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1
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
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Somatic embryogenesis of selected spruce species [Picea abies, P. omorika, P. pungens 'Glauca' and P. breweriana]

100%
Hazubska-Przybyl T., Bojarczuk K.
Acta Societatis Botanicorum Poloniae
|
2008
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tom 77
|
nr 3
189-199
Somatic embryogenesis was studied in four spruce species (Picea abies, P. omorika, P. pungens 'Glauca' and P. brewenana) to determine if this method can be used for in vitro propagation of coniferous trees. The highest frequency of initiation of embryogenic tissue was obtained when mature zygotic embryos were used as explants. It ranged then from 10.8% (P. brewenana) to 23.75% (P. omorika and P. pungens 'Glauca'). The frequency of embryogenic tissue initiation was strongly affected by medium composition, i.e. addition of appropriate auxins (2,4-D, NAA, Picloram) and sucrose concentration (10-20 g-1"1). A lower frequency was obtained in Picea omorika (10%) when megagametophytes (endosperms with immature zygotic embryos) were used as explants. No emryogenic tissue was produced from hypocotyls, cotyledons and needles. A satisfactory frequency was achieved with the use of somatic embryos of Picea abies (30%). The proliferation of embryogenic cell lines of spruces was affected by medium type. The experiments resulted in production of somatic plantlets of P. abies and P. omorika. This enables the application of this method of spruce micropropagation for genetic and breeding research or for nursery production.
2

Swiatowe tendencje wykorzystywania metod wegetatywnego rozmnazania drzew

100%
Szczygiel K., Hazubska-Przybyl T.
Leśne Prace Badawcze
|
2010
|
tom 71
|
nr 2
208-211
3

Indukcja, namnazanie i przechowywanie tkanki embriogennej swierka serbskiego w warunkach cieklego azotu

100%
Hazubska-Przybyl T., Bojarczuk K.
Biotechnologia
|
2010
|
nr 2
96-104
4
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
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Tree somatic embryogenesis in science and forestry

100%
Hazubska-Przybyl T., Bojarczuk K.
Dendrobiology
|
2016
|
tom 76
Somatic embryogenesis is the latest, and potentially the most efficient, method for the vegetative micropropagation of plants. Over the past three decades, numerous laboratory studies have investigated somatic embryogenesis of forest trees, yielding positive results for a number of economically important tree species. The first test trials were run and plantations were planted with interior spruce in the 90s by CellFor Inc. (Canada). However, at the beginning of the XXI century, the program to produce spruce and Douglas fir somatic seedlings was stopped for economic reasons. Thus, currently no operational program is ongoing except on a small scale in New Brunswick. In order to integrate somatic embryogenesis technology into operational reforestation programs, the production costs of forest tree somatic seedlings needs to be reduced, and the awareness of foresters and forest landowners that the material obtained through somatic embryogenesis is valuable needs to be increased. This awareness would enable implementation of this technology on a large scale for production and forest management throughout Europe including Poland. In this review, the importance of somatic embryogenesis in scientific research and in global and European forestry is presented. Our main aims are to provide basic information on the challenges in researching somatic embryogenesis of forest trees and to raise interest in this tree propagation technique in both scientists and foresters.
5
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Effect of PEG 4000 on maturation, germination and conversion frequency of Picea abies and P. omorika somatic embryos

81%
Wawrzyniak M., Hazubska-Przybyl T., Obarska A., Bojarczuk K.
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
|
2015
|
tom 96
|
nr 1
6

Induction and proliferation of embryogenic tissues of Serbian spruce (Picea omorika) and their maintenance in liquid nitrogen

81%
Hazubska-Przybyl T., Chmielarz P., Michalak M., Bojarczuk K.
Acta Biologica Cracoviensia. Series Botanica. Supplement
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2009
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tom 51
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nr 1
7
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In vitro cultures of Populus × canescens inoculated with various strains of ectomycorrhizal Paxillus involutus fungi under lead ion stress

81%
Szuba A., Karlinski L., Hazubska-Przybyl T., Mucha J.
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
|
2015
|
tom 96
|
nr 1
8
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
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Potential possibilities of the induction of European beech (Fagus sylvatica ) tissue cultures from various types of explants

81%
Hazubska-Przybyl T., Chmielarz P., Bojarczuk K.
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
|
2015
|
tom 96
|
nr 1
9
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
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Growth regulators and guaiacol peroxidase activity during the induction phase of somatic embryogenesis in Picea species

81%
Hazubska-Przybyl T., Ratajczak E., Kalemba E. M., Bojarczuk K.
Dendrobiology
|
2013
|
tom 69
Biochemical studies during the induction phase of somatic embryogenesis in Picea abies [L.] Karst. and P. omorika [Pančić] Purk. can supplement our basic knowledge of the developmental processes accompanying the formation of embryogenic tissues from explants. Such studies may also contribute to finding the markers specific to the early stages of somatic embryogenesis of spruce species and, consequently, to the optimization of the process of initiation of embryogenic tissues from different types of plant explants treated with various growth regulator combinations. In this paper the effect of certain growth regulator systems on enzymatic activity was studied. The analysis of guaiacol peroxidase activity (EC 1.11.1.7), based on the spectrophotometric method, showed that this activity was the lowest in mature zygotic embryos (explants) and significantly higher in 8-week-old embryogenic and non-embryogenic calluses treated with various combinations of growth regulators. In the newly initiated embryogenic tissue, the activity of this enzyme decreased and remained at a lower level during proliferation, irrespective of the applied growth regulator combination. The type and concentration of growth regulators used for the initiation and proliferation of embryogenic tissues had no statistically significant effect on peroxidase activity, although during the initiation often its increased level was observed in calluses treated with 2.4-D. Detection of guaiacol peroxidase activity in the induction phase of somatic embryogenesis proves its participation in this process. The subsequent change in its activity indicates that this peroxidase can be a biochemical marker of somatic embryogenesis of the tested spruce species.
10
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In vitro responses of various explants of Fagus sylvatica

81%
Hazubska-Przybyl T., Chmielarz P., Bojarczuk K.
Dendrobiology
|
2015
|
tom 73
There are limited published data on in vitro reproduction of Fagus sylvatica L. (European beech). This study was aimed to determine the efficiency of induction of somatic embryogenesis or organogenesis of beech from different types of explants in various culture conditions. Explants derived from immature, fresh seeds (collected in 2011 and 2013) and from mature seeds, stored at –10ºC and some stratified at 3ºC, were placed on induction media with various combinations of plant growth regulators: zeatin, 2,4-dichlorophenoxyacetic acid (2,4-D) and/or benzyladenine (BA). Initial cultures were kept in darkness or weak light (white fluorescent or blue-red LED). Limited success has been achieved in initiation of somatic embryogenesis. We obtained friable, yellow-white callus with characteristic PEM-like structures (cPEM-ls, from embryonic axes or fragments of immature embryos with embryonic axes), which may be an early developmental stage of embryogenic callus of Fagus sylvatica. This type of callus regenerated from explants incubated in darkness, mainly on WPM medium with addition of 6.8 μM zeatin or WPM and MSG media with 9.1 μM 2,4-D and 2.2 μM BA. The highest frequency of regeneration of callus with cPEM-ls was 5%. Instead, we succeeded to induce organogenesis from both immature and mature zygotic embryos and from embryonic axes. The best results were obtained for mature zygotic embryos incubated on ½WPM medium (half-strength Woody Plant Medium) with 9.1 μM 2,4-D and 2.2 μM BA. Adventitious buds were regenerated on up to 15% of the explants. The induced buds developed into shoots, enabling us to establish tissue cultures of beech. Induction of organogenesis from the tested explants was more efficient than induction of somatic embryogenesis.
11
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
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Structure of embryogenic tissues and accumulation of storage materials in somatic embryos of Picea abies and P. omorika

81%
Hazubska-Przybyl T., Bojarczuk K., Guzicka M.
Dendrobiology
|
2008
|
tom 60
19-28
Microscopic analysis of embryogenic tissues of Picea abies and P. omorika showed that the tissues differed in proportions of various types of proembryogenic masses, and in presence/absence of early somatic embryos, which suggests that they differed in embryogenic potential. On the basis of this study, embryogenic competence of tissues can be assessed at early stages of somatic embryogenesis, and thus the most efficient lines can be selected (one Picea abies and two P. omorika). In the course of development of somatic embryos embryogenic tissue was placed on a medium with abscisic acid (ABA), which affects an increase in osmotic pressure at the stage of maturation of somatic embryos and finally on the regeneration of cotyledonary embryos. In this stage of embryos development, storage materials were accumulated, which are used in specific metabolic processes during embryo development.
12

Effect of partial drying and desiccation on somatic seedling quality in Norway and Serbian spruce

81%
Hazubska-Przybyl T., Wawrzyniak M., Obarska A., Bojarczuk K.
Acta Physiologiae Plantarum
|
2015
|
tom 37
|
nr 01
Somatic embryo quality is still a problem for many researchers. To improve the efficiency of germination, special procedures are used, such as partial drying of somatic embryos at high relative humidity or desiccation in the presence of supersaturated solutions of salt. In this work, cotyledonary somatic embryos of Norway spruce (Picea abies) and Serbian spruce (P. omorika) were placed on culture media (ME or BM-5) to germinate. We found that after 4 weeks of incubation on these media, hypocotyl and radicle growth of control (non-dried) somatic embryos of both species was not adequate to yield seedlings able to acclimatize to greenhouse conditions. Therefore, somatic embryos were partly dried at relative humidity of 97 % or desiccated at relative humidity of 79 %, for 2 or 3 weeks, and then placed on the Margara (ME) medium. Partial drying of somatic embryos at the higher relative humidity (97 %) enabled an improvement of radicle growth of germinating somatic embryos in both species. The highest conversion rate (45 %) was obtained for embryos of Norway spruce maintained for 2 weeks at relative humidity of 97 %. This treatment contributed to the improvement of germination and conversion efficiency of somatic embryos of Norway spruce, regardless of the drying period. Improved radicle growth facilitated development of better quality seedlings of this spruce species. In Serbian spruce, we did not obtain seedlings of sufficient quality, due to poor hypocotyl growth. Desiccation at humidity of 79 % for 3 weeks proved to be lethal to somatic embryos of both species.
13

Effects of abscisic acid and an osmoticum on the maturation, starch accumulation and germination of Picea spp. somatic embryos

81%
Hazubska-Przybyl T., Kalemba E. M., Ratajczak E., Bojarczuk K.
Acta Physiologiae Plantarum
|
2016
|
tom 38
|
nr 02
14
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
Content available

Somatic embryogenesis of selected coniferous tree species of the genera Picea, Abies and Larix

80%
Szczygiel K., Hazubska-Przybyl T., Bojarczuk K.
Acta Societatis Botanicorum Poloniae
|
2007
|
tom 76
|
nr 1
Experiments on somatic embryogenesis in selected spruce, fir and larch species were performed to determine if this method of micropropagation enables production of quality seedlings for forest nurseries. High frequencies of embryogenic callus in spruce (23-31%) and fir (29%) were achieved when mature zygotic embryos were used as explants, while in larches (36%) only when megagametophytes (endosperms with immature embryos) were used. The possibility of somatic embryogenesis initiation also from somatic embryos (resulting in secondary and third generation of embryogenic callus) indicate high efficiency of this method of micropropagation. The best results at all stages of somatic embryogenesis (good proliferation of embryogenic callus, high rate of embryo regeneration and their survival) were obtained for Norway spruce, European larch and some hybrid larches.
15
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
Content available

Lasy z probówki. Czy to możliwe?

80%
Hazubska-Przybyl T.
Wszechświat
|
2020
|
tom 121
|
nr 10-12
16

Możliwość gromadzenia leśnych zasobów genowych w warunkach ex situ

51%
Chmielarz P., Suszka J., Bujarska-Borkowska B., Lewandowski A., Wawrzyniak M., Kotlarski S., Hazubska-Przybyl T., Litkowiec M., Plitta-Michalak B., Michalak M.
Biuletyn Instytutu Hodowli i Aklimatyzacji Roślin
|
2018
|
nr 283
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