecular markers, structural and functional genomics allowed identification of genes of interest and their regulatory components. Alfalfa being obstinate to genetic and genomic analysis, comparative genomics is used for molecular and genetic dissection of various plant processes in alfalfa. Alternatively, transgenic approach involves incorporation of specific and useful genes into alfalfa to improve the traits of interest. Input traits to improve agronomic performance and output traits to improve forage quality, or to produce novel industrial/pharmaceutical proteins, are the focus of current transgenic research in alfalfa. However, transgenic approach is controversial requiring cautious experimental design to combat bioisafety concerns. Ideally, forage alfalfa needs to possess more fermentable carbohydrates, proteins with balanced amino acid profile that degrade slower in rumen, improved winter hardiness, better water use efficiency, pest resistance and no anti-quality factors. Concerted efforts are required to bring together maximum of these characteristic features into the alfalfa plant.
Increasing intensity and frequency of rainfall coupled with gradual retreating of glaciers due to climate change in Himalayan region likely to increase the risk of floods. A better understanding of risk zones which are vulnerable to flood disasters can be evolved from the detailed studies on slope, geomorphology and land use/ land cover pattern. Information of these parameters is an important input for the identification of vulnerable areas. Flood risk maps provide useful information about places that may be at risk from flooding. It offers a cost-effective solution for planning, management and mitigation strategies in risky areas. Traditional methods of flood risk mapping are based on ground surveys and aerial observations, but when the phenomenon is widespread, such methods are time consuming and expensive. The possible combination of DEM and other maps of area using an overlay operation method within the Geographical Information System (GIS) platform can lead to derivation and the understanding of spatial association between various parameters which could be used to predict flood risk zones. The study area i.e. Satluj River Basin has been broadly divided into five risk zones viz., very low, low, moderate, high and very high which helped to differentiate between areas that are at risk of different intensities of flood. The very high flood risk zone covers only 3.25 % of total study area, while the very low risk zone covers 13.63 %. The area falls within the very high and high risk constitutes 9.52 % of total basin area. Domain of moderate risk covers an area of 30.66 %. But the maximum area of river basin is constituted by low risk zone i.e. 46.19 %. Identification of such zones will help in timely adopting of mitigation and adaptation measures. Preparation of flood risk zoning maps also helps in regulating indiscriminate and unplanned land use practices in risky areas.
Prolong changes in climatic parameters may affect not only the geographical distribution of various vector borne diseases, but also is projected to have adverse effects on human health with regard to infectious diseases, particularly malaria. Malaria is perceived as the world’s most devastating infectious disease, and is responsible for very high morbidity and mortality. A number of socioeconomic, environmental and behavioral factors affect the disease prevalence. Despite these factors, the impact of climate on probability of malaria transmission remains contentious.
A six year field study was conducted from 2001-2002 to 2006-2007 at Punjab Agricultural University, Ludhiana, India to study the losses in seed yield of different Brassica species (B. juncea, B. napus, B. carinata, B. rapa and Eruca sativa) by the infestation of insect pests. The experiment was conducted in two different sets viz. protected/sprayed and unprotected, in a randomized block design, with three replications. Data on the infestation of insect pests, and seed yield were recorded at weekly intervals and at harvest, respectively. The loss in seed yield, due to mustard aphid and cabbage caterpillar, varied from 6.5 to 26.4 per cent. E. sativa suffered the least loss in seed yield and harboured the minimum population of mustard aphid (2.1 aphids/plant) and cabbage caterpillar (2.4 larvae/plant). On the other hand, B. carinata was highly susceptible to the cabbage caterpillar (26.2 larvae/plant) and suffered the maximum yield loss (26.4%).
Climate change arising from anthropogenic driven emissions of greenhouse gases has emerged as one of the most important environmental issues in the last two decades. One of the most significant potential consequences of climate change may be alteration in regional hydrological cycle and river flow regimes. Increased temperature is expected to increase the peak flows in snowfed rivers of Himalayas. The changing pattern of regional temperature on flood peaks deserves urgent and systematic attention over a basin which provides an insight view of historical trends. Lower reaches of Satluj River is selected for the present study. Testing the significance of observed trends in flood peaks has received a great attention recently, especially in connection with climate change. The data series available was 48 years (1967-2010). The records were subjected to trend analysis by using both non-parametric (Mann-Kendall test) and parametric (linear regression analysis) procedures. For better understanding of the observed trends, flood peaks were computed into standardised flood peak indices (SFPI). These standardised data series were plotted against time and the linear trends observed were represented graphically. The analysis of flood peaks at different observation stations in lower reaches of Satluj River showed a large variability in the trends and magnitudes. The trend analysis results of flood peaks and gauge heights indicate that the flood peaks at all sites i.e. Rampur, Suni and Kasol show increasing but statistically insignificant trends. The trends in gauge height at all sites are also showing increasing trend but Kasol is statistically significant at 95% confidence level. The fast melting of glaciers, incessant monsoon rainfall and the synchronisation of the discharge peaks are the main causes of river floods. The past flood peaks will help us to observe the frequency of occurrence of floods in certain region and to determine whether the flood peaks in the past have been same with that of the present or whether there is any deviation in the trend in relation to climate change. Such studies will help in designing mitigation and adaptation strategies towards extreme hydrological events.
Nothapodytes nimmoniana (J. Graham) (Icacinaceae), commonly known as Amruta is found in India particularly in Maharashtra, Goa, Kerala, Assam, Jammu and Kashmir as well as Tamilnadu areas. It is an important medicinal plant, the major source of a potent alkaloid, namely camptothecin, of a wide spectrum of pharmacological activities like anti-cancer, anti-HIV, antimalarial, antibacterial, anti-oxidant, anti-inflammatory, anti-fungal and also applied in the treatment of anaemia. Camptothecin is still not synthesized, therefore, its production entirely depends on natural sources. N. nimmoniana is one such plant which yields contain camptothecin in significantly high amount. The plant is gaining international recognition due to its diversified medicinal uses. It is subjected to excessive harvest. It has been categorized as a vulnerable and endangered plant. The present review encompasses the phytochemical, analytical, pharmacological, biotechnological, and other specific aspects of N. nimmoniana.
Cold environment favors long vegetative phase but also impose substantial loss by damaging reproductive functioning in chickpea. Field temperature below 10 °C is even more detrimental for reproductive development, enhances floral and pod abortion. In this study, contrasting chickpea varieties PDG3 and GPF2 were exposed to drought, recovered, and subsequently exposed to lethal cold stress ~ 4–5 °C with an aim to induce defense response against cold shock. Physiological, biochemical, and molecular signatures related to damage and defense, i.e., membrane damage, antioxidative enzymes, fatty acid desaturase (CaFAD2.1), and small HSPs (CaHSP18.5 and CaHSP22.7), were analyzed. Drought pretreatment/preconditioning maintained the membrane stability in the cold by managing malondialdehyde (MDA) content and lipoxygenase (LOX) activity. Improved mitochondrial functioning (TTC reduction), increased activity of catalase (CAT), ascorbate peroxidase (APX), and glutathione reductase (GR) proved better cellular functioning during cold exposure. The expression and activity of superoxide dismutase (CaSOD) were down-regulated in both varieties, but CaCAT, CaAPX, CaGR, and CaFAD2.1 expressions were up-regulated in GPF2. Small heat shock protein CaHSP22.7 was also up-regulated in drought preconditioned PDG3 and GPF2 and after cold shock. Drought pretreatment/preconditioning significantly improved membrane damage during cold exposure, induced antioxidative system, and up-regulated FAD2. This study also pointed the possible role of CaHSP22.7 in cold tolerance and CaHSP18.5 in drought stress. The sensitive variety (GPF2) was positively responsive to preconditioning as this variety showed improvement in defense-related parameters; however, genotypic variations were observed in PDG3.
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