The aim of our study was to evaluate whether in women with chlamydial cervicitis urogenital mycoplasmas and group B streptococci (GBS) colonization is found more often than among women with non-chlamydial cervicitis. This study included 351 (mean age 31.7 ±6.82) not pregnant, menstruating, sexually active women. We confirmed a high frequency (49.3%) of C. trachomatis infection among women with cervicitis. Cervical ectopia was confirmed in 26.5% of examined women, in half of them ectopia was associated with chlamydial infection. We did not notice differences in frequency of colonization by urogenital mycoplasmas and GBS among women with chlamydial and non-chlamydial cervicitis.